Anaerobic bacteria in the intestinal microbiota of Brazilian children
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/130845 |
Resumo: | OBJECTIVE: Changes in the neonatal gut environment allow for the colonization of the mucin layer and lumen by anaerobic bacteria. The aim of the present study was to evaluate Bifidobacterium, Lactobacillus and Lactococcus colonization through the first year of life in a group of 12 Brazilian infants and to correlate these data with the levels of Escherichia coli. The presence of anaerobic members of the adult intestinal microbiota, including Eubacterium limosum and Faecalibacterium prausnitzii, was also evaluated. METHODS: Fecal samples were collected during the first year of life, and 16S rRNA from anaerobic and facultative bacteria was detected by real-time PCR. RESULTS: Bifidobacterium was present at the highest levels at all of the studied time points, followed by E. coli and Lactobacillus. E. limosum was rarely detected, and F. prausnitzii was detected only in the samples from the latest time points. CONCLUSION: These results are consistent with reports throughout the world on the community structure of the intestinal microbiota in infants fed a milk diet. Our findings also provide evidence for the influence of the environment on intestinal colonization due to the high abundance of E. coli. The presence of important anaerobic genera was observed in Brazilian infants living at a low socioeconomic level, a result that has already been well established for infants living in developed countries. |
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Clinics |
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Anaerobic bacteria in the intestinal microbiota of Brazilian childrenAnaerobic BacteriaIntestinal MicrobiotaBrazilian InfantsReal-Time PCROBJECTIVE: Changes in the neonatal gut environment allow for the colonization of the mucin layer and lumen by anaerobic bacteria. The aim of the present study was to evaluate Bifidobacterium, Lactobacillus and Lactococcus colonization through the first year of life in a group of 12 Brazilian infants and to correlate these data with the levels of Escherichia coli. The presence of anaerobic members of the adult intestinal microbiota, including Eubacterium limosum and Faecalibacterium prausnitzii, was also evaluated. METHODS: Fecal samples were collected during the first year of life, and 16S rRNA from anaerobic and facultative bacteria was detected by real-time PCR. RESULTS: Bifidobacterium was present at the highest levels at all of the studied time points, followed by E. coli and Lactobacillus. E. limosum was rarely detected, and F. prausnitzii was detected only in the samples from the latest time points. CONCLUSION: These results are consistent with reports throughout the world on the community structure of the intestinal microbiota in infants fed a milk diet. Our findings also provide evidence for the influence of the environment on intestinal colonization due to the high abundance of E. coli. The presence of important anaerobic genera was observed in Brazilian infants living at a low socioeconomic level, a result that has already been well established for infants living in developed countries.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2017-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/13084510.6061/clinics/2017(03)05Clinics; Vol. 72 No. 3 (2017); 154-160Clinics; v. 72 n. 3 (2017); 154-160Clinics; Vol. 72 Núm. 3 (2017); 154-1601980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/130845/127284Copyright (c) 2017 Clinicsinfo:eu-repo/semantics/openAccessTalarico, Silvia T.Santos, Florenza E.Brandt, Katia GaleãoMartinez, Marina B.Taddei, Carla R.2017-04-10T16:00:51Zoai:revistas.usp.br:article/130845Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2017-04-10T16:00:51Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Anaerobic bacteria in the intestinal microbiota of Brazilian children |
title |
Anaerobic bacteria in the intestinal microbiota of Brazilian children |
spellingShingle |
Anaerobic bacteria in the intestinal microbiota of Brazilian children Talarico, Silvia T. Anaerobic Bacteria Intestinal Microbiota Brazilian Infants Real-Time PCR |
title_short |
Anaerobic bacteria in the intestinal microbiota of Brazilian children |
title_full |
Anaerobic bacteria in the intestinal microbiota of Brazilian children |
title_fullStr |
Anaerobic bacteria in the intestinal microbiota of Brazilian children |
title_full_unstemmed |
Anaerobic bacteria in the intestinal microbiota of Brazilian children |
title_sort |
Anaerobic bacteria in the intestinal microbiota of Brazilian children |
author |
Talarico, Silvia T. |
author_facet |
Talarico, Silvia T. Santos, Florenza E. Brandt, Katia Galeão Martinez, Marina B. Taddei, Carla R. |
author_role |
author |
author2 |
Santos, Florenza E. Brandt, Katia Galeão Martinez, Marina B. Taddei, Carla R. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Talarico, Silvia T. Santos, Florenza E. Brandt, Katia Galeão Martinez, Marina B. Taddei, Carla R. |
dc.subject.por.fl_str_mv |
Anaerobic Bacteria Intestinal Microbiota Brazilian Infants Real-Time PCR |
topic |
Anaerobic Bacteria Intestinal Microbiota Brazilian Infants Real-Time PCR |
description |
OBJECTIVE: Changes in the neonatal gut environment allow for the colonization of the mucin layer and lumen by anaerobic bacteria. The aim of the present study was to evaluate Bifidobacterium, Lactobacillus and Lactococcus colonization through the first year of life in a group of 12 Brazilian infants and to correlate these data with the levels of Escherichia coli. The presence of anaerobic members of the adult intestinal microbiota, including Eubacterium limosum and Faecalibacterium prausnitzii, was also evaluated. METHODS: Fecal samples were collected during the first year of life, and 16S rRNA from anaerobic and facultative bacteria was detected by real-time PCR. RESULTS: Bifidobacterium was present at the highest levels at all of the studied time points, followed by E. coli and Lactobacillus. E. limosum was rarely detected, and F. prausnitzii was detected only in the samples from the latest time points. CONCLUSION: These results are consistent with reports throughout the world on the community structure of the intestinal microbiota in infants fed a milk diet. Our findings also provide evidence for the influence of the environment on intestinal colonization due to the high abundance of E. coli. The presence of important anaerobic genera was observed in Brazilian infants living at a low socioeconomic level, a result that has already been well established for infants living in developed countries. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/130845 10.6061/clinics/2017(03)05 |
url |
https://www.revistas.usp.br/clinics/article/view/130845 |
identifier_str_mv |
10.6061/clinics/2017(03)05 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/130845/127284 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 72 No. 3 (2017); 154-160 Clinics; v. 72 n. 3 (2017); 154-160 Clinics; Vol. 72 Núm. 3 (2017); 154-160 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222763136319488 |