Susceptibility of Vero cell line to vaccine strains of the measles virus
Autor(a) principal: | |
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Data de Publicação: | 1994 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Saúde Pública |
Texto Completo: | https://www.revistas.usp.br/rsp/article/view/24035 |
Resumo: | Vero cells used by distinct measles vaccine control laboratories had their susceptibility to Moraten, Schwarz and Biken CAM-70 vaccine strains assayed. Of a total of 72 lots of measles vaccine whose potency was titrated by microtechnique in two Vero cell samples (Vero IB and Vero INCQS), 25 had been produced with Moraten strain, 24 with Schwarz and 23 with Biken CAM-70. The statistical analysis of the results demonstrated that both Vero cells assayed presented comparable susceptibility to Moraten and Biken CAM-70 strains. As to the Schwarz strain, Vero IB cells were more susceptible than the other cell sample tested, thus confirming the existence of different sensitivities of Vero cells to some measles vaccine strains, or even to viruses derived from the same strain but with different passage histories. An altered cell susceptibility to virus replication may significantly alter the results in potency testing. Such alteration may be caused not only by the adoption of distinct protocols for the maintenance of cell cultures by different control laboratories but also by the methodology followed in the vaccine titration. In order to minimize the differences existing among the results obtained in the potency testing, it is suggested that all control laboratories should use the same protocols for cell culture maintenance as well as for vaccine potency testing. |
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Susceptibility of Vero cell line to vaccine strains of the measles virus Suscetibilidade da linhagem de células Vero a cepas vacinais do vírus do sarampo Vacina contra sarampo^i1^snorVírus do sarampo^i1^simunoloSuscetibilidade celularMeasles vaccine^i2^sstandaMeasles virus^i2^simmunolCell susceptibility Vero cells used by distinct measles vaccine control laboratories had their susceptibility to Moraten, Schwarz and Biken CAM-70 vaccine strains assayed. Of a total of 72 lots of measles vaccine whose potency was titrated by microtechnique in two Vero cell samples (Vero IB and Vero INCQS), 25 had been produced with Moraten strain, 24 with Schwarz and 23 with Biken CAM-70. The statistical analysis of the results demonstrated that both Vero cells assayed presented comparable susceptibility to Moraten and Biken CAM-70 strains. As to the Schwarz strain, Vero IB cells were more susceptible than the other cell sample tested, thus confirming the existence of different sensitivities of Vero cells to some measles vaccine strains, or even to viruses derived from the same strain but with different passage histories. An altered cell susceptibility to virus replication may significantly alter the results in potency testing. Such alteration may be caused not only by the adoption of distinct protocols for the maintenance of cell cultures by different control laboratories but also by the methodology followed in the vaccine titration. In order to minimize the differences existing among the results obtained in the potency testing, it is suggested that all control laboratories should use the same protocols for cell culture maintenance as well as for vaccine potency testing. A suscetibilidade da linhagem de células Vero ao vírus do sarampo é bem conhecida e sua utilização no controle da potência da vacina contra o sarampo é amplamente difundida. Com o objetivo de comparar a suscetibilidade de células Vero empregadas em titulações, amostras provenientes de dois laboratórios controladores (Vero IB e Vero INCQS), foram testadas frente a três cepas vacinais: Moraten, Schwarz e Biken CAM-70. Foram titulados 72 lotes de vacinas contra o sarampo, sendo 25 produzidos com a cepa Moraten, 24 com a cepa Schwarz e 23 com a cepa Biken CAM-70. A análise estatística dos resultados obtidos nas titulações, feita através dos testes Limites para uma Média e "t" de Student, mostrou que para as cepas Moraten e Biken CAM-70, as diferenças de títulos não foram estatisticamente significantes, o mesmo não ocorrendo com a cepa Schwarz, para a qual as células Vero IB se mostraram mais sensíveis. Universidade de São Paulo. Faculdade de Saúde Pública1994-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/2403510.1590/S0034-89101994000300008Revista de Saúde Pública; Vol. 28 No. 3 (1994); 209-212 Revista de Saúde Pública; Vol. 28 Núm. 3 (1994); 209-212 Revista de Saúde Pública; v. 28 n. 3 (1994); 209-212 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/24035/26000Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessTakata, Célia SayokoKubrusly, Flávia SaldanhaMiyaki, CosueMendes, Inácio FrançaRizzo, Edda de2012-05-29T16:19:32Zoai:revistas.usp.br:article/24035Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-05-29T16:19:32Revista de Saúde Pública - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Susceptibility of Vero cell line to vaccine strains of the measles virus Suscetibilidade da linhagem de células Vero a cepas vacinais do vírus do sarampo |
title |
Susceptibility of Vero cell line to vaccine strains of the measles virus |
spellingShingle |
Susceptibility of Vero cell line to vaccine strains of the measles virus Takata, Célia Sayoko Vacina contra sarampo^i1^snor Vírus do sarampo^i1^simunolo Suscetibilidade celular Measles vaccine^i2^sstanda Measles virus^i2^simmunol Cell susceptibility |
title_short |
Susceptibility of Vero cell line to vaccine strains of the measles virus |
title_full |
Susceptibility of Vero cell line to vaccine strains of the measles virus |
title_fullStr |
Susceptibility of Vero cell line to vaccine strains of the measles virus |
title_full_unstemmed |
Susceptibility of Vero cell line to vaccine strains of the measles virus |
title_sort |
Susceptibility of Vero cell line to vaccine strains of the measles virus |
author |
Takata, Célia Sayoko |
author_facet |
Takata, Célia Sayoko Kubrusly, Flávia Saldanha Miyaki, Cosue Mendes, Inácio França Rizzo, Edda de |
author_role |
author |
author2 |
Kubrusly, Flávia Saldanha Miyaki, Cosue Mendes, Inácio França Rizzo, Edda de |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Takata, Célia Sayoko Kubrusly, Flávia Saldanha Miyaki, Cosue Mendes, Inácio França Rizzo, Edda de |
dc.subject.por.fl_str_mv |
Vacina contra sarampo^i1^snor Vírus do sarampo^i1^simunolo Suscetibilidade celular Measles vaccine^i2^sstanda Measles virus^i2^simmunol Cell susceptibility |
topic |
Vacina contra sarampo^i1^snor Vírus do sarampo^i1^simunolo Suscetibilidade celular Measles vaccine^i2^sstanda Measles virus^i2^simmunol Cell susceptibility |
description |
Vero cells used by distinct measles vaccine control laboratories had their susceptibility to Moraten, Schwarz and Biken CAM-70 vaccine strains assayed. Of a total of 72 lots of measles vaccine whose potency was titrated by microtechnique in two Vero cell samples (Vero IB and Vero INCQS), 25 had been produced with Moraten strain, 24 with Schwarz and 23 with Biken CAM-70. The statistical analysis of the results demonstrated that both Vero cells assayed presented comparable susceptibility to Moraten and Biken CAM-70 strains. As to the Schwarz strain, Vero IB cells were more susceptible than the other cell sample tested, thus confirming the existence of different sensitivities of Vero cells to some measles vaccine strains, or even to viruses derived from the same strain but with different passage histories. An altered cell susceptibility to virus replication may significantly alter the results in potency testing. Such alteration may be caused not only by the adoption of distinct protocols for the maintenance of cell cultures by different control laboratories but also by the methodology followed in the vaccine titration. In order to minimize the differences existing among the results obtained in the potency testing, it is suggested that all control laboratories should use the same protocols for cell culture maintenance as well as for vaccine potency testing. |
publishDate |
1994 |
dc.date.none.fl_str_mv |
1994-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/24035 10.1590/S0034-89101994000300008 |
url |
https://www.revistas.usp.br/rsp/article/view/24035 |
identifier_str_mv |
10.1590/S0034-89101994000300008 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/24035/26000 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Revista de Saúde Pública info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Revista de Saúde Pública |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
dc.source.none.fl_str_mv |
Revista de Saúde Pública; Vol. 28 No. 3 (1994); 209-212 Revista de Saúde Pública; Vol. 28 Núm. 3 (1994); 209-212 Revista de Saúde Pública; v. 28 n. 3 (1994); 209-212 1518-8787 0034-8910 reponame:Revista de Saúde Pública instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Revista de Saúde Pública |
collection |
Revista de Saúde Pública |
repository.name.fl_str_mv |
Revista de Saúde Pública - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
revsp@org.usp.br||revsp1@usp.br |
_version_ |
1800221776984145920 |