Acellular dermal matrix in skin wound healing in rabbits – histological and histomorphometric analyses
Autor(a) principal: | |
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Data de Publicação: | 2021 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/192055 |
Resumo: | OBJECTIVES: To analyze the histology and histomorphometry of healing associated with acellular dermal matrix in skin wounds in rabbits. METHODS: Twelve male rabbits were divided into two groups: the control group (CG) and the matrix group (MG). Three skin wounds with a total area of 20 20 mm were created on the dorsal region of each animal. Photographic records of the lesions taken over a 21-day period and use of the ImageJ program allowed calculation of the wound contraction rate. The lesions were biopsied on days 3, 14 and 21 for histomorphometric analysis to define the thicknesses of the dermis and epidermis (hematoxylin-eosin) and calculate the densities of type I and type III collagen (picrosirius). RESULTS: No significant difference in the healing rate was found between the groups (p40.05). The MG presented greater epidermal thickness on day 3 (po0.05) and on days 14 and 21 (po0.001). The MG presented greater dermal thickness throughout the study period (po0.05). The type I collagen density was higher in the MG throughout the study period (po0.05), and the type III collagen density was higher in the MG on days 3 and 14 (po0.05) and on day 21 (po0.001). CONCLUSION: The use of acellular dermal matrix increased the thickness of the dermal and epidermal layers and the amount of type I and III collagen during skin wound healing and did not alter the rate of wound contraction. |
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Clinics |
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Acellular dermal matrix in skin wound healing in rabbits – histological and histomorphometric analysesWound HealingRabbitDermal MatrixOBJECTIVES: To analyze the histology and histomorphometry of healing associated with acellular dermal matrix in skin wounds in rabbits. METHODS: Twelve male rabbits were divided into two groups: the control group (CG) and the matrix group (MG). Three skin wounds with a total area of 20 20 mm were created on the dorsal region of each animal. Photographic records of the lesions taken over a 21-day period and use of the ImageJ program allowed calculation of the wound contraction rate. The lesions were biopsied on days 3, 14 and 21 for histomorphometric analysis to define the thicknesses of the dermis and epidermis (hematoxylin-eosin) and calculate the densities of type I and type III collagen (picrosirius). RESULTS: No significant difference in the healing rate was found between the groups (p40.05). The MG presented greater epidermal thickness on day 3 (po0.05) and on days 14 and 21 (po0.001). The MG presented greater dermal thickness throughout the study period (po0.05). The type I collagen density was higher in the MG throughout the study period (po0.05), and the type III collagen density was higher in the MG on days 3 and 14 (po0.05) and on day 21 (po0.001). CONCLUSION: The use of acellular dermal matrix increased the thickness of the dermal and epidermal layers and the amount of type I and III collagen during skin wound healing and did not alter the rate of wound contraction.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2021-11-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/19205510.6061/clinics/2021/e2066Clinics; Vol. 76 (2021); e2066Clinics; v. 76 (2021); e2066Clinics; Vol. 76 (2021); e20661980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/192055/176981Copyright (c) 2021 Clinicsinfo:eu-repo/semantics/openAccessCarvalho-Júnior, José da Conceição Zanata, Fabiana Aloise, Antônio Carlos Ferreira, Lydia Masako 2023-07-06T13:04:03Zoai:revistas.usp.br:article/192055Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2023-07-06T13:04:03Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Acellular dermal matrix in skin wound healing in rabbits – histological and histomorphometric analyses |
title |
Acellular dermal matrix in skin wound healing in rabbits – histological and histomorphometric analyses |
spellingShingle |
Acellular dermal matrix in skin wound healing in rabbits – histological and histomorphometric analyses Carvalho-Júnior, José da Conceição Wound Healing Rabbit Dermal Matrix |
title_short |
Acellular dermal matrix in skin wound healing in rabbits – histological and histomorphometric analyses |
title_full |
Acellular dermal matrix in skin wound healing in rabbits – histological and histomorphometric analyses |
title_fullStr |
Acellular dermal matrix in skin wound healing in rabbits – histological and histomorphometric analyses |
title_full_unstemmed |
Acellular dermal matrix in skin wound healing in rabbits – histological and histomorphometric analyses |
title_sort |
Acellular dermal matrix in skin wound healing in rabbits – histological and histomorphometric analyses |
author |
Carvalho-Júnior, José da Conceição |
author_facet |
Carvalho-Júnior, José da Conceição Zanata, Fabiana Aloise, Antônio Carlos Ferreira, Lydia Masako |
author_role |
author |
author2 |
Zanata, Fabiana Aloise, Antônio Carlos Ferreira, Lydia Masako |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Carvalho-Júnior, José da Conceição Zanata, Fabiana Aloise, Antônio Carlos Ferreira, Lydia Masako |
dc.subject.por.fl_str_mv |
Wound Healing Rabbit Dermal Matrix |
topic |
Wound Healing Rabbit Dermal Matrix |
description |
OBJECTIVES: To analyze the histology and histomorphometry of healing associated with acellular dermal matrix in skin wounds in rabbits. METHODS: Twelve male rabbits were divided into two groups: the control group (CG) and the matrix group (MG). Three skin wounds with a total area of 20 20 mm were created on the dorsal region of each animal. Photographic records of the lesions taken over a 21-day period and use of the ImageJ program allowed calculation of the wound contraction rate. The lesions were biopsied on days 3, 14 and 21 for histomorphometric analysis to define the thicknesses of the dermis and epidermis (hematoxylin-eosin) and calculate the densities of type I and type III collagen (picrosirius). RESULTS: No significant difference in the healing rate was found between the groups (p40.05). The MG presented greater epidermal thickness on day 3 (po0.05) and on days 14 and 21 (po0.001). The MG presented greater dermal thickness throughout the study period (po0.05). The type I collagen density was higher in the MG throughout the study period (po0.05), and the type III collagen density was higher in the MG on days 3 and 14 (po0.05) and on day 21 (po0.001). CONCLUSION: The use of acellular dermal matrix increased the thickness of the dermal and epidermal layers and the amount of type I and III collagen during skin wound healing and did not alter the rate of wound contraction. |
publishDate |
2021 |
dc.date.none.fl_str_mv |
2021-11-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/192055 10.6061/clinics/2021/e2066 |
url |
https://www.revistas.usp.br/clinics/article/view/192055 |
identifier_str_mv |
10.6061/clinics/2021/e2066 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/192055/176981 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2021 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2021 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 76 (2021); e2066 Clinics; v. 76 (2021); e2066 Clinics; Vol. 76 (2021); e2066 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222765699039232 |