In vitro cytotoxicity of chemical preservatives on human fibroblast cells
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/146819 |
Resumo: | Preservatives are widely used substances that are commonly added to various cosmetic and pharmaceutical products to prevent or inhibit microbial growth. In this study, we compared the in vitro cytotoxicity of different types of currently used preservatives, including methylparaben, imidazolidinyl urea (IMU), and sodium benzoate, using the human newborn fibroblast cell line CCD1072Sk. Of the tested preservatives, only IMU induced a reduction in cell viability, as shown using the MTT assay and propidium iodide staining (IMU>;methylparaben>;sodium benzoate). IMU was shown to promote homeostatic alterations potentially related to the initiation of programed cell death, such as decreased mitochondrial membrane potential and caspase-3 activation, in the treated cells. Methylparaben and sodium benzoate were shown to have a very low cytotoxic activity. Taken together, our results suggest that IMU induces programed cell death in human fibroblasts by a canonical intrinsic pathway via mitochondrial perturbation and subsequent release of proapoptotic factors. |
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Brazilian Journal of Pharmaceutical Sciences |
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In vitro cytotoxicity of chemical preservatives on human fibroblast cellsPreservativesPharmaceutical/chemistryFibroblasts/cytotoxicityCell death/drug effectsCosmetics/additives Preservatives are widely used substances that are commonly added to various cosmetic and pharmaceutical products to prevent or inhibit microbial growth. In this study, we compared the in vitro cytotoxicity of different types of currently used preservatives, including methylparaben, imidazolidinyl urea (IMU), and sodium benzoate, using the human newborn fibroblast cell line CCD1072Sk. Of the tested preservatives, only IMU induced a reduction in cell viability, as shown using the MTT assay and propidium iodide staining (IMU>;methylparaben>;sodium benzoate). IMU was shown to promote homeostatic alterations potentially related to the initiation of programed cell death, such as decreased mitochondrial membrane potential and caspase-3 activation, in the treated cells. Methylparaben and sodium benzoate were shown to have a very low cytotoxic activity. Taken together, our results suggest that IMU induces programed cell death in human fibroblasts by a canonical intrinsic pathway via mitochondrial perturbation and subsequent release of proapoptotic factors.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2018-06-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/14681910.1590/s2175-97902018000100031Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 1 (2018); e00031Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 1 (2018); e00031Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 1 (2018); e000312175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/146819/140348Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessSpindola, Daniel GonsalesHinsberger, AndreAntunes, Valéria Maria de SouzaMichelin, Luis Felipe GomesBincoletto, ClaudiaOliveira, Carlos Rocha2018-06-07T16:31:56Zoai:revistas.usp.br:article/146819Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2018-06-07T16:31:56Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
In vitro cytotoxicity of chemical preservatives on human fibroblast cells |
title |
In vitro cytotoxicity of chemical preservatives on human fibroblast cells |
spellingShingle |
In vitro cytotoxicity of chemical preservatives on human fibroblast cells Spindola, Daniel Gonsales Preservatives Pharmaceutical/chemistry Fibroblasts/cytotoxicity Cell death/drug effects Cosmetics/additives |
title_short |
In vitro cytotoxicity of chemical preservatives on human fibroblast cells |
title_full |
In vitro cytotoxicity of chemical preservatives on human fibroblast cells |
title_fullStr |
In vitro cytotoxicity of chemical preservatives on human fibroblast cells |
title_full_unstemmed |
In vitro cytotoxicity of chemical preservatives on human fibroblast cells |
title_sort |
In vitro cytotoxicity of chemical preservatives on human fibroblast cells |
author |
Spindola, Daniel Gonsales |
author_facet |
Spindola, Daniel Gonsales Hinsberger, Andre Antunes, Valéria Maria de Souza Michelin, Luis Felipe Gomes Bincoletto, Claudia Oliveira, Carlos Rocha |
author_role |
author |
author2 |
Hinsberger, Andre Antunes, Valéria Maria de Souza Michelin, Luis Felipe Gomes Bincoletto, Claudia Oliveira, Carlos Rocha |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Spindola, Daniel Gonsales Hinsberger, Andre Antunes, Valéria Maria de Souza Michelin, Luis Felipe Gomes Bincoletto, Claudia Oliveira, Carlos Rocha |
dc.subject.por.fl_str_mv |
Preservatives Pharmaceutical/chemistry Fibroblasts/cytotoxicity Cell death/drug effects Cosmetics/additives |
topic |
Preservatives Pharmaceutical/chemistry Fibroblasts/cytotoxicity Cell death/drug effects Cosmetics/additives |
description |
Preservatives are widely used substances that are commonly added to various cosmetic and pharmaceutical products to prevent or inhibit microbial growth. In this study, we compared the in vitro cytotoxicity of different types of currently used preservatives, including methylparaben, imidazolidinyl urea (IMU), and sodium benzoate, using the human newborn fibroblast cell line CCD1072Sk. Of the tested preservatives, only IMU induced a reduction in cell viability, as shown using the MTT assay and propidium iodide staining (IMU>;methylparaben>;sodium benzoate). IMU was shown to promote homeostatic alterations potentially related to the initiation of programed cell death, such as decreased mitochondrial membrane potential and caspase-3 activation, in the treated cells. Methylparaben and sodium benzoate were shown to have a very low cytotoxic activity. Taken together, our results suggest that IMU induces programed cell death in human fibroblasts by a canonical intrinsic pathway via mitochondrial perturbation and subsequent release of proapoptotic factors. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/146819 10.1590/s2175-97902018000100031 |
url |
https://www.revistas.usp.br/bjps/article/view/146819 |
identifier_str_mv |
10.1590/s2175-97902018000100031 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/146819/140348 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 1 (2018); e00031 Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 1 (2018); e00031 Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 1 (2018); e00031 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222913350074368 |