Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/18074 |
Resumo: | INTRODUCTION: Elastic and collagen fiber deposition increases throughout normal lung development, and this fiber network significantly changes when development of the lung is disturbed. In preterm rats and lambs, prolonged hyperoxic exposure is associated with impaired alveolization and causes significant changes in the deposition and structure of elastic fibers. OBJECTIVES: To evaluate the effects of hyperoxic exposure on elastic and collagen fiber deposition in the lung interstitial matrix and in alveolarization in preterm rabbits. METHODS: After c-section, 28-day preterm New-Zealand-White rabbits were randomized into 2 study groups, according to the oxygen exposure, namely: Room air (oxygen = 21%) or Oxygen (oxygen >; 95%). The animals were killed on day 11 and their lungs were analyzed for the alveolar size (Lm), the internal surface area (ISA), the alveoli number, and the density and distribution of collagen and elastic fibers. RESULTS: An increase in the Lm and a decrease in the alveoli number were observed among rabbits that were exposed to hyperoxia with no differences regarding the ISA. No difference in the density of elastic fibers was observed after oxygen exposure, however there were fewer collagen fibers and an evident disorganization of fiber deposition. DISCUSSION: This model reproduces anatomo-pathological injuries representing the arrest of normal alveolar development and lung architecture disorganization by just a prolonged exposition to oxygen. CONCLUSIONS: In the preterm rabbit, prolonged oxygen exposure impaired alveolization and also lowered the proportion of collagen fibers, with an evident fiber network disorganization. |
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Clinics |
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Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits OxygenAnimal modelLung fibersBronchopulmonary dysplasiaLung injury INTRODUCTION: Elastic and collagen fiber deposition increases throughout normal lung development, and this fiber network significantly changes when development of the lung is disturbed. In preterm rats and lambs, prolonged hyperoxic exposure is associated with impaired alveolization and causes significant changes in the deposition and structure of elastic fibers. OBJECTIVES: To evaluate the effects of hyperoxic exposure on elastic and collagen fiber deposition in the lung interstitial matrix and in alveolarization in preterm rabbits. METHODS: After c-section, 28-day preterm New-Zealand-White rabbits were randomized into 2 study groups, according to the oxygen exposure, namely: Room air (oxygen = 21%) or Oxygen (oxygen >; 95%). The animals were killed on day 11 and their lungs were analyzed for the alveolar size (Lm), the internal surface area (ISA), the alveoli number, and the density and distribution of collagen and elastic fibers. RESULTS: An increase in the Lm and a decrease in the alveoli number were observed among rabbits that were exposed to hyperoxia with no differences regarding the ISA. No difference in the density of elastic fibers was observed after oxygen exposure, however there were fewer collagen fibers and an evident disorganization of fiber deposition. DISCUSSION: This model reproduces anatomo-pathological injuries representing the arrest of normal alveolar development and lung architecture disorganization by just a prolonged exposition to oxygen. CONCLUSIONS: In the preterm rabbit, prolonged oxygen exposure impaired alveolization and also lowered the proportion of collagen fibers, with an evident fiber network disorganization. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2009-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1807410.1590/S1807-59322009001100010Clinics; Vol. 64 No. 11 (2009); 1099-1104 Clinics; v. 64 n. 11 (2009); 1099-1104 Clinics; Vol. 64 Núm. 11 (2009); 1099-1104 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/18074/20138Mascaretti, Renata SumanMataloun, Marta Maria Galli BozzoDolhnikoff, MarisaRebello, Celso Mourainfo:eu-repo/semantics/openAccess2012-05-22T18:54:31Zoai:revistas.usp.br:article/18074Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-22T18:54:31Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits |
title |
Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits |
spellingShingle |
Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits Mascaretti, Renata Suman Oxygen Animal model Lung fibers Bronchopulmonary dysplasia Lung injury |
title_short |
Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits |
title_full |
Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits |
title_fullStr |
Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits |
title_full_unstemmed |
Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits |
title_sort |
Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits |
author |
Mascaretti, Renata Suman |
author_facet |
Mascaretti, Renata Suman Mataloun, Marta Maria Galli Bozzo Dolhnikoff, Marisa Rebello, Celso Moura |
author_role |
author |
author2 |
Mataloun, Marta Maria Galli Bozzo Dolhnikoff, Marisa Rebello, Celso Moura |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Mascaretti, Renata Suman Mataloun, Marta Maria Galli Bozzo Dolhnikoff, Marisa Rebello, Celso Moura |
dc.subject.por.fl_str_mv |
Oxygen Animal model Lung fibers Bronchopulmonary dysplasia Lung injury |
topic |
Oxygen Animal model Lung fibers Bronchopulmonary dysplasia Lung injury |
description |
INTRODUCTION: Elastic and collagen fiber deposition increases throughout normal lung development, and this fiber network significantly changes when development of the lung is disturbed. In preterm rats and lambs, prolonged hyperoxic exposure is associated with impaired alveolization and causes significant changes in the deposition and structure of elastic fibers. OBJECTIVES: To evaluate the effects of hyperoxic exposure on elastic and collagen fiber deposition in the lung interstitial matrix and in alveolarization in preterm rabbits. METHODS: After c-section, 28-day preterm New-Zealand-White rabbits were randomized into 2 study groups, according to the oxygen exposure, namely: Room air (oxygen = 21%) or Oxygen (oxygen >; 95%). The animals were killed on day 11 and their lungs were analyzed for the alveolar size (Lm), the internal surface area (ISA), the alveoli number, and the density and distribution of collagen and elastic fibers. RESULTS: An increase in the Lm and a decrease in the alveoli number were observed among rabbits that were exposed to hyperoxia with no differences regarding the ISA. No difference in the density of elastic fibers was observed after oxygen exposure, however there were fewer collagen fibers and an evident disorganization of fiber deposition. DISCUSSION: This model reproduces anatomo-pathological injuries representing the arrest of normal alveolar development and lung architecture disorganization by just a prolonged exposition to oxygen. CONCLUSIONS: In the preterm rabbit, prolonged oxygen exposure impaired alveolization and also lowered the proportion of collagen fibers, with an evident fiber network disorganization. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/18074 10.1590/S1807-59322009001100010 |
url |
https://www.revistas.usp.br/clinics/article/view/18074 |
identifier_str_mv |
10.1590/S1807-59322009001100010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/18074/20138 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 64 No. 11 (2009); 1099-1104 Clinics; v. 64 n. 11 (2009); 1099-1104 Clinics; Vol. 64 Núm. 11 (2009); 1099-1104 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222754809577472 |