The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/100996 |
Resumo: | OBJECTIVE: Circulating microRNAs have been recognized as promising biomarkers for various diseases. The present study aimed to explore the potential roles of circulating miR-149, miR-424 and miR-765 as non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged (40–60-year-old) patients. METHODS: Sixty-five stable coronary artery disease patients (49–57 years old), 30 unstable coronary artery disease patients (49–58 years old), and 32 non-coronary artery disease patients (49–-57 years old) who were matched for age, sex, smoking habits, hypertension and diabetes were enrolled in this study. Total RNA was isolated from plasma with TRIzol reagent. Circulating miRNA levels were measured by quantitative real-time polymerase chain reaction. RESULTS: Circulating miR-149 levels were decreased 4.49-fold in stable coronary artery disease patients (1.18 ± 0.84) and 5.09-fold in unstable coronary artery disease patients (1.04 ± 0.65) compared with non-coronary artery disease patients (5.30 ± 2.57) (p |
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oai:revistas.usp.br:article/100996 |
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USP-19 |
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Clinics |
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The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients OBJECTIVE: Circulating microRNAs have been recognized as promising biomarkers for various diseases. The present study aimed to explore the potential roles of circulating miR-149, miR-424 and miR-765 as non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged (40–60-year-old) patients. METHODS: Sixty-five stable coronary artery disease patients (49–57 years old), 30 unstable coronary artery disease patients (49–58 years old), and 32 non-coronary artery disease patients (49–-57 years old) who were matched for age, sex, smoking habits, hypertension and diabetes were enrolled in this study. Total RNA was isolated from plasma with TRIzol reagent. Circulating miRNA levels were measured by quantitative real-time polymerase chain reaction. RESULTS: Circulating miR-149 levels were decreased 4.49-fold in stable coronary artery disease patients (1.18 ± 0.84) and 5.09-fold in unstable coronary artery disease patients (1.04 ± 0.65) compared with non-coronary artery disease patients (5.30 ± 2.57) (pHospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2015-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/10099610.6061/clinics/2015(04)07Clinics; Vol. 70 No. 4 (2015); 257-263Clinics; v. 70 n. 4 (2015); 257-263Clinics; Vol. 70 Núm. 4 (2015); 257-2631980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/100996/99660Copyright (c) 2015 Clinicsinfo:eu-repo/semantics/openAccessSayed, Ali Sheikh Md Xia, Ke Li, Fei Deng, Xu Salma, Umme Li, Tingbo Deng, Hai Yang, Dafeng Haoyang, Zhou Yang, TianLun Peng, Jun 2015-07-28T13:19:57Zoai:revistas.usp.br:article/100996Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2015-07-28T13:19:57Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients |
title |
The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients |
spellingShingle |
The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients Sayed, Ali Sheikh Md |
title_short |
The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients |
title_full |
The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients |
title_fullStr |
The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients |
title_full_unstemmed |
The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients |
title_sort |
The diagnostic value of circulating microRNAs for middle-aged (40–60-year-old) coronary artery disease patients |
author |
Sayed, Ali Sheikh Md |
author_facet |
Sayed, Ali Sheikh Md Xia, Ke Li, Fei Deng, Xu Salma, Umme Li, Tingbo Deng, Hai Yang, Dafeng Haoyang, Zhou Yang, TianLun Peng, Jun |
author_role |
author |
author2 |
Xia, Ke Li, Fei Deng, Xu Salma, Umme Li, Tingbo Deng, Hai Yang, Dafeng Haoyang, Zhou Yang, TianLun Peng, Jun |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sayed, Ali Sheikh Md Xia, Ke Li, Fei Deng, Xu Salma, Umme Li, Tingbo Deng, Hai Yang, Dafeng Haoyang, Zhou Yang, TianLun Peng, Jun |
description |
OBJECTIVE: Circulating microRNAs have been recognized as promising biomarkers for various diseases. The present study aimed to explore the potential roles of circulating miR-149, miR-424 and miR-765 as non-invasive biomarkers for the diagnosis of coronary artery disease in middle-aged (40–60-year-old) patients. METHODS: Sixty-five stable coronary artery disease patients (49–57 years old), 30 unstable coronary artery disease patients (49–58 years old), and 32 non-coronary artery disease patients (49–-57 years old) who were matched for age, sex, smoking habits, hypertension and diabetes were enrolled in this study. Total RNA was isolated from plasma with TRIzol reagent. Circulating miRNA levels were measured by quantitative real-time polymerase chain reaction. RESULTS: Circulating miR-149 levels were decreased 4.49-fold in stable coronary artery disease patients (1.18 ± 0.84) and 5.09-fold in unstable coronary artery disease patients (1.04 ± 0.65) compared with non-coronary artery disease patients (5.30 ± 2.57) (p |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-04-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/100996 10.6061/clinics/2015(04)07 |
url |
https://www.revistas.usp.br/clinics/article/view/100996 |
identifier_str_mv |
10.6061/clinics/2015(04)07 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/100996/99660 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 70 No. 4 (2015); 257-263 Clinics; v. 70 n. 4 (2015); 257-263 Clinics; Vol. 70 Núm. 4 (2015); 257-263 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222761743810560 |