FOXP3 variants are independently associated with transforming growth factor Β1 plasma levels in female patients with inflammatory bowel disease

• Autor(a) principal: Inoue, Cláudia Junko
• Data de Publicação: 2022
• Outros Autores: Flauzino, Tamires, Gonçalves, Beatriz Piantoni, Paula, Jaqueline Costa Castardo de, Galvão, Talita Cristina, Miyazaki, Paula Kikuchi, Alcantara, Camila Cataldi de, Westmore, Lucilene Rosa e Silva, Lozovoy, Marcell Alysson Batisti, Reiche, Edna Maria Vissoci, Simão, Andréa Name Colado
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Clinics
• Texto Completo: https://www.revistas.usp.br/clinics/article/view/213522
• topic: Inflammatory bowel diseases; Ulcerative colitis; rohn's disease; Forkhead box protein 3 genetic variants; Transforming growth Factor β1

Objective: The aim of this study was to evaluate the association of -924 G>A (rs2232365) and -3279 C>A (rs3761548) FOXP3 variants with IBD susceptibility, clinical and endoscopic activity, and IL-10 and TGF-β1 plasma levels. Method: The study included 110 IBD female patients, 60 with Ulcerative Colitis (UC) and 50 with Crohn's Disease (CD), and 154 female controls. FOXP3 variants were determined with Polymerase Chain Reaction-Restriction Fragment Length Polymorphism (PCR-RFLP). Plasma levels of IL-10 and TGF-β1 were determined using immunofluorimetric assay. Results: AA genotype of rs2232365 and rs3761548 was associated with CD (OR = 3.147, 95% CI 1.015–9.758, p = 0.047) and UC (OR = 3.221, 95% CI 1.050–9.876, p = 0.041) susceptibility, respectively. However, were not associated with TGF-β1 and IL-10 levels, and endoscopic/clinical activity disease. GAGA haplotype was associated with IBD (OR = 4.003, 95% CI 1.100–14.56, p = 0.035) and UC susceptibility (OR = 6.107, 95% CI 1.609–23.18, p = 0.008). In addition, IBD patients with the GAGA haplotype had lower TGF-β1 levels (p = 0.041). Moreover, G/C haplotype (dominant model) had a protective effect of 60% in CD susceptibility and lower Endoscopic Severity Index. Conclusions: These results suggest that FOXP3 variants could exert a role in the Treg, which could be one of the factors involved in the susceptibility and pathogenesis of IBD.