Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/154696 |
Resumo: | OBJECTIVES: The objective of the present study was to evaluate the protective effect of pre-conditioning treatment with laser light on hepatic injury in rats submitted to partial ischemia using mitochondrial function and liver fatty acid binding protein as markers. METHODS: Rats were divided into four groups (n=5): 1) Control, 2) Control + Laser, 3) Partial Ischemia and 4) Partial Ischemia + Laser. Ischemia was induced by clamping the hepatic pedicle of the left and middle lobes of the liver for 60 minutes. Laser light at 660 nm was applied to the liver immediately prior to the induction of ischemia at 22.5 J/cm2 , with 30 seconds of illumination at five individual points. The animals were sacrificed after 30 minutes of reperfusion. Blood and liver tissues were collected for analysis of mitochondrial function, determination of malondialdehyde and analysis of fatty acid binding protein expression by Western blot. RESULTS: Mitochondrial function decreased in the Partial Ischemia group, especially during adenosine diphosphate-activated respiration (state 3), and the expression of fatty acid binding protein was also reduced. The application of laser light prevented bioenergetic changes and restored the expression of fatty acid binding protein. CONCLUSION: Prophylactic application of laser light to the livers of rats submitted to partial ischemia was found to have a protective effect in the liver, with normalization of both mitochondrial function and fatty acid binding protein tissue expression. |
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network_name_str |
Clinics |
repository_id_str |
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Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemiaIschemiaReperfusion InjuryLaser TherapyL-FABPOBJECTIVES: The objective of the present study was to evaluate the protective effect of pre-conditioning treatment with laser light on hepatic injury in rats submitted to partial ischemia using mitochondrial function and liver fatty acid binding protein as markers. METHODS: Rats were divided into four groups (n=5): 1) Control, 2) Control + Laser, 3) Partial Ischemia and 4) Partial Ischemia + Laser. Ischemia was induced by clamping the hepatic pedicle of the left and middle lobes of the liver for 60 minutes. Laser light at 660 nm was applied to the liver immediately prior to the induction of ischemia at 22.5 J/cm2 , with 30 seconds of illumination at five individual points. The animals were sacrificed after 30 minutes of reperfusion. Blood and liver tissues were collected for analysis of mitochondrial function, determination of malondialdehyde and analysis of fatty acid binding protein expression by Western blot. RESULTS: Mitochondrial function decreased in the Partial Ischemia group, especially during adenosine diphosphate-activated respiration (state 3), and the expression of fatty acid binding protein was also reduced. The application of laser light prevented bioenergetic changes and restored the expression of fatty acid binding protein. CONCLUSION: Prophylactic application of laser light to the livers of rats submitted to partial ischemia was found to have a protective effect in the liver, with normalization of both mitochondrial function and fatty acid binding protein tissue expression.Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2019-02-13info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/15469610.6061/clinics/2018/e113Clinics; Vol. 73 (2018); e113Clinics; v. 73 (2018); e113Clinics; Vol. 73 (2018); e1131980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/154696/150752Copyright (c) 2019 Clinicsinfo:eu-repo/semantics/openAccessVilalva, Kelvin HenriqueFigueira, Rebeca LopesSilveira, MarinaGraf, CatarinaGonçalves, Frances LanhellasSbragia, LourençoGomes, Maria CecíliaMumic, FabricíaVollet-Filho, Jose DirceuBagnato, Vanderlei SalvadorD’Albuquerque, Luiz Augusto CarneiroCastro-e-Silva, Orlando2019-05-14T11:48:50Zoai:revistas.usp.br:article/154696Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2019-05-14T11:48:50Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia |
title |
Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia |
spellingShingle |
Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia Vilalva, Kelvin Henrique Ischemia Reperfusion Injury Laser Therapy L-FABP |
title_short |
Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia |
title_full |
Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia |
title_fullStr |
Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia |
title_full_unstemmed |
Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia |
title_sort |
Prophylactic application of laser light restores L-FABP expression in the livers of rats submitted to partial ischemia |
author |
Vilalva, Kelvin Henrique |
author_facet |
Vilalva, Kelvin Henrique Figueira, Rebeca Lopes Silveira, Marina Graf, Catarina Gonçalves, Frances Lanhellas Sbragia, Lourenço Gomes, Maria Cecília Mumic, Fabricía Vollet-Filho, Jose Dirceu Bagnato, Vanderlei Salvador D’Albuquerque, Luiz Augusto Carneiro Castro-e-Silva, Orlando |
author_role |
author |
author2 |
Figueira, Rebeca Lopes Silveira, Marina Graf, Catarina Gonçalves, Frances Lanhellas Sbragia, Lourenço Gomes, Maria Cecília Mumic, Fabricía Vollet-Filho, Jose Dirceu Bagnato, Vanderlei Salvador D’Albuquerque, Luiz Augusto Carneiro Castro-e-Silva, Orlando |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Vilalva, Kelvin Henrique Figueira, Rebeca Lopes Silveira, Marina Graf, Catarina Gonçalves, Frances Lanhellas Sbragia, Lourenço Gomes, Maria Cecília Mumic, Fabricía Vollet-Filho, Jose Dirceu Bagnato, Vanderlei Salvador D’Albuquerque, Luiz Augusto Carneiro Castro-e-Silva, Orlando |
dc.subject.por.fl_str_mv |
Ischemia Reperfusion Injury Laser Therapy L-FABP |
topic |
Ischemia Reperfusion Injury Laser Therapy L-FABP |
description |
OBJECTIVES: The objective of the present study was to evaluate the protective effect of pre-conditioning treatment with laser light on hepatic injury in rats submitted to partial ischemia using mitochondrial function and liver fatty acid binding protein as markers. METHODS: Rats were divided into four groups (n=5): 1) Control, 2) Control + Laser, 3) Partial Ischemia and 4) Partial Ischemia + Laser. Ischemia was induced by clamping the hepatic pedicle of the left and middle lobes of the liver for 60 minutes. Laser light at 660 nm was applied to the liver immediately prior to the induction of ischemia at 22.5 J/cm2 , with 30 seconds of illumination at five individual points. The animals were sacrificed after 30 minutes of reperfusion. Blood and liver tissues were collected for analysis of mitochondrial function, determination of malondialdehyde and analysis of fatty acid binding protein expression by Western blot. RESULTS: Mitochondrial function decreased in the Partial Ischemia group, especially during adenosine diphosphate-activated respiration (state 3), and the expression of fatty acid binding protein was also reduced. The application of laser light prevented bioenergetic changes and restored the expression of fatty acid binding protein. CONCLUSION: Prophylactic application of laser light to the livers of rats submitted to partial ischemia was found to have a protective effect in the liver, with normalization of both mitochondrial function and fatty acid binding protein tissue expression. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-02-13 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/154696 10.6061/clinics/2018/e113 |
url |
https://www.revistas.usp.br/clinics/article/view/154696 |
identifier_str_mv |
10.6061/clinics/2018/e113 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/154696/150752 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 73 (2018); e113 Clinics; v. 73 (2018); e113 Clinics; Vol. 73 (2018); e113 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222763717230592 |