Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/103939 |
Resumo: | OBJECTIVE:To quantify the collagen fibers in the lacrimal gland of female mice with hyperprolactinemia.METHODS:Forty adult female mice were randomly divided into two groups with 20 animals each: nonpregnant control (CTR1, control group, 0.2 mL of saline solution) and nonpregnant experimental (HPRL1, experimental group, 200 µg/day metoclopramide). Treatments lasted for 50 consecutive days. On day 50, 10 females from each group (control and experimental) were euthanized in the proestrus phase; then, the blood was collected and the lacrimal glands were removed. Thereafter, the remaining females were placed with the mates and continued to receive treatment with saline solution or metoclopramide. On the 6th post-coital day, 10 pregnant females from the control group (CTR2) and 10 pregnant females from the experimental group (HPRL2) were euthanized, after which blood was collected and the lacrimal glands removed. The lacrimal glands were processed for morphological analyses and collagen quantification, and prolactin and sex steroid levels were measured in the blood samples. Data were statistically analyzed using an unpaired Student t test (p |
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oai:revistas.usp.br:article/103939 |
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Clinics |
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Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice OBJECTIVE:To quantify the collagen fibers in the lacrimal gland of female mice with hyperprolactinemia.METHODS:Forty adult female mice were randomly divided into two groups with 20 animals each: nonpregnant control (CTR1, control group, 0.2 mL of saline solution) and nonpregnant experimental (HPRL1, experimental group, 200 µg/day metoclopramide). Treatments lasted for 50 consecutive days. On day 50, 10 females from each group (control and experimental) were euthanized in the proestrus phase; then, the blood was collected and the lacrimal glands were removed. Thereafter, the remaining females were placed with the mates and continued to receive treatment with saline solution or metoclopramide. On the 6th post-coital day, 10 pregnant females from the control group (CTR2) and 10 pregnant females from the experimental group (HPRL2) were euthanized, after which blood was collected and the lacrimal glands removed. The lacrimal glands were processed for morphological analyses and collagen quantification, and prolactin and sex steroid levels were measured in the blood samples. Data were statistically analyzed using an unpaired Student t test (pHospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2015-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/10393910.6061/clinics/2015(09)07Clinics; Vol. 70 No. 9 (2015); 632-637Clinics; v. 70 n. 9 (2015); 632-637Clinics; Vol. 70 Núm. 9 (2015); 632-6371980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/103939/102481Copyright (c) 2015 Clinicsinfo:eu-repo/semantics/openAccessAraujo, Ariadne Stavare LealSimões, Manuel de JesusVerna, CarinaSimões, Ricardo SantosSoares Júnior, José MariaBaracat, Edmund ChadaGomes, Regina Célia Teixeira2015-09-15T20:12:20Zoai:revistas.usp.br:article/103939Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2015-09-15T20:12:20Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice |
title |
Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice |
spellingShingle |
Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice Araujo, Ariadne Stavare Leal |
title_short |
Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice |
title_full |
Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice |
title_fullStr |
Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice |
title_full_unstemmed |
Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice |
title_sort |
Influence of hyperprolactinemia on collagen fibers in the lacrimal gland of female mice |
author |
Araujo, Ariadne Stavare Leal |
author_facet |
Araujo, Ariadne Stavare Leal Simões, Manuel de Jesus Verna, Carina Simões, Ricardo Santos Soares Júnior, José Maria Baracat, Edmund Chada Gomes, Regina Célia Teixeira |
author_role |
author |
author2 |
Simões, Manuel de Jesus Verna, Carina Simões, Ricardo Santos Soares Júnior, José Maria Baracat, Edmund Chada Gomes, Regina Célia Teixeira |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Araujo, Ariadne Stavare Leal Simões, Manuel de Jesus Verna, Carina Simões, Ricardo Santos Soares Júnior, José Maria Baracat, Edmund Chada Gomes, Regina Célia Teixeira |
description |
OBJECTIVE:To quantify the collagen fibers in the lacrimal gland of female mice with hyperprolactinemia.METHODS:Forty adult female mice were randomly divided into two groups with 20 animals each: nonpregnant control (CTR1, control group, 0.2 mL of saline solution) and nonpregnant experimental (HPRL1, experimental group, 200 µg/day metoclopramide). Treatments lasted for 50 consecutive days. On day 50, 10 females from each group (control and experimental) were euthanized in the proestrus phase; then, the blood was collected and the lacrimal glands were removed. Thereafter, the remaining females were placed with the mates and continued to receive treatment with saline solution or metoclopramide. On the 6th post-coital day, 10 pregnant females from the control group (CTR2) and 10 pregnant females from the experimental group (HPRL2) were euthanized, after which blood was collected and the lacrimal glands removed. The lacrimal glands were processed for morphological analyses and collagen quantification, and prolactin and sex steroid levels were measured in the blood samples. Data were statistically analyzed using an unpaired Student t test (p |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/103939 10.6061/clinics/2015(09)07 |
url |
https://www.revistas.usp.br/clinics/article/view/103939 |
identifier_str_mv |
10.6061/clinics/2015(09)07 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/103939/102481 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2015 Clinics info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2015 Clinics |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 70 No. 9 (2015); 632-637 Clinics; v. 70 n. 9 (2015); 632-637 Clinics; Vol. 70 Núm. 9 (2015); 632-637 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222762220912640 |