Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil
Autor(a) principal: | |
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Data de Publicação: | 2007 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Revista de Saúde Pública |
Texto Completo: | https://www.revistas.usp.br/rsp/article/view/32242 |
Resumo: | OBJECTIVE: To identify the species of bats involved in maintaining the rabies cycle; to investigate the distribution of the rabies virus in the tissues and organs of bats and the time taken for mortality among inoculated mice. METHODS: From April 2002 to November 2003, bats from municipalities in the State of São Paulo were screened for the presence of the rabies virus, by means of direct immunofluorescence. The virus distribution in the bats was evaluated by inoculating mice and N2A cells with 20% suspensions prepared from fragments of different organs and tissues, plus the brain and salivary glands. The time taken for mortality among the mice was monitored daily, following intracerebral inoculation. RESULTS: Out of the 4,395 bats received, 1.9% were found positive for the rabies virus. They belonged to ten genera, with predominance of insectivores. The maximum mean times taken for mortality among the mice following inoculation with brain and salivary gland material were 15.33±2.08 days and 11.33±2.30 days for vampire bats, 16.45±4.48 days and 18.91±6.12 days for insectivorous bats, and 12.60±2.13 days and 15.67±4.82 days for frugivorous bats, respectively. CONCLUSIONS: The species infected with the rabies virus were: Artibeus lituratus, Artibeus sp., Myotis nigricans, Myotis sp., Eptesicus sp., Lasiurus ega, Lasiurus cinereus, Nyctinomops laticaudatus, Tadarida brasiliensis, Histiotus velatus, Molossus rufus, Eumops sp. and Desmodus rotundus. Virus investigation in the different tissues and organs showed that the brain and salivary glands were the most suitable sites for virus isolation. |
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Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil Vírus da raiva em quirópteros naturalmente infectados no Estado de São Paulo, Brasil Vírus da raiva^i1^sisolamento e purificaRaiva^i1^sviroloQuirópteros^i1^sviroloCamundongos^i1^sviroloTécnicas de cultura de célulasRabies vírus^i2^sisolation & purificatRabies^i2^svirolChiroptera^i2^svirolMice^i2^svirolCell culture techniques OBJECTIVE: To identify the species of bats involved in maintaining the rabies cycle; to investigate the distribution of the rabies virus in the tissues and organs of bats and the time taken for mortality among inoculated mice. METHODS: From April 2002 to November 2003, bats from municipalities in the State of São Paulo were screened for the presence of the rabies virus, by means of direct immunofluorescence. The virus distribution in the bats was evaluated by inoculating mice and N2A cells with 20% suspensions prepared from fragments of different organs and tissues, plus the brain and salivary glands. The time taken for mortality among the mice was monitored daily, following intracerebral inoculation. RESULTS: Out of the 4,395 bats received, 1.9% were found positive for the rabies virus. They belonged to ten genera, with predominance of insectivores. The maximum mean times taken for mortality among the mice following inoculation with brain and salivary gland material were 15.33±2.08 days and 11.33±2.30 days for vampire bats, 16.45±4.48 days and 18.91±6.12 days for insectivorous bats, and 12.60±2.13 days and 15.67±4.82 days for frugivorous bats, respectively. CONCLUSIONS: The species infected with the rabies virus were: Artibeus lituratus, Artibeus sp., Myotis nigricans, Myotis sp., Eptesicus sp., Lasiurus ega, Lasiurus cinereus, Nyctinomops laticaudatus, Tadarida brasiliensis, Histiotus velatus, Molossus rufus, Eumops sp. and Desmodus rotundus. Virus investigation in the different tissues and organs showed that the brain and salivary glands were the most suitable sites for virus isolation. OBJETIVO: Identificar as espécies de morcegos envolvidas na manutenção do ciclo da raiva, verificar a distribuição do vírus da raiva em tecidos e órgãos de morcegos e os períodos de mortalidade dos camundongos inoculados. MÉTODOS: A positividade para o vírus da raiva foi avaliada por imunofluorescência direta em morcegos de municípios do Estado de São Paulo, de abril de 2002 a novembro de 2003. A distribuição do vírus nos morcegos foi avaliada pela inoculação de camundongos e infecção de células N2A, com suspensões a 20% preparadas a partir de fragmentos de diversos órgãos e tecidos, além de cérebro e glândula salivar. A mortalidade dos camundongos foi observada diariamente, após inoculação intracerebral. RESULTADOS: Dos 4.393 morcegos pesquisados, 1,9% foram positivos para o vírus da raiva, pertencentes a dez gêneros, com predomínio de insetívoros. A média do período máximo de mortalidade dos camundongos pós-inoculação a partir de cérebros e glândulas salivares de morcegos hematófagos foi de 15,33±2,08 dias e 11,33±2,30 dias; insetívoros, 16,45±4,48 dias e 18,91±6,12 dias; e frugívoros, 12,60±2,13 dias e 15,67±4,82 dias, respectivamente. CONCLUSÕES: As espécies infectadas com o vírus da raiva foram: Artibeus lituratus, Artibeus sp., Myotis nigricans, Myotis sp., Eptesicus sp., Lasiurus ega, Lasiurus cinereus, Nyctinomops laticaudatus, Tadarida brasiliensis, Histiotus velatus, Molossus rufus, Eumops sp. e Desmodus rotundus. A pesquisa de vírus em diferentes tecidos e órgãos mostrou-se que os mais apropriados para o isolamento foram cérebro e glândulas salivares. Universidade de São Paulo. Faculdade de Saúde Pública2007-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://www.revistas.usp.br/rsp/article/view/3224210.1590/S0034-89102007000300010Revista de Saúde Pública; Vol. 41 No. 3 (2007); 389-395 Revista de Saúde Pública; Vol. 41 Núm. 3 (2007); 389-395 Revista de Saúde Pública; v. 41 n. 3 (2007); 389-395 1518-87870034-8910reponame:Revista de Saúde Públicainstname:Universidade de São Paulo (USP)instacron:USPporenghttps://www.revistas.usp.br/rsp/article/view/32242/34369https://www.revistas.usp.br/rsp/article/view/32242/34370Copyright (c) 2017 Revista de Saúde Públicainfo:eu-repo/semantics/openAccessScheffer, Karin CorrêaCarrieri, Maria LuizaAlbas, AvelinoSantos, Helaine Cristina Pires dosKotait, IvaneteIto, Fumio Honma2012-07-09T00:33:50Zoai:revistas.usp.br:article/32242Revistahttps://www.revistas.usp.br/rsp/indexONGhttps://www.revistas.usp.br/rsp/oairevsp@org.usp.br||revsp1@usp.br1518-87870034-8910opendoar:2012-07-09T00:33:50Revista de Saúde Pública - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil Vírus da raiva em quirópteros naturalmente infectados no Estado de São Paulo, Brasil |
title |
Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil |
spellingShingle |
Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil Scheffer, Karin Corrêa Vírus da raiva^i1^sisolamento e purifica Raiva^i1^svirolo Quirópteros^i1^svirolo Camundongos^i1^svirolo Técnicas de cultura de células Rabies vírus^i2^sisolation & purificat Rabies^i2^svirol Chiroptera^i2^svirol Mice^i2^svirol Cell culture techniques |
title_short |
Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil |
title_full |
Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil |
title_fullStr |
Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil |
title_full_unstemmed |
Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil |
title_sort |
Rabies virus in naturally infected bats in the State of São Paulo, Southeastern Brazil |
author |
Scheffer, Karin Corrêa |
author_facet |
Scheffer, Karin Corrêa Carrieri, Maria Luiza Albas, Avelino Santos, Helaine Cristina Pires dos Kotait, Ivanete Ito, Fumio Honma |
author_role |
author |
author2 |
Carrieri, Maria Luiza Albas, Avelino Santos, Helaine Cristina Pires dos Kotait, Ivanete Ito, Fumio Honma |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Scheffer, Karin Corrêa Carrieri, Maria Luiza Albas, Avelino Santos, Helaine Cristina Pires dos Kotait, Ivanete Ito, Fumio Honma |
dc.subject.por.fl_str_mv |
Vírus da raiva^i1^sisolamento e purifica Raiva^i1^svirolo Quirópteros^i1^svirolo Camundongos^i1^svirolo Técnicas de cultura de células Rabies vírus^i2^sisolation & purificat Rabies^i2^svirol Chiroptera^i2^svirol Mice^i2^svirol Cell culture techniques |
topic |
Vírus da raiva^i1^sisolamento e purifica Raiva^i1^svirolo Quirópteros^i1^svirolo Camundongos^i1^svirolo Técnicas de cultura de células Rabies vírus^i2^sisolation & purificat Rabies^i2^svirol Chiroptera^i2^svirol Mice^i2^svirol Cell culture techniques |
description |
OBJECTIVE: To identify the species of bats involved in maintaining the rabies cycle; to investigate the distribution of the rabies virus in the tissues and organs of bats and the time taken for mortality among inoculated mice. METHODS: From April 2002 to November 2003, bats from municipalities in the State of São Paulo were screened for the presence of the rabies virus, by means of direct immunofluorescence. The virus distribution in the bats was evaluated by inoculating mice and N2A cells with 20% suspensions prepared from fragments of different organs and tissues, plus the brain and salivary glands. The time taken for mortality among the mice was monitored daily, following intracerebral inoculation. RESULTS: Out of the 4,395 bats received, 1.9% were found positive for the rabies virus. They belonged to ten genera, with predominance of insectivores. The maximum mean times taken for mortality among the mice following inoculation with brain and salivary gland material were 15.33±2.08 days and 11.33±2.30 days for vampire bats, 16.45±4.48 days and 18.91±6.12 days for insectivorous bats, and 12.60±2.13 days and 15.67±4.82 days for frugivorous bats, respectively. CONCLUSIONS: The species infected with the rabies virus were: Artibeus lituratus, Artibeus sp., Myotis nigricans, Myotis sp., Eptesicus sp., Lasiurus ega, Lasiurus cinereus, Nyctinomops laticaudatus, Tadarida brasiliensis, Histiotus velatus, Molossus rufus, Eumops sp. and Desmodus rotundus. Virus investigation in the different tissues and organs showed that the brain and salivary glands were the most suitable sites for virus isolation. |
publishDate |
2007 |
dc.date.none.fl_str_mv |
2007-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/32242 10.1590/S0034-89102007000300010 |
url |
https://www.revistas.usp.br/rsp/article/view/32242 |
identifier_str_mv |
10.1590/S0034-89102007000300010 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/rsp/article/view/32242/34369 https://www.revistas.usp.br/rsp/article/view/32242/34370 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2017 Revista de Saúde Pública info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2017 Revista de Saúde Pública |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Saúde Pública |
dc.source.none.fl_str_mv |
Revista de Saúde Pública; Vol. 41 No. 3 (2007); 389-395 Revista de Saúde Pública; Vol. 41 Núm. 3 (2007); 389-395 Revista de Saúde Pública; v. 41 n. 3 (2007); 389-395 1518-8787 0034-8910 reponame:Revista de Saúde Pública instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Revista de Saúde Pública |
collection |
Revista de Saúde Pública |
repository.name.fl_str_mv |
Revista de Saúde Pública - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
revsp@org.usp.br||revsp1@usp.br |
_version_ |
1800221786103611392 |