The use of a semipurified fraction of Trypanosoma cruzi proteinase in immunodiagnosis of Chagas' disease

Detalhes bibliográficos
Autor(a) principal: Atta, Ajax Mercês
Data de Publicação: 1984
Outros Autores: Pontes, Angela Maria de Carvalho, Souza, Maria Luiza de, Repka, Daria, Rangel, Humberto A.
Tipo de documento: Artigo
Idioma: por
Título da fonte: Revista de Saúde Pública
Texto Completo: https://www.revistas.usp.br/rsp/article/view/23259
Resumo: Group 0 Rh negative human erytrocytes were coated with the semipurified fraction of T. cruzi proteinase and tested with sera both from patients with chagas' disease and from others with unrelated parasitic diseases. Positive haemagglutination reactions were only observed with the sera from the former and with that from two patients with mucocutaneous leishmaniasis. No crossed reactions were observed with visceral leishmaniasis, malaria, toxoplasmosis syphilis, schistosomiasis or mononucleosis sera. Results suggest that this purified fraction can be used in immunodiagnosis of American Trypanosomiasis.
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spelling The use of a semipurified fraction of Trypanosoma cruzi proteinase in immunodiagnosis of Chagas' disease Utilização da fração semipurificada da proteinase do Trypanosoma cruzi no imunodiagnóstico da doença de Chagas Tripanossomíase americana^i1^sdiagnóstTrypanosoma cruziProteinaseTrypanosomiasisSouth American^i2^sdiagnoTrypanosoma cruziPeptide peptidohydrolases Group 0 Rh negative human erytrocytes were coated with the semipurified fraction of T. cruzi proteinase and tested with sera both from patients with chagas' disease and from others with unrelated parasitic diseases. Positive haemagglutination reactions were only observed with the sera from the former and with that from two patients with mucocutaneous leishmaniasis. No crossed reactions were observed with visceral leishmaniasis, malaria, toxoplasmosis syphilis, schistosomiasis or mononucleosis sera. Results suggest that this purified fraction can be used in immunodiagnosis of American Trypanosomiasis. Foram sensibilizadas hemácias humanas 0 Rh negativo com a fração semipurificada (Fp) da proteinase do Trypanosoma cruzi, e testadas quanto a antigenicidade com soros de pacientes portadores de tripanossomíase americana crônica e de outras doenças parasitárias não relacionadas. Reações de hemaglutinação positivas foram observadas com os soros de pacientes chagásicos e com alguns soros de indivíduos portadores de leishmaniose cutaneo-mucosa. Não foram observadas reações cruzadas com os soros de pacientes portadores de leishmaniose visceral, malária, toxoplasmose, sífilis, esquistossomose e mononucleose. Os resultados obtidos são favoráveis ao emprego desta fração antigênica em testes de imunodiagnóstico da tripanossomíase americana. Universidade de São Paulo. Faculdade de Saúde Pública1984-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/2325910.1590/S0034-89101984000600007Revista de Saúde Pública; Vol. 18 No. 6 (1984); 487-490 Revista de Saúde Pública; Vol. 18 Núm. 6 (1984); 487-490 Revista de Saúde Pública; v. 18 n. 6 (1984); 487-490 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/23259/25287Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessAtta, Ajax MercêsPontes, Angela Maria de CarvalhoSouza, Maria Luiza deRepka, DariaRangel, Humberto A.2012-05-28T15:22:12Zoai:revistas.usp.br:article/23259Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-05-28T15:22:12Revista de Saúde Pública - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv The use of a semipurified fraction of Trypanosoma cruzi proteinase in immunodiagnosis of Chagas' disease
Utilização da fração semipurificada da proteinase do Trypanosoma cruzi no imunodiagnóstico da doença de Chagas
title The use of a semipurified fraction of Trypanosoma cruzi proteinase in immunodiagnosis of Chagas' disease
spellingShingle The use of a semipurified fraction of Trypanosoma cruzi proteinase in immunodiagnosis of Chagas' disease
Atta, Ajax Mercês
Tripanossomíase americana^i1^sdiagnóst
Trypanosoma cruzi
Proteinase
Trypanosomiasis
South American^i2^sdiagno
Trypanosoma cruzi
Peptide peptidohydrolases
title_short The use of a semipurified fraction of Trypanosoma cruzi proteinase in immunodiagnosis of Chagas' disease
title_full The use of a semipurified fraction of Trypanosoma cruzi proteinase in immunodiagnosis of Chagas' disease
title_fullStr The use of a semipurified fraction of Trypanosoma cruzi proteinase in immunodiagnosis of Chagas' disease
title_full_unstemmed The use of a semipurified fraction of Trypanosoma cruzi proteinase in immunodiagnosis of Chagas' disease
title_sort The use of a semipurified fraction of Trypanosoma cruzi proteinase in immunodiagnosis of Chagas' disease
author Atta, Ajax Mercês
author_facet Atta, Ajax Mercês
Pontes, Angela Maria de Carvalho
Souza, Maria Luiza de
Repka, Daria
Rangel, Humberto A.
author_role author
author2 Pontes, Angela Maria de Carvalho
Souza, Maria Luiza de
Repka, Daria
Rangel, Humberto A.
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Atta, Ajax Mercês
Pontes, Angela Maria de Carvalho
Souza, Maria Luiza de
Repka, Daria
Rangel, Humberto A.
dc.subject.por.fl_str_mv Tripanossomíase americana^i1^sdiagnóst
Trypanosoma cruzi
Proteinase
Trypanosomiasis
South American^i2^sdiagno
Trypanosoma cruzi
Peptide peptidohydrolases
topic Tripanossomíase americana^i1^sdiagnóst
Trypanosoma cruzi
Proteinase
Trypanosomiasis
South American^i2^sdiagno
Trypanosoma cruzi
Peptide peptidohydrolases
description Group 0 Rh negative human erytrocytes were coated with the semipurified fraction of T. cruzi proteinase and tested with sera both from patients with chagas' disease and from others with unrelated parasitic diseases. Positive haemagglutination reactions were only observed with the sera from the former and with that from two patients with mucocutaneous leishmaniasis. No crossed reactions were observed with visceral leishmaniasis, malaria, toxoplasmosis syphilis, schistosomiasis or mononucleosis sera. Results suggest that this purified fraction can be used in immunodiagnosis of American Trypanosomiasis.
publishDate 1984
dc.date.none.fl_str_mv 1984-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/rsp/article/view/23259
10.1590/S0034-89101984000600007
url https://www.revistas.usp.br/rsp/article/view/23259
identifier_str_mv 10.1590/S0034-89101984000600007
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/rsp/article/view/23259/25287
dc.rights.driver.fl_str_mv Copyright (c) 2017 Revista de Saúde Pública
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2017 Revista de Saúde Pública
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Saúde Pública
dc.source.none.fl_str_mv Revista de Saúde Pública; Vol. 18 No. 6 (1984); 487-490
Revista de Saúde Pública; Vol. 18 Núm. 6 (1984); 487-490
Revista de Saúde Pública; v. 18 n. 6 (1984); 487-490
1518-8787
0034-8910
reponame:Revista de Saúde Pública
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista de Saúde Pública
collection Revista de Saúde Pública
repository.name.fl_str_mv Revista de Saúde Pública - Universidade de São Paulo (USP)
repository.mail.fl_str_mv revsp@org.usp.br||revsp1@usp.br
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