Blue rayon and Salmonella/microsome assay in the evaluation of coastal water quality
Autor(a) principal: | |
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Data de Publicação: | 2006 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Revista de Saúde Pública |
Texto Completo: | https://www.revistas.usp.br/rsp/article/view/32149 |
Resumo: | OBJECTIVE: To develop a strategy for water quality monitoring for the presence of genotoxins and polycyclic aromatic hydrocarbons. METHODS: A study was carried out in Santos estuary, Southern Brazil, in 2002. Two sampling sites with different concentration levels were selected and evaluated in different samplings using blue rayon hanging technique, chemical analyses, and Salmonella/microsome assay with bacterial strains sensitive to different compounds. The extracts were tested using the Salmonella/microsome assay in microsuspension with the strains TA98, TA100, YG1041, and YG1042 in the absence and presence of metabolic activation and through chemical analyses. RESULTS: Site 1, which had high concentrations of polycyclic aromatic hydrocarbons in its sediment, showed more often positive results in the Salmonella/microsome assay as well as higher polycyclic aromatic hydrocarbons concentrations in both samplings compared to site 2, which was less contaminated. YG1041 strain showed to be the most sensitive allowing for comparisons between the sites with different levels of contamination. CONCLUSIONS: The combination of the blue rayon hanging technique with the Salmonella/microsome assay using YG1041 strain and chemical analyses were effective in recovering genotoxins as well as polycyclic aromatic hydrocarbons tested in this study. Therefore this strategy seems to be adequate for water quality monitoring in Santos estuary. |
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Blue rayon and Salmonella/microsome assay in the evaluation of coastal water quality Blue rayon e teste Salmonella/microssoma na avaliação da qualidade de águas costeiras Poluição dos estuários^i1^sprevenção e contrMonitoramento da águaHidrocarbonetos policíclicos aromáticos^i1^sgenotoxicidBlue rayonSalmonella/microssoma^i1^smonitorameEstuário de SantosEstuary pollution^i2^sprevention & contWater monitoringPolycyclic hydrocarbonsaromatic^i2^sgenotoxicBlue rayonSalmonella/microsome^i2^smonitorSantos estuary OBJECTIVE: To develop a strategy for water quality monitoring for the presence of genotoxins and polycyclic aromatic hydrocarbons. METHODS: A study was carried out in Santos estuary, Southern Brazil, in 2002. Two sampling sites with different concentration levels were selected and evaluated in different samplings using blue rayon hanging technique, chemical analyses, and Salmonella/microsome assay with bacterial strains sensitive to different compounds. The extracts were tested using the Salmonella/microsome assay in microsuspension with the strains TA98, TA100, YG1041, and YG1042 in the absence and presence of metabolic activation and through chemical analyses. RESULTS: Site 1, which had high concentrations of polycyclic aromatic hydrocarbons in its sediment, showed more often positive results in the Salmonella/microsome assay as well as higher polycyclic aromatic hydrocarbons concentrations in both samplings compared to site 2, which was less contaminated. YG1041 strain showed to be the most sensitive allowing for comparisons between the sites with different levels of contamination. CONCLUSIONS: The combination of the blue rayon hanging technique with the Salmonella/microsome assay using YG1041 strain and chemical analyses were effective in recovering genotoxins as well as polycyclic aromatic hydrocarbons tested in this study. Therefore this strategy seems to be adequate for water quality monitoring in Santos estuary. OBJETIVO: Desenvolver estratégia para o monitoramento passivo das águas do estuário de Santos quanto à presença de atividade genotóxica e de hidrocarbonetos policíclicos aromáticos. MÉTODOS: Estudo realizado no estuário de Santos, Estado de São Paulo, em 2002. Foram selecionados e avaliados dois pontos de amostragem com diferentes graus de contaminação em duas campanhas de amostragem, utilizando a técnica de blue rayon in situ, análises químicas e o ensaio de Salmonella/microssoma com as linhagens bacterianas sensíveis a diferentes classes de compostos. Os extratos foram submetidos ao teste de Salmonella/microssoma em microssuspensão com as linhagens TA98, TA100, YG1041 e YG1042 na presença e ausência de ativação metabólica, e a análises químicas. RESULTADOS: O ponto 1, que apresentou sedimento com altas concentrações de hidrocarbonetos policíclicos aromáticos, mostrou maior freqüência de resultados positivos para o ensaio Samonella/microssoma e maiores concentrações de hidrocarbonetos policíclicos aromáticos em ambas as campanhas em comparação com o ponto 2, menos contaminado. A linhagem que se mostrou mais sensível foi a YG1041, que permitiu comparações entre locais com diferentes graus de contaminação. CONCLUSÕES: A combinação da técnica de blue rayon in situ com o ensaio Salmonella/microsoma com a linhagem YG1041 e as análises químicas se mostraram eficientes. Foi possível recuperar os compostos genotóxicos, e os hidrocarbonetos policíclicos aromáticos analisados, parecendo ser uma estratégia adequada para o monitoramento da qualidade das águas do estuário de Santos. Universidade de São Paulo. Faculdade de Saúde Pública2006-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/3214910.1590/S0034-89102006000600020Revista de Saúde Pública; Vol. 40 No. 5 (2006); 890-897 Revista de Saúde Pública; Vol. 40 Núm. 5 (2006); 890-897 Revista de Saúde Pública; v. 40 n. 5 (2006); 890-897 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporhttps://www.revistas.usp.br/rsp/article/view/32149/34236Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessKummrow, FábioMagalhães, DulceFranco, AlexandreUmbuzeiro, Gisela de Aragão2012-07-08T23:15:10Zoai:revistas.usp.br:article/32149Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-07-08T23:15:10Revista de Saúde Pública - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Blue rayon and Salmonella/microsome assay in the evaluation of coastal water quality Blue rayon e teste Salmonella/microssoma na avaliação da qualidade de águas costeiras |
title |
Blue rayon and Salmonella/microsome assay in the evaluation of coastal water quality |
spellingShingle |
Blue rayon and Salmonella/microsome assay in the evaluation of coastal water quality Kummrow, Fábio Poluição dos estuários^i1^sprevenção e contr Monitoramento da água Hidrocarbonetos policíclicos aromáticos^i1^sgenotoxicid Blue rayon Salmonella/microssoma^i1^smonitorame Estuário de Santos Estuary pollution^i2^sprevention & cont Water monitoring Polycyclic hydrocarbons aromatic^i2^sgenotoxic Blue rayon Salmonella/microsome^i2^smonitor Santos estuary |
title_short |
Blue rayon and Salmonella/microsome assay in the evaluation of coastal water quality |
title_full |
Blue rayon and Salmonella/microsome assay in the evaluation of coastal water quality |
title_fullStr |
Blue rayon and Salmonella/microsome assay in the evaluation of coastal water quality |
title_full_unstemmed |
Blue rayon and Salmonella/microsome assay in the evaluation of coastal water quality |
title_sort |
Blue rayon and Salmonella/microsome assay in the evaluation of coastal water quality |
author |
Kummrow, Fábio |
author_facet |
Kummrow, Fábio Magalhães, Dulce Franco, Alexandre Umbuzeiro, Gisela de Aragão |
author_role |
author |
author2 |
Magalhães, Dulce Franco, Alexandre Umbuzeiro, Gisela de Aragão |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Kummrow, Fábio Magalhães, Dulce Franco, Alexandre Umbuzeiro, Gisela de Aragão |
dc.subject.por.fl_str_mv |
Poluição dos estuários^i1^sprevenção e contr Monitoramento da água Hidrocarbonetos policíclicos aromáticos^i1^sgenotoxicid Blue rayon Salmonella/microssoma^i1^smonitorame Estuário de Santos Estuary pollution^i2^sprevention & cont Water monitoring Polycyclic hydrocarbons aromatic^i2^sgenotoxic Blue rayon Salmonella/microsome^i2^smonitor Santos estuary |
topic |
Poluição dos estuários^i1^sprevenção e contr Monitoramento da água Hidrocarbonetos policíclicos aromáticos^i1^sgenotoxicid Blue rayon Salmonella/microssoma^i1^smonitorame Estuário de Santos Estuary pollution^i2^sprevention & cont Water monitoring Polycyclic hydrocarbons aromatic^i2^sgenotoxic Blue rayon Salmonella/microsome^i2^smonitor Santos estuary |
description |
OBJECTIVE: To develop a strategy for water quality monitoring for the presence of genotoxins and polycyclic aromatic hydrocarbons. METHODS: A study was carried out in Santos estuary, Southern Brazil, in 2002. Two sampling sites with different concentration levels were selected and evaluated in different samplings using blue rayon hanging technique, chemical analyses, and Salmonella/microsome assay with bacterial strains sensitive to different compounds. The extracts were tested using the Salmonella/microsome assay in microsuspension with the strains TA98, TA100, YG1041, and YG1042 in the absence and presence of metabolic activation and through chemical analyses. RESULTS: Site 1, which had high concentrations of polycyclic aromatic hydrocarbons in its sediment, showed more often positive results in the Salmonella/microsome assay as well as higher polycyclic aromatic hydrocarbons concentrations in both samplings compared to site 2, which was less contaminated. YG1041 strain showed to be the most sensitive allowing for comparisons between the sites with different levels of contamination. CONCLUSIONS: The combination of the blue rayon hanging technique with the Salmonella/microsome assay using YG1041 strain and chemical analyses were effective in recovering genotoxins as well as polycyclic aromatic hydrocarbons tested in this study. Therefore this strategy seems to be adequate for water quality monitoring in Santos estuary. |
publishDate |
2006 |
dc.date.none.fl_str_mv |
2006-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/32149 10.1590/S0034-89102006000600020 |
url |
https://www.revistas.usp.br/rsp/article/view/32149 |
identifier_str_mv |
10.1590/S0034-89102006000600020 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/32149/34236 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Revista de Saúde Pública info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Revista de Saúde Pública |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
dc.source.none.fl_str_mv |
Revista de Saúde Pública; Vol. 40 No. 5 (2006); 890-897 Revista de Saúde Pública; Vol. 40 Núm. 5 (2006); 890-897 Revista de Saúde Pública; v. 40 n. 5 (2006); 890-897 1518-8787 0034-8910 reponame:Revista de Saúde Pública instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Revista de Saúde Pública |
collection |
Revista de Saúde Pública |
repository.name.fl_str_mv |
Revista de Saúde Pública - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
revsp@org.usp.br||revsp1@usp.br |
_version_ |
1800221785345490944 |