Hypertonic saline solution reduces the inflammatory response in endotoxemic rats
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Clinics |
Texto Completo: | https://www.revistas.usp.br/clinics/article/view/52293 |
Resumo: | OBJECTIVE: Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS: We measured cytokines (serum and gut), nitrite, and lipid peroxidation (TBARS) as indicators of oxidative stress in the gut. Rats were divided into four groups: control group (C) that did not receive lipopolysaccharide; lipopolysaccharide injection without treatment (LPS); lipopolysaccharide injection with saline treatment (LPS +S); and lipopolysaccharide injection with hypertonic saline treatment (LPS +H). Serum and intestine were collected. Measurements were taken at 1.5, 8, and 24 h after lipopolysaccharide administration. RESULTS: Of the four groups, the LPS +H group had the highest survival rate. Hypertonic saline solution treatment led to lower levels of IL-6, IL-10, nitric oxide, and thiobarbituric acid reactive substances compared to 0.9% normal saline. In addition, hypertonic saline treatment resulted in a lower mortality compared to 0.9% normal saline treatment in endotoxemic rats. Volume replacement reduced levels of inflammatory mediators in the plasma and gut. CONCLUSION: Hypertonic saline treatment reduced mortality and lowered levels of inflammatory mediators in endotoxemic rats. Hypertonic saline also has the advantage of requiring less volume replacement. |
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Hypertonic saline solution reduces the inflammatory response in endotoxemic rats LPSHypertonic SolutionInterleukinOxidative StressIntestine OBJECTIVE: Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS: We measured cytokines (serum and gut), nitrite, and lipid peroxidation (TBARS) as indicators of oxidative stress in the gut. Rats were divided into four groups: control group (C) that did not receive lipopolysaccharide; lipopolysaccharide injection without treatment (LPS); lipopolysaccharide injection with saline treatment (LPS +S); and lipopolysaccharide injection with hypertonic saline treatment (LPS +H). Serum and intestine were collected. Measurements were taken at 1.5, 8, and 24 h after lipopolysaccharide administration. RESULTS: Of the four groups, the LPS +H group had the highest survival rate. Hypertonic saline solution treatment led to lower levels of IL-6, IL-10, nitric oxide, and thiobarbituric acid reactive substances compared to 0.9% normal saline. In addition, hypertonic saline treatment resulted in a lower mortality compared to 0.9% normal saline treatment in endotoxemic rats. Volume replacement reduced levels of inflammatory mediators in the plasma and gut. CONCLUSION: Hypertonic saline treatment reduced mortality and lowered levels of inflammatory mediators in endotoxemic rats. Hypertonic saline also has the advantage of requiring less volume replacement. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/5229310.6061/clinics/2012(12)18Clinics; Vol. 67 No. 12 (2012); 1463-1468 Clinics; v. 67 n. 12 (2012); 1463-1468 Clinics; Vol. 67 Núm. 12 (2012); 1463-1468 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/52293/56320Theobaldo, Mariana CardilloBarbeiro, Hermes VieiraBarbeiro, Denise FredianiPetroni, RicardoSoriano, Francisco Garciainfo:eu-repo/semantics/openAccess2013-03-09T13:27:19Zoai:revistas.usp.br:article/52293Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2013-03-09T13:27:19Clinics - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Hypertonic saline solution reduces the inflammatory response in endotoxemic rats |
title |
Hypertonic saline solution reduces the inflammatory response in endotoxemic rats |
spellingShingle |
Hypertonic saline solution reduces the inflammatory response in endotoxemic rats Theobaldo, Mariana Cardillo LPS Hypertonic Solution Interleukin Oxidative Stress Intestine |
title_short |
Hypertonic saline solution reduces the inflammatory response in endotoxemic rats |
title_full |
Hypertonic saline solution reduces the inflammatory response in endotoxemic rats |
title_fullStr |
Hypertonic saline solution reduces the inflammatory response in endotoxemic rats |
title_full_unstemmed |
Hypertonic saline solution reduces the inflammatory response in endotoxemic rats |
title_sort |
Hypertonic saline solution reduces the inflammatory response in endotoxemic rats |
author |
Theobaldo, Mariana Cardillo |
author_facet |
Theobaldo, Mariana Cardillo Barbeiro, Hermes Vieira Barbeiro, Denise Frediani Petroni, Ricardo Soriano, Francisco Garcia |
author_role |
author |
author2 |
Barbeiro, Hermes Vieira Barbeiro, Denise Frediani Petroni, Ricardo Soriano, Francisco Garcia |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Theobaldo, Mariana Cardillo Barbeiro, Hermes Vieira Barbeiro, Denise Frediani Petroni, Ricardo Soriano, Francisco Garcia |
dc.subject.por.fl_str_mv |
LPS Hypertonic Solution Interleukin Oxidative Stress Intestine |
topic |
LPS Hypertonic Solution Interleukin Oxidative Stress Intestine |
description |
OBJECTIVE: Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS: We measured cytokines (serum and gut), nitrite, and lipid peroxidation (TBARS) as indicators of oxidative stress in the gut. Rats were divided into four groups: control group (C) that did not receive lipopolysaccharide; lipopolysaccharide injection without treatment (LPS); lipopolysaccharide injection with saline treatment (LPS +S); and lipopolysaccharide injection with hypertonic saline treatment (LPS +H). Serum and intestine were collected. Measurements were taken at 1.5, 8, and 24 h after lipopolysaccharide administration. RESULTS: Of the four groups, the LPS +H group had the highest survival rate. Hypertonic saline solution treatment led to lower levels of IL-6, IL-10, nitric oxide, and thiobarbituric acid reactive substances compared to 0.9% normal saline. In addition, hypertonic saline treatment resulted in a lower mortality compared to 0.9% normal saline treatment in endotoxemic rats. Volume replacement reduced levels of inflammatory mediators in the plasma and gut. CONCLUSION: Hypertonic saline treatment reduced mortality and lowered levels of inflammatory mediators in endotoxemic rats. Hypertonic saline also has the advantage of requiring less volume replacement. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/52293 10.6061/clinics/2012(12)18 |
url |
https://www.revistas.usp.br/clinics/article/view/52293 |
identifier_str_mv |
10.6061/clinics/2012(12)18 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/clinics/article/view/52293/56320 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
publisher.none.fl_str_mv |
Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo |
dc.source.none.fl_str_mv |
Clinics; Vol. 67 No. 12 (2012); 1463-1468 Clinics; v. 67 n. 12 (2012); 1463-1468 Clinics; Vol. 67 Núm. 12 (2012); 1463-1468 1980-5322 1807-5932 reponame:Clinics instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Clinics |
collection |
Clinics |
repository.name.fl_str_mv |
Clinics - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||clinics@hc.fm.usp.br |
_version_ |
1800222759347814400 |