Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice

Detalhes bibliográficos
Autor(a) principal: Neiva,Luciana Barros de Moura
Data de Publicação: 2014
Outros Autores: Borges,Fernanda Teixeira, Watanabe,Mirian, Pessoa,Edson de Andrade, Barbosa,Dulce Aparecida, Vattimo,Maria de Fatima Fernandes
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Revista da Escola de Enfermagem da USP (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0080-62342014000200272
Resumo: The aim of the study was to characterize the cell damage mechanisms involved in the pathophysiology of cytotoxicity of polymyxin B in proximal tubular cells (LLC - PK1) and discuss about the nurses interventions to identify at risk patients and consider prevention or treatment of nephrotoxicity acute kidney injury. This is a quantitative experimental in vitro study, in which the cells were exposed to 375μM polymyxin B sulfate concentration. Cell viability was determined by exclusion of fluorescent dyes and morphological method with visualization of apoptotic bodies for fluorescence microscopy. Cells exposed to polymyxin B showed reduced viability, increased number of apoptotic cells and a higher concentration of the enzyme lactate dehydrogenase. The administration of polymyxin B in vitro showed the need for actions to minimize adverse effects such as nephrotoxicity.

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spelling Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing PracticePolymyxin BAnti-bacterial agentsAcute kidney injuryLLC-PK1 cellsNursing careThe aim of the study was to characterize the cell damage mechanisms involved in the pathophysiology of cytotoxicity of polymyxin B in proximal tubular cells (LLC - PK1) and discuss about the nurses interventions to identify at risk patients and consider prevention or treatment of nephrotoxicity acute kidney injury. This is a quantitative experimental in vitro study, in which the cells were exposed to 375μM polymyxin B sulfate concentration. Cell viability was determined by exclusion of fluorescent dyes and morphological method with visualization of apoptotic bodies for fluorescence microscopy. Cells exposed to polymyxin B showed reduced viability, increased number of apoptotic cells and a higher concentration of the enzyme lactate dehydrogenase. The administration of polymyxin B in vitro showed the need for actions to minimize adverse effects such as nephrotoxicity.
Universidade de São Paulo, Escola de Enfermagem2014-04-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0080-62342014000200272Revista da Escola de Enfermagem da USP v.48 n.2 2014reponame:Revista da Escola de Enfermagem da USP (Online)instname:Universidade de São Paulo (USP)instacron:USP10.1590/S0080-6234201400002000011info:eu-repo/semantics/openAccessNeiva,Luciana Barros de MouraBorges,Fernanda TeixeiraWatanabe,MirianPessoa,Edson de AndradeBarbosa,Dulce AparecidaVattimo,Maria de Fatima Fernandeseng2014-10-14T00:00:00Zoai:scielo:S0080-62342014000200272Revistahttp://www.scielo.br/reeuspPUBhttps://old.scielo.br/oai/scielo-oai.php||nursingscholar@usp.br1980-220X0080-6234opendoar:2014-10-14T00:00Revista da Escola de Enfermagem da USP (Online) - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice
title Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice
spellingShingle Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice
Neiva,Luciana Barros de Moura
Polymyxin B
Anti-bacterial agents
Acute kidney injury
LLC-PK1 cells
Nursing care
title_short Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice
title_full Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice
title_fullStr Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice
title_full_unstemmed Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice
title_sort Nephrotoxicity Of Polymyxin B: Experimental Study In Cells And Implications For Nursing Practice
author Neiva,Luciana Barros de Moura
author_facet Neiva,Luciana Barros de Moura
Borges,Fernanda Teixeira
Watanabe,Mirian
Pessoa,Edson de Andrade
Barbosa,Dulce Aparecida
Vattimo,Maria de Fatima Fernandes
author_role author
author2 Borges,Fernanda Teixeira
Watanabe,Mirian
Pessoa,Edson de Andrade
Barbosa,Dulce Aparecida
Vattimo,Maria de Fatima Fernandes
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Neiva,Luciana Barros de Moura
Borges,Fernanda Teixeira
Watanabe,Mirian
Pessoa,Edson de Andrade
Barbosa,Dulce Aparecida
Vattimo,Maria de Fatima Fernandes
dc.subject.por.fl_str_mv Polymyxin B
Anti-bacterial agents
Acute kidney injury
LLC-PK1 cells
Nursing care
topic Polymyxin B
Anti-bacterial agents
Acute kidney injury
LLC-PK1 cells
Nursing care
description The aim of the study was to characterize the cell damage mechanisms involved in the pathophysiology of cytotoxicity of polymyxin B in proximal tubular cells (LLC - PK1) and discuss about the nurses interventions to identify at risk patients and consider prevention or treatment of nephrotoxicity acute kidney injury. This is a quantitative experimental in vitro study, in which the cells were exposed to 375μM polymyxin B sulfate concentration. Cell viability was determined by exclusion of fluorescent dyes and morphological method with visualization of apoptotic bodies for fluorescence microscopy. Cells exposed to polymyxin B showed reduced viability, increased number of apoptotic cells and a higher concentration of the enzyme lactate dehydrogenase. The administration of polymyxin B in vitro showed the need for actions to minimize adverse effects such as nephrotoxicity.

publishDate 2014
dc.date.none.fl_str_mv 2014-04-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0080-62342014000200272
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0080-62342014000200272
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S0080-6234201400002000011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Universidade de São Paulo, Escola de Enfermagem
publisher.none.fl_str_mv Universidade de São Paulo, Escola de Enfermagem
dc.source.none.fl_str_mv Revista da Escola de Enfermagem da USP v.48 n.2 2014
reponame:Revista da Escola de Enfermagem da USP (Online)
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Revista da Escola de Enfermagem da USP (Online)
collection Revista da Escola de Enfermagem da USP (Online)
repository.name.fl_str_mv Revista da Escola de Enfermagem da USP (Online) - Universidade de São Paulo (USP)
repository.mail.fl_str_mv ||nursingscholar@usp.br
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