Hypertonic saline solution reduces the inflammatory response in endotoxemic rats

Detalhes bibliográficos
Autor(a) principal: Theobaldo, Mariana Cardillo
Data de Publicação: 2012
Outros Autores: Barbeiro, Hermes Vieira, Barbeiro, Denise Frediani, Petroni, Ricardo, Soriano, Francisco Garcia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Clinics
Texto Completo: https://www.revistas.usp.br/clinics/article/view/52293
Resumo: OBJECTIVE: Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS: We measured cytokines (serum and gut), nitrite, and lipid peroxidation (TBARS) as indicators of oxidative stress in the gut. Rats were divided into four groups: control group (C) that did not receive lipopolysaccharide; lipopolysaccharide injection without treatment (LPS); lipopolysaccharide injection with saline treatment (LPS +S); and lipopolysaccharide injection with hypertonic saline treatment (LPS +H). Serum and intestine were collected. Measurements were taken at 1.5, 8, and 24 h after lipopolysaccharide administration. RESULTS: Of the four groups, the LPS +H group had the highest survival rate. Hypertonic saline solution treatment led to lower levels of IL-6, IL-10, nitric oxide, and thiobarbituric acid reactive substances compared to 0.9% normal saline. In addition, hypertonic saline treatment resulted in a lower mortality compared to 0.9% normal saline treatment in endotoxemic rats. Volume replacement reduced levels of inflammatory mediators in the plasma and gut. CONCLUSION: Hypertonic saline treatment reduced mortality and lowered levels of inflammatory mediators in endotoxemic rats. Hypertonic saline also has the advantage of requiring less volume replacement.
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spelling Hypertonic saline solution reduces the inflammatory response in endotoxemic rats LPSHypertonic SolutionInterleukinOxidative StressIntestine OBJECTIVE: Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS: We measured cytokines (serum and gut), nitrite, and lipid peroxidation (TBARS) as indicators of oxidative stress in the gut. Rats were divided into four groups: control group (C) that did not receive lipopolysaccharide; lipopolysaccharide injection without treatment (LPS); lipopolysaccharide injection with saline treatment (LPS +S); and lipopolysaccharide injection with hypertonic saline treatment (LPS +H). Serum and intestine were collected. Measurements were taken at 1.5, 8, and 24 h after lipopolysaccharide administration. RESULTS: Of the four groups, the LPS +H group had the highest survival rate. Hypertonic saline solution treatment led to lower levels of IL-6, IL-10, nitric oxide, and thiobarbituric acid reactive substances compared to 0.9% normal saline. In addition, hypertonic saline treatment resulted in a lower mortality compared to 0.9% normal saline treatment in endotoxemic rats. Volume replacement reduced levels of inflammatory mediators in the plasma and gut. CONCLUSION: Hypertonic saline treatment reduced mortality and lowered levels of inflammatory mediators in endotoxemic rats. Hypertonic saline also has the advantage of requiring less volume replacement. Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/clinics/article/view/5229310.6061/clinics/2012(12)18Clinics; Vol. 67 No. 12 (2012); 1463-1468 Clinics; v. 67 n. 12 (2012); 1463-1468 Clinics; Vol. 67 Núm. 12 (2012); 1463-1468 1980-53221807-5932reponame:Clinicsinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/clinics/article/view/52293/56320Theobaldo, Mariana CardilloBarbeiro, Hermes VieiraBarbeiro, Denise FredianiPetroni, RicardoSoriano, Francisco Garciainfo:eu-repo/semantics/openAccess2013-03-09T13:27:19Zoai:revistas.usp.br:article/52293Revistahttps://www.revistas.usp.br/clinicsPUBhttps://www.revistas.usp.br/clinics/oai||clinics@hc.fm.usp.br1980-53221807-5932opendoar:2013-03-09T13:27:19Clinics - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Hypertonic saline solution reduces the inflammatory response in endotoxemic rats
title Hypertonic saline solution reduces the inflammatory response in endotoxemic rats
spellingShingle Hypertonic saline solution reduces the inflammatory response in endotoxemic rats
Theobaldo, Mariana Cardillo
LPS
Hypertonic Solution
Interleukin
Oxidative Stress
Intestine
title_short Hypertonic saline solution reduces the inflammatory response in endotoxemic rats
title_full Hypertonic saline solution reduces the inflammatory response in endotoxemic rats
title_fullStr Hypertonic saline solution reduces the inflammatory response in endotoxemic rats
title_full_unstemmed Hypertonic saline solution reduces the inflammatory response in endotoxemic rats
title_sort Hypertonic saline solution reduces the inflammatory response in endotoxemic rats
author Theobaldo, Mariana Cardillo
author_facet Theobaldo, Mariana Cardillo
Barbeiro, Hermes Vieira
Barbeiro, Denise Frediani
Petroni, Ricardo
Soriano, Francisco Garcia
author_role author
author2 Barbeiro, Hermes Vieira
Barbeiro, Denise Frediani
Petroni, Ricardo
Soriano, Francisco Garcia
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Theobaldo, Mariana Cardillo
Barbeiro, Hermes Vieira
Barbeiro, Denise Frediani
Petroni, Ricardo
Soriano, Francisco Garcia
dc.subject.por.fl_str_mv LPS
Hypertonic Solution
Interleukin
Oxidative Stress
Intestine
topic LPS
Hypertonic Solution
Interleukin
Oxidative Stress
Intestine
description OBJECTIVE: Volume replacement in septic patients improves hemodynamic stability. This effect can reduce the inflammatory response. The objective of this study was to evaluate the effect of 7.5% hypertonic saline solution versus 0.9% normal saline solution for volume replacement during an inflammatory response in endotoxemic rats. METHODS: We measured cytokines (serum and gut), nitrite, and lipid peroxidation (TBARS) as indicators of oxidative stress in the gut. Rats were divided into four groups: control group (C) that did not receive lipopolysaccharide; lipopolysaccharide injection without treatment (LPS); lipopolysaccharide injection with saline treatment (LPS +S); and lipopolysaccharide injection with hypertonic saline treatment (LPS +H). Serum and intestine were collected. Measurements were taken at 1.5, 8, and 24 h after lipopolysaccharide administration. RESULTS: Of the four groups, the LPS +H group had the highest survival rate. Hypertonic saline solution treatment led to lower levels of IL-6, IL-10, nitric oxide, and thiobarbituric acid reactive substances compared to 0.9% normal saline. In addition, hypertonic saline treatment resulted in a lower mortality compared to 0.9% normal saline treatment in endotoxemic rats. Volume replacement reduced levels of inflammatory mediators in the plasma and gut. CONCLUSION: Hypertonic saline treatment reduced mortality and lowered levels of inflammatory mediators in endotoxemic rats. Hypertonic saline also has the advantage of requiring less volume replacement.
publishDate 2012
dc.date.none.fl_str_mv 2012-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/clinics/article/view/52293
10.6061/clinics/2012(12)18
url https://www.revistas.usp.br/clinics/article/view/52293
identifier_str_mv 10.6061/clinics/2012(12)18
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/clinics/article/view/52293/56320
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
publisher.none.fl_str_mv Hospital das Clínicas, Faculdade de Medicina, Universidade de São Paulo
dc.source.none.fl_str_mv Clinics; Vol. 67 No. 12 (2012); 1463-1468
Clinics; v. 67 n. 12 (2012); 1463-1468
Clinics; Vol. 67 Núm. 12 (2012); 1463-1468
1980-5322
1807-5932
reponame:Clinics
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Clinics
collection Clinics
repository.name.fl_str_mv Clinics - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||clinics@hc.fm.usp.br
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