Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma

Detalhes bibliográficos
Autor(a) principal: Miura, Flávio Key
Data de Publicação: 2010
Outros Autores: Alves, Maria Jose Ferreira, Rocha, Mussya Cisotto, Silva, Roseli da, Oba-Shinjo, Sueli Mieko, Marie, Suely Kazue Nagahashi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/18388
Resumo: INTRODUCTION: Astrocytic gliomas are the most common intracranial central nervous system neoplasias, accounting for about 60% of all primary central nervous system tumors. Despite advances in the treatment of gliomas, no effective therapeutic approach is yet available; hence, the search for a more realistic model to generate more effective therapies is essential. OBJECTIVE: To develop an experimental malignant astrocytoma model with the characteristics of the human tumor. METHOD: Primary cells from subcutaneous xenograft tumors produced with malignant astrocytoma U87MG cells were inoculated intracerebrally by stereotaxis into immunosuppressed (athymic) Rowett rats. RESULTS: All four injected animals developed non-infiltrative tumors, although other glioblastoma characteristics, such as necrosis, pseudopalisading cells and intense mitotic activity, were observed. CONCLUSION: A malignant astrocytoma intracerebral xenograft model with poorly invasive behavior was achieved in athymic Rowett rats. Tumor invasiveness in an experimental animal model may depend on a combination of several factors, including the cell line used to induce tumor formation, the rat strains and the status of the animal's immune system.
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spelling Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma Brain tumorExperimental modelAthymic Rowett ratsU87MG cells INTRODUCTION: Astrocytic gliomas are the most common intracranial central nervous system neoplasias, accounting for about 60% of all primary central nervous system tumors. Despite advances in the treatment of gliomas, no effective therapeutic approach is yet available; hence, the search for a more realistic model to generate more effective therapies is essential. OBJECTIVE: To develop an experimental malignant astrocytoma model with the characteristics of the human tumor. METHOD: Primary cells from subcutaneous xenograft tumors produced with malignant astrocytoma U87MG cells were inoculated intracerebrally by stereotaxis into immunosuppressed (athymic) Rowett rats. RESULTS: All four injected animals developed non-infiltrative tumors, although other glioblastoma characteristics, such as necrosis, pseudopalisading cells and intense mitotic activity, were observed. CONCLUSION: A malignant astrocytoma intracerebral xenograft model with poorly invasive behavior was achieved in athymic Rowett rats. Tumor invasiveness in an experimental animal model may depend on a combination of several factors, including the cell line used to induce tumor formation, the rat strains and the status of the animal's immune system. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2010-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1838810.1590/S1807-59322010000300011Clinics; Vol. 65 No. 3 (2010); 305-309 Clinics; v. 65 n. 3 (2010); 305-309 Clinics; Vol. 65 Núm. 3 (2010); 305-309 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/18388/20451Miura, Flávio KeyAlves, Maria Jose FerreiraRocha, Mussya CisottoSilva, Roseli daOba-Shinjo, Sueli MiekoMarie, Suely Kazue Nagahashiinfo:eu-repo/semantics/openAccess2012-05-23T11:19:25Zoai:revistas.usp.br:article/18388Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T11:19:25Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma
title Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma
spellingShingle Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma
Miura, Flávio Key
Brain tumor
Experimental model
Athymic Rowett rats
U87MG cells
title_short Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma
title_full Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma
title_fullStr Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma
title_full_unstemmed Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma
title_sort Xenograft transplantation of human malignant astrocytoma cells into immunodeficient rats: an experimental model of glioblastoma
author Miura, Flávio Key
author_facet Miura, Flávio Key
Alves, Maria Jose Ferreira
Rocha, Mussya Cisotto
Silva, Roseli da
Oba-Shinjo, Sueli Mieko
Marie, Suely Kazue Nagahashi
author_role author
author2 Alves, Maria Jose Ferreira
Rocha, Mussya Cisotto
Silva, Roseli da
Oba-Shinjo, Sueli Mieko
Marie, Suely Kazue Nagahashi
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Miura, Flávio Key
Alves, Maria Jose Ferreira
Rocha, Mussya Cisotto
Silva, Roseli da
Oba-Shinjo, Sueli Mieko
Marie, Suely Kazue Nagahashi
dc.subject.por.fl_str_mv Brain tumor
Experimental model
Athymic Rowett rats
U87MG cells
topic Brain tumor
Experimental model
Athymic Rowett rats
U87MG cells
description INTRODUCTION: Astrocytic gliomas are the most common intracranial central nervous system neoplasias, accounting for about 60% of all primary central nervous system tumors. Despite advances in the treatment of gliomas, no effective therapeutic approach is yet available; hence, the search for a more realistic model to generate more effective therapies is essential. OBJECTIVE: To develop an experimental malignant astrocytoma model with the characteristics of the human tumor. METHOD: Primary cells from subcutaneous xenograft tumors produced with malignant astrocytoma U87MG cells were inoculated intracerebrally by stereotaxis into immunosuppressed (athymic) Rowett rats. RESULTS: All four injected animals developed non-infiltrative tumors, although other glioblastoma characteristics, such as necrosis, pseudopalisading cells and intense mitotic activity, were observed. CONCLUSION: A malignant astrocytoma intracerebral xenograft model with poorly invasive behavior was achieved in athymic Rowett rats. Tumor invasiveness in an experimental animal model may depend on a combination of several factors, including the cell line used to induce tumor formation, the rat strains and the status of the animal's immune system.
publishDate 2010
dc.date.none.fl_str_mv 2010-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/18388
10.1590/S1807-59322010000300011
url https://www.revistas.usp.br/clinics/article/view/18388
identifier_str_mv 10.1590/S1807-59322010000300011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/18388/20451
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 65 No. 3 (2010); 305-309
Clinics; v. 65 n. 3 (2010); 305-309
Clinics; Vol. 65 Núm. 3 (2010); 305-309
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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