Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains

Detalhes bibliográficos
Autor(a) principal: Nakano, Viviane
Data de Publicação: 2011
Outros Autores: Silva, Amanda do Nascimento e, Merino, Victor Rafael Castillo, Wexler, Hannah M., Avila-Campos, Mario Julio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/19582
Resumo: OBJECTIVE: This study examined the antimicrobial resistance profile and the prevalence of resistance genes in Bacteroides spp. and Parabacteroides distasonis strains isolated from children's intestinal microbiota. METHODS: The susceptibility of these bacteria to 10 antimicrobials was determined using an agar dilution method. β-lactamase activity was assessed by hydrolysis of the chromogenic cephalosporin of 114 Bacteriodales strains isolated from the fecal samples of 39 children, and the presence of resistance genes was tested using a PCR assay. RESULTS: All strains were susceptible to imipenem and metronidazole. The following resistance rates were observed: amoxicillin (93%), amoxicillin/clavulanic acid (47.3%), ampicillin (96.4%), cephalexin (99%), cefoxitin (23%), penicillin (99%), clindamycin (34.2%) and tetracycline (53.5%). P-lactamase production was verified in 92% of the evaluated strains. The presence of the cfiA, cepA, ermF, tetQ and nim genes was observed in 62.3%, 76.3%, 27%, 79.8% and 7.8% of the strains, respectively. CONCLUSIONS: Our results indicate an increase in the resistance to several antibiotics in intestinal Bacteroides spp. and Parabacteroides distasonis and demonstrate that these microorganisms harbor antimicrobial resistance genes that may be transferred to other susceptible intestinal strains.
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spelling Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains Bacteroides spp.Parabacteroides distasonisP-lactamase activityAntimicrobial resistanceResistance genes OBJECTIVE: This study examined the antimicrobial resistance profile and the prevalence of resistance genes in Bacteroides spp. and Parabacteroides distasonis strains isolated from children's intestinal microbiota. METHODS: The susceptibility of these bacteria to 10 antimicrobials was determined using an agar dilution method. β-lactamase activity was assessed by hydrolysis of the chromogenic cephalosporin of 114 Bacteriodales strains isolated from the fecal samples of 39 children, and the presence of resistance genes was tested using a PCR assay. RESULTS: All strains were susceptible to imipenem and metronidazole. The following resistance rates were observed: amoxicillin (93%), amoxicillin/clavulanic acid (47.3%), ampicillin (96.4%), cephalexin (99%), cefoxitin (23%), penicillin (99%), clindamycin (34.2%) and tetracycline (53.5%). P-lactamase production was verified in 92% of the evaluated strains. The presence of the cfiA, cepA, ermF, tetQ and nim genes was observed in 62.3%, 76.3%, 27%, 79.8% and 7.8% of the strains, respectively. CONCLUSIONS: Our results indicate an increase in the resistance to several antibiotics in intestinal Bacteroides spp. and Parabacteroides distasonis and demonstrate that these microorganisms harbor antimicrobial resistance genes that may be transferred to other susceptible intestinal strains. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1958210.1590/S1807-59322011000400004Clinics; Vol. 66 No. 4 (2011); 543-547 Clinics; v. 66 n. 4 (2011); 543-547 Clinics; Vol. 66 Núm. 4 (2011); 543-547 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/19582/21645Nakano, VivianeSilva, Amanda do Nascimento eMerino, Victor Rafael CastilloWexler, Hannah M.Avila-Campos, Mario Julioinfo:eu-repo/semantics/openAccess2012-05-23T16:49:51Zoai:revistas.usp.br:article/19582Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T16:49:51Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains
title Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains
spellingShingle Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains
Nakano, Viviane
Bacteroides spp.
Parabacteroides distasonis
P-lactamase activity
Antimicrobial resistance
Resistance genes
title_short Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains
title_full Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains
title_fullStr Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains
title_full_unstemmed Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains
title_sort Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains
author Nakano, Viviane
author_facet Nakano, Viviane
Silva, Amanda do Nascimento e
Merino, Victor Rafael Castillo
Wexler, Hannah M.
Avila-Campos, Mario Julio
author_role author
author2 Silva, Amanda do Nascimento e
Merino, Victor Rafael Castillo
Wexler, Hannah M.
Avila-Campos, Mario Julio
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Nakano, Viviane
Silva, Amanda do Nascimento e
Merino, Victor Rafael Castillo
Wexler, Hannah M.
Avila-Campos, Mario Julio
dc.subject.por.fl_str_mv Bacteroides spp.
Parabacteroides distasonis
P-lactamase activity
Antimicrobial resistance
Resistance genes
topic Bacteroides spp.
Parabacteroides distasonis
P-lactamase activity
Antimicrobial resistance
Resistance genes
description OBJECTIVE: This study examined the antimicrobial resistance profile and the prevalence of resistance genes in Bacteroides spp. and Parabacteroides distasonis strains isolated from children's intestinal microbiota. METHODS: The susceptibility of these bacteria to 10 antimicrobials was determined using an agar dilution method. β-lactamase activity was assessed by hydrolysis of the chromogenic cephalosporin of 114 Bacteriodales strains isolated from the fecal samples of 39 children, and the presence of resistance genes was tested using a PCR assay. RESULTS: All strains were susceptible to imipenem and metronidazole. The following resistance rates were observed: amoxicillin (93%), amoxicillin/clavulanic acid (47.3%), ampicillin (96.4%), cephalexin (99%), cefoxitin (23%), penicillin (99%), clindamycin (34.2%) and tetracycline (53.5%). P-lactamase production was verified in 92% of the evaluated strains. The presence of the cfiA, cepA, ermF, tetQ and nim genes was observed in 62.3%, 76.3%, 27%, 79.8% and 7.8% of the strains, respectively. CONCLUSIONS: Our results indicate an increase in the resistance to several antibiotics in intestinal Bacteroides spp. and Parabacteroides distasonis and demonstrate that these microorganisms harbor antimicrobial resistance genes that may be transferred to other susceptible intestinal strains.
publishDate 2011
dc.date.none.fl_str_mv 2011-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/19582
10.1590/S1807-59322011000400004
url https://www.revistas.usp.br/clinics/article/view/19582
identifier_str_mv 10.1590/S1807-59322011000400004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/19582/21645
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 66 No. 4 (2011); 543-547
Clinics; v. 66 n. 4 (2011); 543-547
Clinics; Vol. 66 Núm. 4 (2011); 543-547
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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