Detection of anti-Giardia lamblia serum antibody among children of day care centers

• Autor(a) principal: Guimarães, Semíramis
• Data de Publicação: 2002
• Outros Autores: Sogayar, Maria Inês L
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Revista de Saúde Pública
• Texto Completo: https://www.revistas.usp.br/rsp/article/view/25303
• topic: Giardia lamblia^i2^simunolo; Anticorpos antiprotozoários; Testes sorológicos; Elisa; Técnica de imunofluorescência indireta; Fezes^i2^sparasitolo; Creches; Giardia lamblia^i1^simmunol; Antibodies^i1^sprotoz; Serologic tests; Enzyme-linked immunosorbent assay; Fluorescent antibody technique; Feces^i1^sparasitol; Child day care centers

OBJETIVES: To detect anti-Giardia lamblia serum antibodies in healthy children attending public day care centers and to assess serological tests as tools for estimating the prevalence of G. lamblia in endemic areas. METHODS: Three separate stool specimens and filter paper blood samples were collected from 147 children ranging from 0 to 6 years old. Each stool sample was processed using spontaneous sedimentation and zinc sulfate flotation methods. Blood samples were tested by indirect immunofluorescence (IIF) and enzyme-linked immunosorbent assay (ELISA) for Giardia IgG. RESULTS AND CONCLUSIONS: Of 147 individuals tested, 93 (63.3%) showed Giardia cysts in their feces. Using IIF and ELISA, serum antibodies were detected in 93 (63.3%) and 100 (68%) samples , respectively. Sensitivity of IIF and ELISA was 82% and 72%, respectively. However, ELISA revealed to be less specific (39%) than IIF (70%). IIF also showed a higher concordance with microscopic examination than ELISA.