Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation

Detalhes bibliográficos
Autor(a) principal: Campos, Jacira Ribeiro
Data de Publicação: 2011
Outros Autores: Rosa-e-Silva, Julio Cesar, Carvalho, Bruno Ramalho, Vireque, Alessandra Aparecida, Silva-de-Sá, Marcos Felipe, Rosa-e-Silva, Ana Carolina Japur de Sá
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/19334
Resumo: OBJECTIVE: To determine the effect of storage duration on cryopreserved ovarian tissue using fresh and frozenthawed samples. METHODS: Seventeen fertile patients underwent an ovarian biopsy during elective laparoscopic tubal ligation. The tissue sample was divided into three parts: one part was processed fresh (FG), and two were slowly frozen, cryopreserved for 30 (G30) or 180 days (G180), thawed and analyzed. Follicular density, follicular viability, and steroidogenic capacity were assessed. RESULTS: We observed no differences between the groups in follicular density, which was assessed in hematoxylin and eosin-stained tissue sections. A heterogeneous follicular distribution was observed in the parenchyma, with a mean density of 361.3±255.4, 454.9±676.3, and 296.8±269.0 follicles/mm3 for FG, G30 and G180, respectively (p = 0.46). Follicular viability was greater in FG (93.4%) when compared with the cryopreserved tissues (70.8% for G30 (p
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spelling Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation Ovarian tissueCryopreservationTissue damageOvarian steroidogenesisTissue culture OBJECTIVE: To determine the effect of storage duration on cryopreserved ovarian tissue using fresh and frozenthawed samples. METHODS: Seventeen fertile patients underwent an ovarian biopsy during elective laparoscopic tubal ligation. The tissue sample was divided into three parts: one part was processed fresh (FG), and two were slowly frozen, cryopreserved for 30 (G30) or 180 days (G180), thawed and analyzed. Follicular density, follicular viability, and steroidogenic capacity were assessed. RESULTS: We observed no differences between the groups in follicular density, which was assessed in hematoxylin and eosin-stained tissue sections. A heterogeneous follicular distribution was observed in the parenchyma, with a mean density of 361.3±255.4, 454.9±676.3, and 296.8±269.0 follicles/mm3 for FG, G30 and G180, respectively (p = 0.46). Follicular viability was greater in FG (93.4%) when compared with the cryopreserved tissues (70.8% for G30 (pHospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1933410.1590/S1807-59322011001200015Clinics; Vol. 66 No. 12 (2011); 2093-2097 Clinics; v. 66 n. 12 (2011); 2093-2097 Clinics; Vol. 66 Núm. 12 (2011); 2093-2097 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/19334/21397Campos, Jacira RibeiroRosa-e-Silva, Julio CesarCarvalho, Bruno RamalhoVireque, Alessandra AparecidaSilva-de-Sá, Marcos FelipeRosa-e-Silva, Ana Carolina Japur de Sáinfo:eu-repo/semantics/openAccess2012-05-23T16:34:41Zoai:revistas.usp.br:article/19334Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T16:34:41Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation
title Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation
spellingShingle Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation
Campos, Jacira Ribeiro
Ovarian tissue
Cryopreservation
Tissue damage
Ovarian steroidogenesis
Tissue culture
title_short Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation
title_full Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation
title_fullStr Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation
title_full_unstemmed Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation
title_sort Cryopreservation time does not decrease follicular viability in ovarian tissue frozen for fertility preservation
author Campos, Jacira Ribeiro
author_facet Campos, Jacira Ribeiro
Rosa-e-Silva, Julio Cesar
Carvalho, Bruno Ramalho
Vireque, Alessandra Aparecida
Silva-de-Sá, Marcos Felipe
Rosa-e-Silva, Ana Carolina Japur de Sá
author_role author
author2 Rosa-e-Silva, Julio Cesar
Carvalho, Bruno Ramalho
Vireque, Alessandra Aparecida
Silva-de-Sá, Marcos Felipe
Rosa-e-Silva, Ana Carolina Japur de Sá
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Campos, Jacira Ribeiro
Rosa-e-Silva, Julio Cesar
Carvalho, Bruno Ramalho
Vireque, Alessandra Aparecida
Silva-de-Sá, Marcos Felipe
Rosa-e-Silva, Ana Carolina Japur de Sá
dc.subject.por.fl_str_mv Ovarian tissue
Cryopreservation
Tissue damage
Ovarian steroidogenesis
Tissue culture
topic Ovarian tissue
Cryopreservation
Tissue damage
Ovarian steroidogenesis
Tissue culture
description OBJECTIVE: To determine the effect of storage duration on cryopreserved ovarian tissue using fresh and frozenthawed samples. METHODS: Seventeen fertile patients underwent an ovarian biopsy during elective laparoscopic tubal ligation. The tissue sample was divided into three parts: one part was processed fresh (FG), and two were slowly frozen, cryopreserved for 30 (G30) or 180 days (G180), thawed and analyzed. Follicular density, follicular viability, and steroidogenic capacity were assessed. RESULTS: We observed no differences between the groups in follicular density, which was assessed in hematoxylin and eosin-stained tissue sections. A heterogeneous follicular distribution was observed in the parenchyma, with a mean density of 361.3±255.4, 454.9±676.3, and 296.8±269.0 follicles/mm3 for FG, G30 and G180, respectively (p = 0.46). Follicular viability was greater in FG (93.4%) when compared with the cryopreserved tissues (70.8% for G30 (p
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/19334
10.1590/S1807-59322011001200015
url https://www.revistas.usp.br/clinics/article/view/19334
identifier_str_mv 10.1590/S1807-59322011001200015
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/19334/21397
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 66 No. 12 (2011); 2093-2097
Clinics; v. 66 n. 12 (2011); 2093-2097
Clinics; Vol. 66 Núm. 12 (2011); 2093-2097
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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