Hypertonic saline and reduced peroxynitrite formation in experimental pancreatitis
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/19240 |
Resumo: | OBJECTIVES: In this study, we tested the hypothesis that hypertonic saline exerts anti-inflammatory effects by modulating hepatic oxidative stress in pancreatitis. INTRODUCTION: The incidence of hepatic injury is related to severe pancreatitis, and hypertonic saline reduces pancreatic injury and mortality in pancreatitis. METHODS: Wistar rats were divided into four groups: control (not subjected to treatment), untreated pancreatitis (NT, pancreatitis induced by a retrograde transduodenal infusion of 2.5% sodium taurocholate into the pancreatic duct with no further treatment administered), pancreatitis with normal saline (NS, pancreatitis induced as described above and followed by resuscitation with 0.9% NaCl), and pancreatitis with hypertonic saline (HS, pancreatitis induced as described above and followed by resuscitation with 7.5% NaCl). At 4, 12, and 24 h after pancreatitis induction, liver levels of inducible nitric oxide synthase (iNOS), heat-shock protein 70, nitrotyrosine (formation of peroxynitrite), nitrite/nitrate production, lipid peroxidation, and alanine aminotransferase (ALT) release were determined. RESULTS: Twelve hours after pancreatitis induction, animals in the HS group presented significantly lower iNOS expression (P |
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Hypertonic saline and reduced peroxynitrite formation in experimental pancreatitis Liver injuryOxidative stressHypertonicityLipid PeroxidationNitric Oxide OBJECTIVES: In this study, we tested the hypothesis that hypertonic saline exerts anti-inflammatory effects by modulating hepatic oxidative stress in pancreatitis. INTRODUCTION: The incidence of hepatic injury is related to severe pancreatitis, and hypertonic saline reduces pancreatic injury and mortality in pancreatitis. METHODS: Wistar rats were divided into four groups: control (not subjected to treatment), untreated pancreatitis (NT, pancreatitis induced by a retrograde transduodenal infusion of 2.5% sodium taurocholate into the pancreatic duct with no further treatment administered), pancreatitis with normal saline (NS, pancreatitis induced as described above and followed by resuscitation with 0.9% NaCl), and pancreatitis with hypertonic saline (HS, pancreatitis induced as described above and followed by resuscitation with 7.5% NaCl). At 4, 12, and 24 h after pancreatitis induction, liver levels of inducible nitric oxide synthase (iNOS), heat-shock protein 70, nitrotyrosine (formation of peroxynitrite), nitrite/nitrate production, lipid peroxidation, and alanine aminotransferase (ALT) release were determined. RESULTS: Twelve hours after pancreatitis induction, animals in the HS group presented significantly lower iNOS expression (PHospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1924010.1590/S1807-59322011000300019Clinics; Vol. 66 No. 3 (2011); 469-476 Clinics; v. 66 n. 3 (2011); 469-476 Clinics; Vol. 66 Núm. 3 (2011); 469-476 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/19240/21303Rios, Ester Correia SarmentoMoretti, Ana SoaresVelasco, Irineu TadeuSouza, Heraldo Possolo deAbatepaulo, FatimaSoriano, Franciscoinfo:eu-repo/semantics/openAccess2012-05-23T16:29:25Zoai:revistas.usp.br:article/19240Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T16:29:25Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Hypertonic saline and reduced peroxynitrite formation in experimental pancreatitis |
title |
Hypertonic saline and reduced peroxynitrite formation in experimental pancreatitis |
spellingShingle |
Hypertonic saline and reduced peroxynitrite formation in experimental pancreatitis Rios, Ester Correia Sarmento Liver injury Oxidative stress Hypertonicity Lipid Peroxidation Nitric Oxide |
title_short |
Hypertonic saline and reduced peroxynitrite formation in experimental pancreatitis |
title_full |
Hypertonic saline and reduced peroxynitrite formation in experimental pancreatitis |
title_fullStr |
Hypertonic saline and reduced peroxynitrite formation in experimental pancreatitis |
title_full_unstemmed |
Hypertonic saline and reduced peroxynitrite formation in experimental pancreatitis |
title_sort |
Hypertonic saline and reduced peroxynitrite formation in experimental pancreatitis |
author |
Rios, Ester Correia Sarmento |
author_facet |
Rios, Ester Correia Sarmento Moretti, Ana Soares Velasco, Irineu Tadeu Souza, Heraldo Possolo de Abatepaulo, Fatima Soriano, Francisco |
author_role |
author |
author2 |
Moretti, Ana Soares Velasco, Irineu Tadeu Souza, Heraldo Possolo de Abatepaulo, Fatima Soriano, Francisco |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rios, Ester Correia Sarmento Moretti, Ana Soares Velasco, Irineu Tadeu Souza, Heraldo Possolo de Abatepaulo, Fatima Soriano, Francisco |
dc.subject.por.fl_str_mv |
Liver injury Oxidative stress Hypertonicity Lipid Peroxidation Nitric Oxide |
topic |
Liver injury Oxidative stress Hypertonicity Lipid Peroxidation Nitric Oxide |
description |
OBJECTIVES: In this study, we tested the hypothesis that hypertonic saline exerts anti-inflammatory effects by modulating hepatic oxidative stress in pancreatitis. INTRODUCTION: The incidence of hepatic injury is related to severe pancreatitis, and hypertonic saline reduces pancreatic injury and mortality in pancreatitis. METHODS: Wistar rats were divided into four groups: control (not subjected to treatment), untreated pancreatitis (NT, pancreatitis induced by a retrograde transduodenal infusion of 2.5% sodium taurocholate into the pancreatic duct with no further treatment administered), pancreatitis with normal saline (NS, pancreatitis induced as described above and followed by resuscitation with 0.9% NaCl), and pancreatitis with hypertonic saline (HS, pancreatitis induced as described above and followed by resuscitation with 7.5% NaCl). At 4, 12, and 24 h after pancreatitis induction, liver levels of inducible nitric oxide synthase (iNOS), heat-shock protein 70, nitrotyrosine (formation of peroxynitrite), nitrite/nitrate production, lipid peroxidation, and alanine aminotransferase (ALT) release were determined. RESULTS: Twelve hours after pancreatitis induction, animals in the HS group presented significantly lower iNOS expression (P |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19240 10.1590/S1807-59322011000300019 |
url |
https://www.revistas.usp.br/clinics/article/view/19240 |
identifier_str_mv |
10.1590/S1807-59322011000300019 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19240/21303 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 66 No. 3 (2011); 469-476 Clinics; v. 66 n. 3 (2011); 469-476 Clinics; Vol. 66 Núm. 3 (2011); 469-476 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222756292263936 |