Identification of the fungic microbiota isolated in areas considered aseptic

Detalhes bibliográficos
Autor(a) principal: Almeida, Maria Elisabete Sbrogio de
Data de Publicação: 1988
Outros Autores: Martini, Maria Helena, Porto, Edward, Camargo, Ana Maria Martins de, Rizzo, Edda de, Lacaz, Carlos da Silva
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Saúde Pública
Texto Completo: https://www.revistas.usp.br/rsp/article/view/23495
Resumo: The isolation and identification of the fungic microbiota present in areas considered aseptic (open and/or restricted, with or without air filtration (HEPA filters) where production of immunobiologicals is carried out)were investigated Three exposures were made with appropriate culture media, in different seasons of the year, before and immediately after the performance of the technical work. Agar-Sabouraud and agar-soy media supplemented with 0.2% yeast extract and without chloranphenicol had their efficacy tests in isolating the filamentous fungia e (molds) and yeasts in the light of the number of cultures grown and the frequency exhibited by the various specimens isolated. Sixty-seven specimens (64 of filamentous fungiae (molds) and three yeasts) have been identified. Of the filamentous fungiae (molds), 54 belonged to 22 genera of the division Deuteromycota, families Moniliaceae and Dematiaceae, five were included in the order Agonomycetales (Mycelia Sterilia), and one was classified in the order Sphaeropsidales, class Coelomycetes. Of the division Zygomycota, order Mucorales, family Mucoraceae, only one specimen could be identified as to genus. The three yeasts, belonging also to the division Deuteromycota (Fungi Imperfecti), family Cryptococcaceae, were identified as two Rhodotorula rubra and one Torulopsis candida. It was demonstrated that the number of colonies isolated in the areas studied increased after the performance of technical maneuvers and that air filtration by means of HEPA filters, reducing to number of colonies isolated in restricted areas, increased safety and is consequently recommended for working areas where successful or unsuccessful results depend on a low incidence of contamination.
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spelling Identification of the fungic microbiota isolated in areas considered aseptic Identificação da microbiota fúngica de ambientes considerados assépticos Fungos^i1^sisolameLeveduras^i1^sisolameAmbiente construídoAssepsiaMicrobiologia ambientalFungs^i2^sisolatYeasts^i2^sisolatEnvironmentcontrolledAsepsisEnvironmental microbiology The isolation and identification of the fungic microbiota present in areas considered aseptic (open and/or restricted, with or without air filtration (HEPA filters) where production of immunobiologicals is carried out)were investigated Three exposures were made with appropriate culture media, in different seasons of the year, before and immediately after the performance of the technical work. Agar-Sabouraud and agar-soy media supplemented with 0.2% yeast extract and without chloranphenicol had their efficacy tests in isolating the filamentous fungia e (molds) and yeasts in the light of the number of cultures grown and the frequency exhibited by the various specimens isolated. Sixty-seven specimens (64 of filamentous fungiae (molds) and three yeasts) have been identified. Of the filamentous fungiae (molds), 54 belonged to 22 genera of the division Deuteromycota, families Moniliaceae and Dematiaceae, five were included in the order Agonomycetales (Mycelia Sterilia), and one was classified in the order Sphaeropsidales, class Coelomycetes. Of the division Zygomycota, order Mucorales, family Mucoraceae, only one specimen could be identified as to genus. The three yeasts, belonging also to the division Deuteromycota (Fungi Imperfecti), family Cryptococcaceae, were identified as two Rhodotorula rubra and one Torulopsis candida. It was demonstrated that the number of colonies isolated in the areas studied increased after the performance of technical maneuvers and that air filtration by means of HEPA filters, reducing to number of colonies isolated in restricted areas, increased safety and is consequently recommended for working areas where successful or unsuccessful results depend on a low incidence of contamination. Objetivou-se isolar e identificar a microbiota fúngica em ambientes considerados assépticos, através de exposições com meios de cultivo adequados, em três épocas distintas do ano, antes e imediatamente após as manobras técnicas realizadas em três áreas de trabalho: ambiente aberto, ambiente fechado sem filtração de ar e ambiente fechado com filtração de ar, utilizadas em produção de imunobiológicos. Os meios ágar-Sabouraud e ágar-soja, enriquecidos com 0,2% de extrato de levedura e sem cloranfenicol, foram estudados quanto à sua eficácia no isolamento de bolores e leveduras, considerando-se o número de colônias desenvolvidas e a freqüência dos diversos fungos isolados. Isolaram-se 67 espécimens, sendo 64 fungos filamentosos (bolores) e três leveduras. Dos bolores, 54 pertenciam a 22 gêneros da divisão Deuteromycota, famílias Moniliaceae e Dematiaceae, cinco amostras filamentosas foram incluídas na ordem Agonomycetales (Mycelia Sterilia), e uma amostra foi classificada na divisão Deuteromycota, ordem Sphaeropsidales, classe Coelomycetes. Da divisão Zygomycota, ordem Mucorales, família Mucoraceae, um único mucoráceo foi identificado até gênero. As três leveduras pertenciam também à divisão Deuteromycota (Fungi Imperfecti), família Cryptococcaceae, e foram identificadas como sendo duas Rhodotorula rubra e uma Torulopsis candida. Comprovou-se que o número de colônias isoladas aumentou após a realização das monobras técnicas e que a filtração de ar através de filtros tipo HEPA, reduzindo o número de colônias isoladas nos ambientes fechados, aumenta a segurança do trabalho; comumente é recomendada para áreas de atividade técnica cujos resultados satisfatórios estão diretamente relacionados com uma baixa incidência de contaminantes. Universidade de São Paulo. Faculdade de Saúde Pública1988-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/2349510.1590/S0034-89101988000300006Revista de Saúde Pública; Vol. 22 No. 3 (1988); 201-206 Revista de Saúde Pública; Vol. 22 Núm. 3 (1988); 201-206 Revista de Saúde Pública; v. 22 n. 3 (1988); 201-206 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/23495/25532Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessAlmeida, Maria Elisabete Sbrogio deMartini, Maria HelenaPorto, EdwardCamargo, Ana Maria Martins deRizzo, Edda deLacaz, Carlos da Silva2012-05-28T16:54:38Zoai:revistas.usp.br:article/23495Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-05-28T16:54:38Revista de Saúde Pública - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Identification of the fungic microbiota isolated in areas considered aseptic
Identificação da microbiota fúngica de ambientes considerados assépticos
title Identification of the fungic microbiota isolated in areas considered aseptic
spellingShingle Identification of the fungic microbiota isolated in areas considered aseptic
Almeida, Maria Elisabete Sbrogio de
Fungos^i1^sisolame
Leveduras^i1^sisolame
Ambiente construído
Assepsia
Microbiologia ambiental
Fungs^i2^sisolat
Yeasts^i2^sisolat
Environment
controlled
Asepsis
Environmental microbiology
title_short Identification of the fungic microbiota isolated in areas considered aseptic
title_full Identification of the fungic microbiota isolated in areas considered aseptic
title_fullStr Identification of the fungic microbiota isolated in areas considered aseptic
title_full_unstemmed Identification of the fungic microbiota isolated in areas considered aseptic
title_sort Identification of the fungic microbiota isolated in areas considered aseptic
author Almeida, Maria Elisabete Sbrogio de
author_facet Almeida, Maria Elisabete Sbrogio de
Martini, Maria Helena
Porto, Edward
Camargo, Ana Maria Martins de
Rizzo, Edda de
Lacaz, Carlos da Silva
author_role author
author2 Martini, Maria Helena
Porto, Edward
Camargo, Ana Maria Martins de
Rizzo, Edda de
Lacaz, Carlos da Silva
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Almeida, Maria Elisabete Sbrogio de
Martini, Maria Helena
Porto, Edward
Camargo, Ana Maria Martins de
Rizzo, Edda de
Lacaz, Carlos da Silva
dc.subject.por.fl_str_mv Fungos^i1^sisolame
Leveduras^i1^sisolame
Ambiente construído
Assepsia
Microbiologia ambiental
Fungs^i2^sisolat
Yeasts^i2^sisolat
Environment
controlled
Asepsis
Environmental microbiology
topic Fungos^i1^sisolame
Leveduras^i1^sisolame
Ambiente construído
Assepsia
Microbiologia ambiental
Fungs^i2^sisolat
Yeasts^i2^sisolat
Environment
controlled
Asepsis
Environmental microbiology
description The isolation and identification of the fungic microbiota present in areas considered aseptic (open and/or restricted, with or without air filtration (HEPA filters) where production of immunobiologicals is carried out)were investigated Three exposures were made with appropriate culture media, in different seasons of the year, before and immediately after the performance of the technical work. Agar-Sabouraud and agar-soy media supplemented with 0.2% yeast extract and without chloranphenicol had their efficacy tests in isolating the filamentous fungia e (molds) and yeasts in the light of the number of cultures grown and the frequency exhibited by the various specimens isolated. Sixty-seven specimens (64 of filamentous fungiae (molds) and three yeasts) have been identified. Of the filamentous fungiae (molds), 54 belonged to 22 genera of the division Deuteromycota, families Moniliaceae and Dematiaceae, five were included in the order Agonomycetales (Mycelia Sterilia), and one was classified in the order Sphaeropsidales, class Coelomycetes. Of the division Zygomycota, order Mucorales, family Mucoraceae, only one specimen could be identified as to genus. The three yeasts, belonging also to the division Deuteromycota (Fungi Imperfecti), family Cryptococcaceae, were identified as two Rhodotorula rubra and one Torulopsis candida. It was demonstrated that the number of colonies isolated in the areas studied increased after the performance of technical maneuvers and that air filtration by means of HEPA filters, reducing to number of colonies isolated in restricted areas, increased safety and is consequently recommended for working areas where successful or unsuccessful results depend on a low incidence of contamination.
publishDate 1988
dc.date.none.fl_str_mv 1988-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rsp/article/view/23495
10.1590/S0034-89101988000300006
url https://www.revistas.usp.br/rsp/article/view/23495
identifier_str_mv 10.1590/S0034-89101988000300006
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rsp/article/view/23495/25532
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista de Saúde Pública
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista de Saúde Pública
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
dc.source.none.fl_str_mv Revista de Saúde Pública; Vol. 22 No. 3 (1988); 201-206
Revista de Saúde Pública; Vol. 22 Núm. 3 (1988); 201-206
Revista de Saúde Pública; v. 22 n. 3 (1988); 201-206
1518-8787
0034-8910
reponame:Revista de Saúde Pública
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista de Saúde Pública
collection Revista de Saúde Pública
repository.name.fl_str_mv Revista de Saúde Pública - Universidade de São Paulo (USP)
repository.mail.fl_str_mv revsp@org.usp.br||revsp1@usp.br
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