Protective effect of sodium bicarbonate on radiological contrast medium-induced nephropathy in rats
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | por eng |
Título da fonte: | Revista da Escola de Enfermagem da USP (Online) |
Texto Completo: | https://www.revistas.usp.br/reeusp/article/view/78017 |
Resumo: | Radiological iodinated contrasts (IC) agents cause acute kidney injury (AKI). To evaluate the renoprotective effect of sodium bicarbonate (Bic) on renal function (creatinine clearance {Clcr}, Jaffé, and Clcr mL·min-1⋅100 g-1) and the oxidative profile (peroxide excretion, urinary peroxides, urinary malondialdehyde, FOX-2 expression, and thiobarbituric acid reactive substance {TBARS; nmol/mg Cr}) in rats treated with an IC agent. Adult male Wistar rats weighing 250 - 300 g were treated once daily for 5 days with one of the following treatments: saline (0.9%, 3 mL·kg-1⋅day-1intraperitoneally {i.p.}), IC agent (sodium and meglumine ioxitalamate, 3 mL/kg, i.p.), Bic + Saline (3-mL/kg Bic, i.p., 1 h before and after saline treatment), and Bic + IC (3-mL/kg Bic, i.p., 1 h before and after the IC treatment). The IC agent induced AKI, and the antioxidant renoprotective effect of Bic was confirmed (Clcr/TBARS/urinary peroxide: saline group, 0.59 ± 0.03/0.11 ± 0.02/1.29 ± 0.24; Bic + Saline group, 0.58 ± 0.03/0.13 ± 0.02/1.32 ± 0.64; IC group, 0.22 ± 0.02/0.19 ± 0.02/4.77 ± 0.24; Bic + CI group, 0.51 ± 0.04/0.13 ± 0.3/1.80 ± 0.04; p |
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Revista da Escola de Enfermagem da USP (Online) |
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Protective effect of sodium bicarbonate on radiological contrast medium-induced nephropathy in ratsEfecto protector del bicarbonato de sodio en nefropatia inducida por contraste radiologico en ratonesO efeito protetor do bicarbonato de sodio na nefropatia induzida por contraste radiologico em ratosRadiological iodinated contrasts (IC) agents cause acute kidney injury (AKI). To evaluate the renoprotective effect of sodium bicarbonate (Bic) on renal function (creatinine clearance {Clcr}, Jaffé, and Clcr mL·min-1⋅100 g-1) and the oxidative profile (peroxide excretion, urinary peroxides, urinary malondialdehyde, FOX-2 expression, and thiobarbituric acid reactive substance {TBARS; nmol/mg Cr}) in rats treated with an IC agent. Adult male Wistar rats weighing 250 - 300 g were treated once daily for 5 days with one of the following treatments: saline (0.9%, 3 mL·kg-1⋅day-1intraperitoneally {i.p.}), IC agent (sodium and meglumine ioxitalamate, 3 mL/kg, i.p.), Bic + Saline (3-mL/kg Bic, i.p., 1 h before and after saline treatment), and Bic + IC (3-mL/kg Bic, i.p., 1 h before and after the IC treatment). The IC agent induced AKI, and the antioxidant renoprotective effect of Bic was confirmed (Clcr/TBARS/urinary peroxide: saline group, 0.59 ± 0.03/0.11 ± 0.02/1.29 ± 0.24; Bic + Saline group, 0.58 ± 0.03/0.13 ± 0.02/1.32 ± 0.64; IC group, 0.22 ± 0.02/0.19 ± 0.02/4.77 ± 0.24; Bic + CI group, 0.51 ± 0.04/0.13 ± 0.3/1.80 ± 0.04; pContrastes radiológicos iodados - CI son causa de lesión renal aguda - LRA. Evaluar el efecto renoprotector del bicarbonato de sodio (Bic) en la función renal (clearance de creatinina, Jaffé, Clcr-ml/min/100g) y el perfil oxidativo (excreción de peróxidos, PU y de malondealdehido urinarios, FOX-2 e TBARs, nmol/mgCr) en ratones con CI. Ratones machos adultos Wistar, 250-300g, tratados 1x/día durante 5 días, fueron divididos en grupos: Salina (solución salina 0,9%, 3ml/kg/día, intraperitoneal-i.p.); CI (ioxitalamato de meglumina y sodio, 3ml/kg, i.p); Bic+Salina (Bic 3ml/kg, i.p, 1 hora antes y 1 hora después de la Salina); Bic+CI (Bic 3ml/kg, i.p, 1 hora antes y 1 hora después del CI). CI indujo LRA y el Bic confirmó su efecto renoprotector antioxidante (Clcr/TBARs/PU Salina: 0,59±0,03/0,11±0,02/1,29±0,24 vs Bic+Salina 0,58±0,03/0,13±0,02/1,32±0,64 vs CI 0,22±0,02A/0,19±0,02A/ 4,77±0,24A vs Bic+CI 0,51±0,04B /0,13±0,3B/1,80±0,04B,A/B pContrastes radiológicos iodados - CI são causa de lesão renal aguda - LRA. Avaliar o efeito renoprotetor do bicarbonato de sódio (Bic) sobre a função renal (clearance de creatinina, Jaffé, Clcr-ml/min/100g) e o perfil oxidativo (excreção de peróxidos, PU e de malondealdeído urinários, FOX-2 e TBARs, nmol/mgCr ) em ratos com CI. Ratos machos adultos Wistar, 250-300g, tratados 1x/dia, por 5 dias, foram divididos nos grupos: Salina (solução salina 0,9%, 3ml/kg/dia, intraperitoneal-i.p.); CI (ioxitalamato de meglumina e sódio, 3ml/kg, i.p); Bic+Salina (Bic 3ml/kg, i.p, 1 hora antes e 1 hora depois da Salina); Bic+CI (Bic 3ml/kg, i.p, 1 hora antes e 1 hora depois do CI). CI induziu LRA e o Bic confirmou seu efeito renoprotetor antioxidante (Clcr/TBARs/PU Salina: 0,59±0,03/0,11±0,02/1,29±0,24 vs Bic+Salina 0,58±0,03/0,13±0,02/1,32±0,64 vs CI 0,22±0,02A/0,19±0,02A/4,77±0, 24A vs Bic+CI 0,51±0,04B/0,13±0,3B/1,80± 0,04B, A/B pUniversidade de São Paulo. Escola de Enfermagem2013-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttps://www.revistas.usp.br/reeusp/article/view/7801710.1590/reeusp.v47i3.78017Revista da Escola de Enfermagem da USP; v. 47 n. 3 (2013); 722-727Revista da Escola de Enfermagem da USP; Vol. 47 No. 3 (2013); 722-727Revista da Escola de Enfermagem da USP; Vol. 47 Núm. 3 (2013); 722-7271980-220X0080-6234reponame:Revista da Escola de Enfermagem da USP (Online)instname:Universidade de São Paulo (USP)instacron:USPporenghttps://www.revistas.usp.br/reeusp/article/view/78017/82010https://www.revistas.usp.br/reeusp/article/view/78017/82011http://creativecommons.org/licenses/by-nc/4.0info:eu-repo/semantics/openAccessVattimo, Maria de Fatima FernandesSantos, Juliana Guareschi dos2014-04-01T17:48:08Zoai:revistas.usp.br:article/78017Revistahttps://www.revistas.usp.br/reeuspPUBhttps://www.revistas.usp.br/reeusp/oai||nursingscholar@usp.br1980-220X0080-6234opendoar:2014-04-01T17:48:08Revista da Escola de Enfermagem da USP (Online) - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Protective effect of sodium bicarbonate on radiological contrast medium-induced nephropathy in rats Efecto protector del bicarbonato de sodio en nefropatia inducida por contraste radiologico en ratones O efeito protetor do bicarbonato de sodio na nefropatia induzida por contraste radiologico em ratos |
title |
Protective effect of sodium bicarbonate on radiological contrast medium-induced nephropathy in rats |
spellingShingle |
Protective effect of sodium bicarbonate on radiological contrast medium-induced nephropathy in rats Vattimo, Maria de Fatima Fernandes |
title_short |
Protective effect of sodium bicarbonate on radiological contrast medium-induced nephropathy in rats |
title_full |
Protective effect of sodium bicarbonate on radiological contrast medium-induced nephropathy in rats |
title_fullStr |
Protective effect of sodium bicarbonate on radiological contrast medium-induced nephropathy in rats |
title_full_unstemmed |
Protective effect of sodium bicarbonate on radiological contrast medium-induced nephropathy in rats |
title_sort |
Protective effect of sodium bicarbonate on radiological contrast medium-induced nephropathy in rats |
author |
Vattimo, Maria de Fatima Fernandes |
author_facet |
Vattimo, Maria de Fatima Fernandes Santos, Juliana Guareschi dos |
author_role |
author |
author2 |
Santos, Juliana Guareschi dos |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Vattimo, Maria de Fatima Fernandes Santos, Juliana Guareschi dos |
description |
Radiological iodinated contrasts (IC) agents cause acute kidney injury (AKI). To evaluate the renoprotective effect of sodium bicarbonate (Bic) on renal function (creatinine clearance {Clcr}, Jaffé, and Clcr mL·min-1⋅100 g-1) and the oxidative profile (peroxide excretion, urinary peroxides, urinary malondialdehyde, FOX-2 expression, and thiobarbituric acid reactive substance {TBARS; nmol/mg Cr}) in rats treated with an IC agent. Adult male Wistar rats weighing 250 - 300 g were treated once daily for 5 days with one of the following treatments: saline (0.9%, 3 mL·kg-1⋅day-1intraperitoneally {i.p.}), IC agent (sodium and meglumine ioxitalamate, 3 mL/kg, i.p.), Bic + Saline (3-mL/kg Bic, i.p., 1 h before and after saline treatment), and Bic + IC (3-mL/kg Bic, i.p., 1 h before and after the IC treatment). The IC agent induced AKI, and the antioxidant renoprotective effect of Bic was confirmed (Clcr/TBARS/urinary peroxide: saline group, 0.59 ± 0.03/0.11 ± 0.02/1.29 ± 0.24; Bic + Saline group, 0.58 ± 0.03/0.13 ± 0.02/1.32 ± 0.64; IC group, 0.22 ± 0.02/0.19 ± 0.02/4.77 ± 0.24; Bic + CI group, 0.51 ± 0.04/0.13 ± 0.3/1.80 ± 0.04; p |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/reeusp/article/view/78017 10.1590/reeusp.v47i3.78017 |
url |
https://www.revistas.usp.br/reeusp/article/view/78017 |
identifier_str_mv |
10.1590/reeusp.v47i3.78017 |
dc.language.iso.fl_str_mv |
por eng |
language |
por eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/reeusp/article/view/78017/82010 https://www.revistas.usp.br/reeusp/article/view/78017/82011 |
dc.rights.driver.fl_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
http://creativecommons.org/licenses/by-nc/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Escola de Enfermagem |
publisher.none.fl_str_mv |
Universidade de São Paulo. Escola de Enfermagem |
dc.source.none.fl_str_mv |
Revista da Escola de Enfermagem da USP; v. 47 n. 3 (2013); 722-727 Revista da Escola de Enfermagem da USP; Vol. 47 No. 3 (2013); 722-727 Revista da Escola de Enfermagem da USP; Vol. 47 Núm. 3 (2013); 722-727 1980-220X 0080-6234 reponame:Revista da Escola de Enfermagem da USP (Online) instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Revista da Escola de Enfermagem da USP (Online) |
collection |
Revista da Escola de Enfermagem da USP (Online) |
repository.name.fl_str_mv |
Revista da Escola de Enfermagem da USP (Online) - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
||nursingscholar@usp.br |
_version_ |
1800221736314077184 |