Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/96957 |
Resumo: | OBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 µg/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip¯ Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip¯ Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. RESULTS: We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of GII compared to those of the control group. CONCLUSION: Our data suggest that melatonin supplementation decreases gene expression of cyclic adenosine monophosphate, which changes ovarian steroidogenesis. |
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oai:revistas.usp.br:article/96957 |
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Clinics |
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Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation OBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 µg/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip¯ Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip¯ Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. RESULTS: We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of GII compared to those of the control group. CONCLUSION: Our data suggest that melatonin supplementation decreases gene expression of cyclic adenosine monophosphate, which changes ovarian steroidogenesis. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2015-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/9695710.6061/clinics/2015(02)12Clinics; Vol. 70 No. 2 (2015); 144-151Clinics; v. 70 n. 2 (2015); 144-151Clinics; Vol. 70 Núm. 2 (2015); 144-1511980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/96957/96037Copyright (c) 2015 Clinicsinfo:eu-repo/semantics/openAccessLima, Gisele Negro Maganhin, Carla Cristina Simões, Ricardo Santos Baracat, Maria Cândida Pinheiro Sasso, Gisela Rodrigues da Silva Fuchs, Luiz Fernando Portugal Simões, Manuel de Jesus Baracat, Edmund Chada Soares Júnior, José Maria 2015-03-27T19:09:41Zoai:revistas.usp.br:article/96957Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2015-03-27T19:09:41Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
title |
Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
spellingShingle |
Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation Lima, Gisele Negro |
title_short |
Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
title_full |
Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
title_fullStr |
Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
title_full_unstemmed |
Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
title_sort |
Steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation |
author |
Lima, Gisele Negro |
author_facet |
Lima, Gisele Negro Maganhin, Carla Cristina Simões, Ricardo Santos Baracat, Maria Cândida Pinheiro Sasso, Gisela Rodrigues da Silva Fuchs, Luiz Fernando Portugal Simões, Manuel de Jesus Baracat, Edmund Chada Soares Júnior, José Maria |
author_role |
author |
author2 |
Maganhin, Carla Cristina Simões, Ricardo Santos Baracat, Maria Cândida Pinheiro Sasso, Gisela Rodrigues da Silva Fuchs, Luiz Fernando Portugal Simões, Manuel de Jesus Baracat, Edmund Chada Soares Júnior, José Maria |
author2_role |
author author author author author author author author |
dc.contributor.author.fl_str_mv |
Lima, Gisele Negro Maganhin, Carla Cristina Simões, Ricardo Santos Baracat, Maria Cândida Pinheiro Sasso, Gisela Rodrigues da Silva Fuchs, Luiz Fernando Portugal Simões, Manuel de Jesus Baracat, Edmund Chada Soares Júnior, José Maria |
description |
OBJECTIVE: To analyze steroidogenesis-related gene expression in the rat ovary exposed to melatonin supplementation. METHODS: Thirty-two virgin adult female rats were randomized to two groups as follows: the control group GI received vehicle and the experimental group GII received melatonin supplementation (10 µg/night per animal) for 60 consecutive days. After the treatment, animals were anesthetized and the collected ovaries were immediately placed in liquid nitrogen for complementary deoxyribonucleic acid microarray analyses. A GeneChip¯ Kit Rat Genome 230 2.0 Affymetrix Array was used for gene analysis and the experiment was repeated three times for each group. The results were normalized with the GeneChip¯ Operating Software program and confirmed through analysis with the secondary deoxyribonucleic acid-Chip Analyzer (dChip) software. The data were confirmed by real-time reverse transcription polymerase chain reaction analysis. Genes related to ovarian function were further confirmed by immunohistochemistry. RESULTS: We found the upregulation of the type 9 adenylate cyclase and inhibin beta B genes and the downregulation of the cyclic adenosine monophosphate response element modulator and cytochrome P450 family 17a1 genes in the ovarian tissue of GII compared to those of the control group. CONCLUSION: Our data suggest that melatonin supplementation decreases gene expression of cyclic adenosine monophosphate, which changes ovarian steroidogenesis. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/96957 10.6061/clinics/2015(02)12 |
url |
https://www.revistas.usp.br/clinics/article/view/96957 |
identifier_str_mv |
10.6061/clinics/2015(02)12 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/96957/96037 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 70 No. 2 (2015); 144-151 Clinics; v. 70 n. 2 (2015); 144-151 Clinics; Vol. 70 Núm. 2 (2015); 144-151 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222761712353280 |