Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits

Detalhes bibliográficos
Autor(a) principal: Mascaretti, Renata Suman
Data de Publicação: 2009
Outros Autores: Mataloun, Marta Maria Galli Bozzo, Dolhnikoff, Marisa, Rebello, Celso Moura
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/18074
Resumo: INTRODUCTION: Elastic and collagen fiber deposition increases throughout normal lung development, and this fiber network significantly changes when development of the lung is disturbed. In preterm rats and lambs, prolonged hyperoxic exposure is associated with impaired alveolization and causes significant changes in the deposition and structure of elastic fibers. OBJECTIVES: To evaluate the effects of hyperoxic exposure on elastic and collagen fiber deposition in the lung interstitial matrix and in alveolarization in preterm rabbits. METHODS: After c-section, 28-day preterm New-Zealand-White rabbits were randomized into 2 study groups, according to the oxygen exposure, namely: Room air (oxygen = 21%) or Oxygen (oxygen >; 95%). The animals were killed on day 11 and their lungs were analyzed for the alveolar size (Lm), the internal surface area (ISA), the alveoli number, and the density and distribution of collagen and elastic fibers. RESULTS: An increase in the Lm and a decrease in the alveoli number were observed among rabbits that were exposed to hyperoxia with no differences regarding the ISA. No difference in the density of elastic fibers was observed after oxygen exposure, however there were fewer collagen fibers and an evident disorganization of fiber deposition. DISCUSSION: This model reproduces anatomo-pathological injuries representing the arrest of normal alveolar development and lung architecture disorganization by just a prolonged exposition to oxygen. CONCLUSIONS: In the preterm rabbit, prolonged oxygen exposure impaired alveolization and also lowered the proportion of collagen fibers, with an evident fiber network disorganization.
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spelling Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits OxygenAnimal modelLung fibersBronchopulmonary dysplasiaLung injury INTRODUCTION: Elastic and collagen fiber deposition increases throughout normal lung development, and this fiber network significantly changes when development of the lung is disturbed. In preterm rats and lambs, prolonged hyperoxic exposure is associated with impaired alveolization and causes significant changes in the deposition and structure of elastic fibers. OBJECTIVES: To evaluate the effects of hyperoxic exposure on elastic and collagen fiber deposition in the lung interstitial matrix and in alveolarization in preterm rabbits. METHODS: After c-section, 28-day preterm New-Zealand-White rabbits were randomized into 2 study groups, according to the oxygen exposure, namely: Room air (oxygen = 21%) or Oxygen (oxygen >; 95%). The animals were killed on day 11 and their lungs were analyzed for the alveolar size (Lm), the internal surface area (ISA), the alveoli number, and the density and distribution of collagen and elastic fibers. RESULTS: An increase in the Lm and a decrease in the alveoli number were observed among rabbits that were exposed to hyperoxia with no differences regarding the ISA. No difference in the density of elastic fibers was observed after oxygen exposure, however there were fewer collagen fibers and an evident disorganization of fiber deposition. DISCUSSION: This model reproduces anatomo-pathological injuries representing the arrest of normal alveolar development and lung architecture disorganization by just a prolonged exposition to oxygen. CONCLUSIONS: In the preterm rabbit, prolonged oxygen exposure impaired alveolization and also lowered the proportion of collagen fibers, with an evident fiber network disorganization. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2009-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1807410.1590/S1807-59322009001100010Clinics; Vol. 64 No. 11 (2009); 1099-1104 Clinics; v. 64 n. 11 (2009); 1099-1104 Clinics; Vol. 64 Núm. 11 (2009); 1099-1104 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/18074/20138Mascaretti, Renata SumanMataloun, Marta Maria Galli BozzoDolhnikoff, MarisaRebello, Celso Mourainfo:eu-repo/semantics/openAccess2012-05-22T18:54:31Zoai:revistas.usp.br:article/18074Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-22T18:54:31Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits
title Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits
spellingShingle Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits
Mascaretti, Renata Suman
Oxygen
Animal model
Lung fibers
Bronchopulmonary dysplasia
Lung injury
title_short Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits
title_full Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits
title_fullStr Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits
title_full_unstemmed Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits
title_sort Lung morphometry, collagen and elastin content: changes after hyperoxic exposure in preterm rabbits
author Mascaretti, Renata Suman
author_facet Mascaretti, Renata Suman
Mataloun, Marta Maria Galli Bozzo
Dolhnikoff, Marisa
Rebello, Celso Moura
author_role author
author2 Mataloun, Marta Maria Galli Bozzo
Dolhnikoff, Marisa
Rebello, Celso Moura
author2_role author
author
author
dc.contributor.author.fl_str_mv Mascaretti, Renata Suman
Mataloun, Marta Maria Galli Bozzo
Dolhnikoff, Marisa
Rebello, Celso Moura
dc.subject.por.fl_str_mv Oxygen
Animal model
Lung fibers
Bronchopulmonary dysplasia
Lung injury
topic Oxygen
Animal model
Lung fibers
Bronchopulmonary dysplasia
Lung injury
description INTRODUCTION: Elastic and collagen fiber deposition increases throughout normal lung development, and this fiber network significantly changes when development of the lung is disturbed. In preterm rats and lambs, prolonged hyperoxic exposure is associated with impaired alveolization and causes significant changes in the deposition and structure of elastic fibers. OBJECTIVES: To evaluate the effects of hyperoxic exposure on elastic and collagen fiber deposition in the lung interstitial matrix and in alveolarization in preterm rabbits. METHODS: After c-section, 28-day preterm New-Zealand-White rabbits were randomized into 2 study groups, according to the oxygen exposure, namely: Room air (oxygen = 21%) or Oxygen (oxygen >; 95%). The animals were killed on day 11 and their lungs were analyzed for the alveolar size (Lm), the internal surface area (ISA), the alveoli number, and the density and distribution of collagen and elastic fibers. RESULTS: An increase in the Lm and a decrease in the alveoli number were observed among rabbits that were exposed to hyperoxia with no differences regarding the ISA. No difference in the density of elastic fibers was observed after oxygen exposure, however there were fewer collagen fibers and an evident disorganization of fiber deposition. DISCUSSION: This model reproduces anatomo-pathological injuries representing the arrest of normal alveolar development and lung architecture disorganization by just a prolonged exposition to oxygen. CONCLUSIONS: In the preterm rabbit, prolonged oxygen exposure impaired alveolization and also lowered the proportion of collagen fibers, with an evident fiber network disorganization.
publishDate 2009
dc.date.none.fl_str_mv 2009-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/18074
10.1590/S1807-59322009001100010
url https://www.revistas.usp.br/clinics/article/view/18074
identifier_str_mv 10.1590/S1807-59322009001100010
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/18074/20138
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 64 No. 11 (2009); 1099-1104
Clinics; v. 64 n. 11 (2009); 1099-1104
Clinics; Vol. 64 Núm. 11 (2009); 1099-1104
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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