Is there a relationship between endothelial nitric oxide synthase gene polymorphisms and ankylosing spondylitis?

Detalhes bibliográficos
Autor(a) principal: Sari, Ismail
Data de Publicação: 2013
Outros Autores: Igci, Yusuf Ziya, Can, Gercek, Taylan, Ali, Solmaz, Dilek, Gogebakan, Bulent, Akar, Servet, Eslik, Zeynep, Bozkaya, Giray, Akkoc, Nurullah
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/72126
Resumo: OBJECTIVE: Nitric oxide is produced by endothelial nitric oxide synthase, and its production can be influenced by polymorphisms of the endothelial nitric oxide synthase gene. Because candidate genes responsible for susceptibility to ankylosing spondylitis are mostly unknown and available data suggest that there may be problems related to the nitric oxide pathway, such as endothelial dysfunction and increased asymmetric dimethylarginine, this study aimed to assess the association of common endothelial nitric oxide synthase gene polymorphisms with ankylosing spondylitis. METHODS: One hundred ninety-four unrelated Turkish ankylosing spondylitis patients and 113 healthy without apparent cardiovascular disease, hypertension or diabetes mellitus were included. All individuals were genotyped by PCR-RFLP for two single-nucleotide polymorphisms, namely 786T>;C (rs2070744, promoter region) and 786 Glu298Asp (rs1799983, exon 7). Variable numbers of tandem repeat polymorphisms in intron 4 were also studied and investigated by direct electrophoresis on agarose gel following polymerase chain reaction analysis. The Bath ankylosing spondylitis metrology index of the patients was calculated, and human leukocyte antigen B27 was studied. RESULTS: All studied polymorphisms satisfied Hardy-Weinberg equilibrium. Sex distributions were similar between the patient and control groups. No significant differences were found in the distributions of allele and genotype frequencies of the studied endothelial nitric oxide synthase polymorphisms between patients and controls. There were no correlations between endothelial nitric oxide synthase polymorphisms, disease duration, Bath ankylosing spondylitis metrology index or human leukocyte antigen B27. CONCLUSION: The results presented in this study do not support a major role of common endothelial nitric oxide synthase polymorphisms in Turkish ankylosing spondylitis patients.
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spelling Is there a relationship between endothelial nitric oxide synthase gene polymorphisms and ankylosing spondylitis?Ankylosing SpondylitisEndothelial Nitric Oxide SynthaseNitric OxideInflammationAtherosclerosisOBJECTIVE: Nitric oxide is produced by endothelial nitric oxide synthase, and its production can be influenced by polymorphisms of the endothelial nitric oxide synthase gene. Because candidate genes responsible for susceptibility to ankylosing spondylitis are mostly unknown and available data suggest that there may be problems related to the nitric oxide pathway, such as endothelial dysfunction and increased asymmetric dimethylarginine, this study aimed to assess the association of common endothelial nitric oxide synthase gene polymorphisms with ankylosing spondylitis. METHODS: One hundred ninety-four unrelated Turkish ankylosing spondylitis patients and 113 healthy without apparent cardiovascular disease, hypertension or diabetes mellitus were included. All individuals were genotyped by PCR-RFLP for two single-nucleotide polymorphisms, namely 786T>;C (rs2070744, promoter region) and 786 Glu298Asp (rs1799983, exon 7). Variable numbers of tandem repeat polymorphisms in intron 4 were also studied and investigated by direct electrophoresis on agarose gel following polymerase chain reaction analysis. The Bath ankylosing spondylitis metrology index of the patients was calculated, and human leukocyte antigen B27 was studied. RESULTS: All studied polymorphisms satisfied Hardy-Weinberg equilibrium. Sex distributions were similar between the patient and control groups. No significant differences were found in the distributions of allele and genotype frequencies of the studied endothelial nitric oxide synthase polymorphisms between patients and controls. There were no correlations between endothelial nitric oxide synthase polymorphisms, disease duration, Bath ankylosing spondylitis metrology index or human leukocyte antigen B27. CONCLUSION: The results presented in this study do not support a major role of common endothelial nitric oxide synthase polymorphisms in Turkish ankylosing spondylitis patients.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2013-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/7212610.1590/clin.v68i3.72126Clinics; Vol. 68 No. 3 (2013); 305-309Clinics; v. 68 n. 3 (2013); 305-309Clinics; Vol. 68 Núm. 3 (2013); 305-3091980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/72126/75361Sari, IsmailIgci, Yusuf ZiyaCan, GercekTaylan, AliSolmaz, DilekGogebakan, BulentAkar, ServetEslik, ZeynepBozkaya, GirayAkkoc, Nurullahinfo:eu-repo/semantics/openAccess2014-01-28T17:05:36Zoai:revistas.usp.br:article/72126Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2014-01-28T17:05:36Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Is there a relationship between endothelial nitric oxide synthase gene polymorphisms and ankylosing spondylitis?
title Is there a relationship between endothelial nitric oxide synthase gene polymorphisms and ankylosing spondylitis?
spellingShingle Is there a relationship between endothelial nitric oxide synthase gene polymorphisms and ankylosing spondylitis?
Sari, Ismail
Ankylosing Spondylitis
Endothelial Nitric Oxide Synthase
Nitric Oxide
Inflammation
Atherosclerosis
title_short Is there a relationship between endothelial nitric oxide synthase gene polymorphisms and ankylosing spondylitis?
title_full Is there a relationship between endothelial nitric oxide synthase gene polymorphisms and ankylosing spondylitis?
title_fullStr Is there a relationship between endothelial nitric oxide synthase gene polymorphisms and ankylosing spondylitis?
title_full_unstemmed Is there a relationship between endothelial nitric oxide synthase gene polymorphisms and ankylosing spondylitis?
title_sort Is there a relationship between endothelial nitric oxide synthase gene polymorphisms and ankylosing spondylitis?
author Sari, Ismail
author_facet Sari, Ismail
Igci, Yusuf Ziya
Can, Gercek
Taylan, Ali
Solmaz, Dilek
Gogebakan, Bulent
Akar, Servet
Eslik, Zeynep
Bozkaya, Giray
Akkoc, Nurullah
author_role author
author2 Igci, Yusuf Ziya
Can, Gercek
Taylan, Ali
Solmaz, Dilek
Gogebakan, Bulent
Akar, Servet
Eslik, Zeynep
Bozkaya, Giray
Akkoc, Nurullah
author2_role author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sari, Ismail
Igci, Yusuf Ziya
Can, Gercek
Taylan, Ali
Solmaz, Dilek
Gogebakan, Bulent
Akar, Servet
Eslik, Zeynep
Bozkaya, Giray
Akkoc, Nurullah
dc.subject.por.fl_str_mv Ankylosing Spondylitis
Endothelial Nitric Oxide Synthase
Nitric Oxide
Inflammation
Atherosclerosis
topic Ankylosing Spondylitis
Endothelial Nitric Oxide Synthase
Nitric Oxide
Inflammation
Atherosclerosis
description OBJECTIVE: Nitric oxide is produced by endothelial nitric oxide synthase, and its production can be influenced by polymorphisms of the endothelial nitric oxide synthase gene. Because candidate genes responsible for susceptibility to ankylosing spondylitis are mostly unknown and available data suggest that there may be problems related to the nitric oxide pathway, such as endothelial dysfunction and increased asymmetric dimethylarginine, this study aimed to assess the association of common endothelial nitric oxide synthase gene polymorphisms with ankylosing spondylitis. METHODS: One hundred ninety-four unrelated Turkish ankylosing spondylitis patients and 113 healthy without apparent cardiovascular disease, hypertension or diabetes mellitus were included. All individuals were genotyped by PCR-RFLP for two single-nucleotide polymorphisms, namely 786T>;C (rs2070744, promoter region) and 786 Glu298Asp (rs1799983, exon 7). Variable numbers of tandem repeat polymorphisms in intron 4 were also studied and investigated by direct electrophoresis on agarose gel following polymerase chain reaction analysis. The Bath ankylosing spondylitis metrology index of the patients was calculated, and human leukocyte antigen B27 was studied. RESULTS: All studied polymorphisms satisfied Hardy-Weinberg equilibrium. Sex distributions were similar between the patient and control groups. No significant differences were found in the distributions of allele and genotype frequencies of the studied endothelial nitric oxide synthase polymorphisms between patients and controls. There were no correlations between endothelial nitric oxide synthase polymorphisms, disease duration, Bath ankylosing spondylitis metrology index or human leukocyte antigen B27. CONCLUSION: The results presented in this study do not support a major role of common endothelial nitric oxide synthase polymorphisms in Turkish ankylosing spondylitis patients.
publishDate 2013
dc.date.none.fl_str_mv 2013-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/72126
10.1590/clin.v68i3.72126
url https://www.revistas.usp.br/clinics/article/view/72126
identifier_str_mv 10.1590/clin.v68i3.72126
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/72126/75361
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 68 No. 3 (2013); 305-309
Clinics; v. 68 n. 3 (2013); 305-309
Clinics; Vol. 68 Núm. 3 (2013); 305-309
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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