Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/19582 |
Resumo: | OBJECTIVE: This study examined the antimicrobial resistance profile and the prevalence of resistance genes in Bacteroides spp. and Parabacteroides distasonis strains isolated from children's intestinal microbiota. METHODS: The susceptibility of these bacteria to 10 antimicrobials was determined using an agar dilution method. β-lactamase activity was assessed by hydrolysis of the chromogenic cephalosporin of 114 Bacteriodales strains isolated from the fecal samples of 39 children, and the presence of resistance genes was tested using a PCR assay. RESULTS: All strains were susceptible to imipenem and metronidazole. The following resistance rates were observed: amoxicillin (93%), amoxicillin/clavulanic acid (47.3%), ampicillin (96.4%), cephalexin (99%), cefoxitin (23%), penicillin (99%), clindamycin (34.2%) and tetracycline (53.5%). P-lactamase production was verified in 92% of the evaluated strains. The presence of the cfiA, cepA, ermF, tetQ and nim genes was observed in 62.3%, 76.3%, 27%, 79.8% and 7.8% of the strains, respectively. CONCLUSIONS: Our results indicate an increase in the resistance to several antibiotics in intestinal Bacteroides spp. and Parabacteroides distasonis and demonstrate that these microorganisms harbor antimicrobial resistance genes that may be transferred to other susceptible intestinal strains. |
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Clinics |
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Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains Bacteroides spp.Parabacteroides distasonisP-lactamase activityAntimicrobial resistanceResistance genes OBJECTIVE: This study examined the antimicrobial resistance profile and the prevalence of resistance genes in Bacteroides spp. and Parabacteroides distasonis strains isolated from children's intestinal microbiota. METHODS: The susceptibility of these bacteria to 10 antimicrobials was determined using an agar dilution method. β-lactamase activity was assessed by hydrolysis of the chromogenic cephalosporin of 114 Bacteriodales strains isolated from the fecal samples of 39 children, and the presence of resistance genes was tested using a PCR assay. RESULTS: All strains were susceptible to imipenem and metronidazole. The following resistance rates were observed: amoxicillin (93%), amoxicillin/clavulanic acid (47.3%), ampicillin (96.4%), cephalexin (99%), cefoxitin (23%), penicillin (99%), clindamycin (34.2%) and tetracycline (53.5%). P-lactamase production was verified in 92% of the evaluated strains. The presence of the cfiA, cepA, ermF, tetQ and nim genes was observed in 62.3%, 76.3%, 27%, 79.8% and 7.8% of the strains, respectively. CONCLUSIONS: Our results indicate an increase in the resistance to several antibiotics in intestinal Bacteroides spp. and Parabacteroides distasonis and demonstrate that these microorganisms harbor antimicrobial resistance genes that may be transferred to other susceptible intestinal strains. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2011-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/1958210.1590/S1807-59322011000400004Clinics; Vol. 66 No. 4 (2011); 543-547 Clinics; v. 66 n. 4 (2011); 543-547 Clinics; Vol. 66 Núm. 4 (2011); 543-547 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/19582/21645Nakano, VivianeSilva, Amanda do Nascimento eMerino, Victor Rafael CastilloWexler, Hannah M.Avila-Campos, Mario Julioinfo:eu-repo/semantics/openAccess2012-05-23T16:49:51Zoai:revistas.usp.br:article/19582Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2012-05-23T16:49:51Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains |
title |
Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains |
spellingShingle |
Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains Nakano, Viviane Bacteroides spp. Parabacteroides distasonis P-lactamase activity Antimicrobial resistance Resistance genes |
title_short |
Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains |
title_full |
Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains |
title_fullStr |
Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains |
title_full_unstemmed |
Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains |
title_sort |
Antimicrobial resistance and prevalence of resistance genes in intestinal Bacteroidales strains |
author |
Nakano, Viviane |
author_facet |
Nakano, Viviane Silva, Amanda do Nascimento e Merino, Victor Rafael Castillo Wexler, Hannah M. Avila-Campos, Mario Julio |
author_role |
author |
author2 |
Silva, Amanda do Nascimento e Merino, Victor Rafael Castillo Wexler, Hannah M. Avila-Campos, Mario Julio |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Nakano, Viviane Silva, Amanda do Nascimento e Merino, Victor Rafael Castillo Wexler, Hannah M. Avila-Campos, Mario Julio |
dc.subject.por.fl_str_mv |
Bacteroides spp. Parabacteroides distasonis P-lactamase activity Antimicrobial resistance Resistance genes |
topic |
Bacteroides spp. Parabacteroides distasonis P-lactamase activity Antimicrobial resistance Resistance genes |
description |
OBJECTIVE: This study examined the antimicrobial resistance profile and the prevalence of resistance genes in Bacteroides spp. and Parabacteroides distasonis strains isolated from children's intestinal microbiota. METHODS: The susceptibility of these bacteria to 10 antimicrobials was determined using an agar dilution method. β-lactamase activity was assessed by hydrolysis of the chromogenic cephalosporin of 114 Bacteriodales strains isolated from the fecal samples of 39 children, and the presence of resistance genes was tested using a PCR assay. RESULTS: All strains were susceptible to imipenem and metronidazole. The following resistance rates were observed: amoxicillin (93%), amoxicillin/clavulanic acid (47.3%), ampicillin (96.4%), cephalexin (99%), cefoxitin (23%), penicillin (99%), clindamycin (34.2%) and tetracycline (53.5%). P-lactamase production was verified in 92% of the evaluated strains. The presence of the cfiA, cepA, ermF, tetQ and nim genes was observed in 62.3%, 76.3%, 27%, 79.8% and 7.8% of the strains, respectively. CONCLUSIONS: Our results indicate an increase in the resistance to several antibiotics in intestinal Bacteroides spp. and Parabacteroides distasonis and demonstrate that these microorganisms harbor antimicrobial resistance genes that may be transferred to other susceptible intestinal strains. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19582 10.1590/S1807-59322011000400004 |
url |
https://www.revistas.usp.br/clinics/article/view/19582 |
identifier_str_mv |
10.1590/S1807-59322011000400004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/19582/21645 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 66 No. 4 (2011); 543-547 Clinics; v. 66 n. 4 (2011); 543-547 Clinics; Vol. 66 Núm. 4 (2011); 543-547 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222757813747712 |