Identification of the fungic microbiota isolated in areas considered aseptic
Autor(a) principal: | |
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Data de Publicação: | 1988 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Saúde Pública |
Texto Completo: | https://www.revistas.usp.br/rsp/article/view/23495 |
Resumo: | The isolation and identification of the fungic microbiota present in areas considered aseptic (open and/or restricted, with or without air filtration (HEPA filters) where production of immunobiologicals is carried out)were investigated Three exposures were made with appropriate culture media, in different seasons of the year, before and immediately after the performance of the technical work. Agar-Sabouraud and agar-soy media supplemented with 0.2% yeast extract and without chloranphenicol had their efficacy tests in isolating the filamentous fungia e (molds) and yeasts in the light of the number of cultures grown and the frequency exhibited by the various specimens isolated. Sixty-seven specimens (64 of filamentous fungiae (molds) and three yeasts) have been identified. Of the filamentous fungiae (molds), 54 belonged to 22 genera of the division Deuteromycota, families Moniliaceae and Dematiaceae, five were included in the order Agonomycetales (Mycelia Sterilia), and one was classified in the order Sphaeropsidales, class Coelomycetes. Of the division Zygomycota, order Mucorales, family Mucoraceae, only one specimen could be identified as to genus. The three yeasts, belonging also to the division Deuteromycota (Fungi Imperfecti), family Cryptococcaceae, were identified as two Rhodotorula rubra and one Torulopsis candida. It was demonstrated that the number of colonies isolated in the areas studied increased after the performance of technical maneuvers and that air filtration by means of HEPA filters, reducing to number of colonies isolated in restricted areas, increased safety and is consequently recommended for working areas where successful or unsuccessful results depend on a low incidence of contamination. |
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Identification of the fungic microbiota isolated in areas considered aseptic Identificação da microbiota fúngica de ambientes considerados assépticos Fungos^i1^sisolameLeveduras^i1^sisolameAmbiente construídoAssepsiaMicrobiologia ambientalFungs^i2^sisolatYeasts^i2^sisolatEnvironmentcontrolledAsepsisEnvironmental microbiology The isolation and identification of the fungic microbiota present in areas considered aseptic (open and/or restricted, with or without air filtration (HEPA filters) where production of immunobiologicals is carried out)were investigated Three exposures were made with appropriate culture media, in different seasons of the year, before and immediately after the performance of the technical work. Agar-Sabouraud and agar-soy media supplemented with 0.2% yeast extract and without chloranphenicol had their efficacy tests in isolating the filamentous fungia e (molds) and yeasts in the light of the number of cultures grown and the frequency exhibited by the various specimens isolated. Sixty-seven specimens (64 of filamentous fungiae (molds) and three yeasts) have been identified. Of the filamentous fungiae (molds), 54 belonged to 22 genera of the division Deuteromycota, families Moniliaceae and Dematiaceae, five were included in the order Agonomycetales (Mycelia Sterilia), and one was classified in the order Sphaeropsidales, class Coelomycetes. Of the division Zygomycota, order Mucorales, family Mucoraceae, only one specimen could be identified as to genus. The three yeasts, belonging also to the division Deuteromycota (Fungi Imperfecti), family Cryptococcaceae, were identified as two Rhodotorula rubra and one Torulopsis candida. It was demonstrated that the number of colonies isolated in the areas studied increased after the performance of technical maneuvers and that air filtration by means of HEPA filters, reducing to number of colonies isolated in restricted areas, increased safety and is consequently recommended for working areas where successful or unsuccessful results depend on a low incidence of contamination. Objetivou-se isolar e identificar a microbiota fúngica em ambientes considerados assépticos, através de exposições com meios de cultivo adequados, em três épocas distintas do ano, antes e imediatamente após as manobras técnicas realizadas em três áreas de trabalho: ambiente aberto, ambiente fechado sem filtração de ar e ambiente fechado com filtração de ar, utilizadas em produção de imunobiológicos. Os meios ágar-Sabouraud e ágar-soja, enriquecidos com 0,2% de extrato de levedura e sem cloranfenicol, foram estudados quanto à sua eficácia no isolamento de bolores e leveduras, considerando-se o número de colônias desenvolvidas e a freqüência dos diversos fungos isolados. Isolaram-se 67 espécimens, sendo 64 fungos filamentosos (bolores) e três leveduras. Dos bolores, 54 pertenciam a 22 gêneros da divisão Deuteromycota, famílias Moniliaceae e Dematiaceae, cinco amostras filamentosas foram incluídas na ordem Agonomycetales (Mycelia Sterilia), e uma amostra foi classificada na divisão Deuteromycota, ordem Sphaeropsidales, classe Coelomycetes. Da divisão Zygomycota, ordem Mucorales, família Mucoraceae, um único mucoráceo foi identificado até gênero. As três leveduras pertenciam também à divisão Deuteromycota (Fungi Imperfecti), família Cryptococcaceae, e foram identificadas como sendo duas Rhodotorula rubra e uma Torulopsis candida. Comprovou-se que o número de colônias isoladas aumentou após a realização das monobras técnicas e que a filtração de ar através de filtros tipo HEPA, reduzindo o número de colônias isoladas nos ambientes fechados, aumenta a segurança do trabalho; comumente é recomendada para áreas de atividade técnica cujos resultados satisfatórios estão diretamente relacionados com uma baixa incidência de contaminantes. Universidade de São Paulo. Faculdade de Saúde Pública1988-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/2349510.1590/S0034-89101988000300006Revista de Saúde Pública; Vol. 22 No. 3 (1988); 201-206 Revista de Saúde Pública; Vol. 22 Núm. 3 (1988); 201-206 Revista de Saúde Pública; v. 22 n. 3 (1988); 201-206 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/23495/25532Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessAlmeida, Maria Elisabete Sbrogio deMartini, Maria HelenaPorto, EdwardCamargo, Ana Maria Martins deRizzo, Edda deLacaz, Carlos da Silva2012-05-28T16:54:38Zoai:revistas.usp.br:article/23495Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-05-28T16:54:38Revista de Saúde Pública - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Identification of the fungic microbiota isolated in areas considered aseptic Identificação da microbiota fúngica de ambientes considerados assépticos |
title |
Identification of the fungic microbiota isolated in areas considered aseptic |
spellingShingle |
Identification of the fungic microbiota isolated in areas considered aseptic Almeida, Maria Elisabete Sbrogio de Fungos^i1^sisolame Leveduras^i1^sisolame Ambiente construído Assepsia Microbiologia ambiental Fungs^i2^sisolat Yeasts^i2^sisolat Environment controlled Asepsis Environmental microbiology |
title_short |
Identification of the fungic microbiota isolated in areas considered aseptic |
title_full |
Identification of the fungic microbiota isolated in areas considered aseptic |
title_fullStr |
Identification of the fungic microbiota isolated in areas considered aseptic |
title_full_unstemmed |
Identification of the fungic microbiota isolated in areas considered aseptic |
title_sort |
Identification of the fungic microbiota isolated in areas considered aseptic |
author |
Almeida, Maria Elisabete Sbrogio de |
author_facet |
Almeida, Maria Elisabete Sbrogio de Martini, Maria Helena Porto, Edward Camargo, Ana Maria Martins de Rizzo, Edda de Lacaz, Carlos da Silva |
author_role |
author |
author2 |
Martini, Maria Helena Porto, Edward Camargo, Ana Maria Martins de Rizzo, Edda de Lacaz, Carlos da Silva |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Almeida, Maria Elisabete Sbrogio de Martini, Maria Helena Porto, Edward Camargo, Ana Maria Martins de Rizzo, Edda de Lacaz, Carlos da Silva |
dc.subject.por.fl_str_mv |
Fungos^i1^sisolame Leveduras^i1^sisolame Ambiente construído Assepsia Microbiologia ambiental Fungs^i2^sisolat Yeasts^i2^sisolat Environment controlled Asepsis Environmental microbiology |
topic |
Fungos^i1^sisolame Leveduras^i1^sisolame Ambiente construído Assepsia Microbiologia ambiental Fungs^i2^sisolat Yeasts^i2^sisolat Environment controlled Asepsis Environmental microbiology |
description |
The isolation and identification of the fungic microbiota present in areas considered aseptic (open and/or restricted, with or without air filtration (HEPA filters) where production of immunobiologicals is carried out)were investigated Three exposures were made with appropriate culture media, in different seasons of the year, before and immediately after the performance of the technical work. Agar-Sabouraud and agar-soy media supplemented with 0.2% yeast extract and without chloranphenicol had their efficacy tests in isolating the filamentous fungia e (molds) and yeasts in the light of the number of cultures grown and the frequency exhibited by the various specimens isolated. Sixty-seven specimens (64 of filamentous fungiae (molds) and three yeasts) have been identified. Of the filamentous fungiae (molds), 54 belonged to 22 genera of the division Deuteromycota, families Moniliaceae and Dematiaceae, five were included in the order Agonomycetales (Mycelia Sterilia), and one was classified in the order Sphaeropsidales, class Coelomycetes. Of the division Zygomycota, order Mucorales, family Mucoraceae, only one specimen could be identified as to genus. The three yeasts, belonging also to the division Deuteromycota (Fungi Imperfecti), family Cryptococcaceae, were identified as two Rhodotorula rubra and one Torulopsis candida. It was demonstrated that the number of colonies isolated in the areas studied increased after the performance of technical maneuvers and that air filtration by means of HEPA filters, reducing to number of colonies isolated in restricted areas, increased safety and is consequently recommended for working areas where successful or unsuccessful results depend on a low incidence of contamination. |
publishDate |
1988 |
dc.date.none.fl_str_mv |
1988-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/23495 10.1590/S0034-89101988000300006 |
url |
https://www.revistas.usp.br/rsp/article/view/23495 |
identifier_str_mv |
10.1590/S0034-89101988000300006 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/23495/25532 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Revista de Saúde Pública info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Revista de Saúde Pública |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
dc.source.none.fl_str_mv |
Revista de Saúde Pública; Vol. 22 No. 3 (1988); 201-206 Revista de Saúde Pública; Vol. 22 Núm. 3 (1988); 201-206 Revista de Saúde Pública; v. 22 n. 3 (1988); 201-206 1518-8787 0034-8910 reponame:Revista de Saúde Pública instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Revista de Saúde Pública |
collection |
Revista de Saúde Pública |
repository.name.fl_str_mv |
Revista de Saúde Pública - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
revsp@org.usp.br||revsp1@usp.br |
_version_ |
1800221774020870144 |