99m Technetium-HMPAO-labeled platelet scan in practice: Preparation, quality control, and biodistribution studies
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/204483 |
Resumo: | There is no biodistribution or imaging data on 99mtechnetium (Tc)-hexamethyl propylamine oxime (HMPAO)-labeled platelets in the literature. The current study aimed to present updated information about the clinical procedures for preparation and use of labeled platelets. Following two-step centrifugation at 1500 and 2500 rpm, the platelets were extracted from whole blood into platelet-rich plasma (PRP) above the buffy coat and then from PRP into a platelet pellet at the bottom of the tube. The 99mTc-HMPAO-labeled platelets were inspected for purity, viability, release of 99mTc from platelets, and sterility. Also, microscopic examination and thin layer chromatography (TLC) were performed. Biodistribution was assessed following necropsy in BALB/c mice and through imaging of New Zealand rabbits. The separation ratio was estimated at 98%, and radiochemical purity was measured to be 80%. The labeling efficiency was above 30% in more than half of the assays (range: 17-43%). The release of 99mTc from platelets was 9% per hour at 37ºC. After 24 hours, stability was estimated at 54% in the human serum. The target organs of mice included the spleen and liver. In rabbits, the imaging results indicated liver as the target organ. Thyroid uptake was negligible up to 90 minutes. Based on the findings, extraction of platelets and labeling them with 99mTc-HMPAO is a feasible and safe approach in routine practice. |
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Brazilian Journal of Pharmaceutical Sciences |
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99m Technetium-HMPAO-labeled platelet scan in practice: Preparation, quality control, and biodistribution studiesPlatelet; 99mTc-HMPAO; Biodistribution; Labeling efficiency; Platelet extractionThere is no biodistribution or imaging data on 99mtechnetium (Tc)-hexamethyl propylamine oxime (HMPAO)-labeled platelets in the literature. The current study aimed to present updated information about the clinical procedures for preparation and use of labeled platelets. Following two-step centrifugation at 1500 and 2500 rpm, the platelets were extracted from whole blood into platelet-rich plasma (PRP) above the buffy coat and then from PRP into a platelet pellet at the bottom of the tube. The 99mTc-HMPAO-labeled platelets were inspected for purity, viability, release of 99mTc from platelets, and sterility. Also, microscopic examination and thin layer chromatography (TLC) were performed. Biodistribution was assessed following necropsy in BALB/c mice and through imaging of New Zealand rabbits. The separation ratio was estimated at 98%, and radiochemical purity was measured to be 80%. The labeling efficiency was above 30% in more than half of the assays (range: 17-43%). The release of 99mTc from platelets was 9% per hour at 37ºC. After 24 hours, stability was estimated at 54% in the human serum. The target organs of mice included the spleen and liver. In rabbits, the imaging results indicated liver as the target organ. Thyroid uptake was negligible up to 90 minutes. Based on the findings, extraction of platelets and labeling them with 99mTc-HMPAO is a feasible and safe approach in routine practice.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2022-11-17info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/20448310.1590/s2175-97902022e18860Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/204483/194944Copyright (c) 2022 Brazilian Journal of Pharmaceutical Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessParvizi, MahdiehFarzanefar, SaeedKhalaj, AliTafakhori, AbbasJohari Daha, FaribaSaffar, HanaSadeghzadeh, MasoudNaseri, MaryamAhmadzadehfar, Hojjat Abbasi, Mehrshad2023-08-18T20:45:05Zoai:revistas.usp.br:article/204483Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2023-08-18T20:45:05Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
99m Technetium-HMPAO-labeled platelet scan in practice: Preparation, quality control, and biodistribution studies |
title |
99m Technetium-HMPAO-labeled platelet scan in practice: Preparation, quality control, and biodistribution studies |
spellingShingle |
99m Technetium-HMPAO-labeled platelet scan in practice: Preparation, quality control, and biodistribution studies Parvizi, Mahdieh Platelet; 99mTc-HMPAO; Biodistribution; Labeling efficiency; Platelet extraction |
title_short |
99m Technetium-HMPAO-labeled platelet scan in practice: Preparation, quality control, and biodistribution studies |
title_full |
99m Technetium-HMPAO-labeled platelet scan in practice: Preparation, quality control, and biodistribution studies |
title_fullStr |
99m Technetium-HMPAO-labeled platelet scan in practice: Preparation, quality control, and biodistribution studies |
title_full_unstemmed |
99m Technetium-HMPAO-labeled platelet scan in practice: Preparation, quality control, and biodistribution studies |
title_sort |
99m Technetium-HMPAO-labeled platelet scan in practice: Preparation, quality control, and biodistribution studies |
author |
Parvizi, Mahdieh |
author_facet |
Parvizi, Mahdieh Farzanefar, Saeed Khalaj, Ali Tafakhori, Abbas Johari Daha, Fariba Saffar, Hana Sadeghzadeh, Masoud Naseri, Maryam Ahmadzadehfar, Hojjat Abbasi, Mehrshad |
author_role |
author |
author2 |
Farzanefar, Saeed Khalaj, Ali Tafakhori, Abbas Johari Daha, Fariba Saffar, Hana Sadeghzadeh, Masoud Naseri, Maryam Ahmadzadehfar, Hojjat Abbasi, Mehrshad |
author2_role |
author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Parvizi, Mahdieh Farzanefar, Saeed Khalaj, Ali Tafakhori, Abbas Johari Daha, Fariba Saffar, Hana Sadeghzadeh, Masoud Naseri, Maryam Ahmadzadehfar, Hojjat Abbasi, Mehrshad |
dc.subject.por.fl_str_mv |
Platelet; 99mTc-HMPAO; Biodistribution; Labeling efficiency; Platelet extraction |
topic |
Platelet; 99mTc-HMPAO; Biodistribution; Labeling efficiency; Platelet extraction |
description |
There is no biodistribution or imaging data on 99mtechnetium (Tc)-hexamethyl propylamine oxime (HMPAO)-labeled platelets in the literature. The current study aimed to present updated information about the clinical procedures for preparation and use of labeled platelets. Following two-step centrifugation at 1500 and 2500 rpm, the platelets were extracted from whole blood into platelet-rich plasma (PRP) above the buffy coat and then from PRP into a platelet pellet at the bottom of the tube. The 99mTc-HMPAO-labeled platelets were inspected for purity, viability, release of 99mTc from platelets, and sterility. Also, microscopic examination and thin layer chromatography (TLC) were performed. Biodistribution was assessed following necropsy in BALB/c mice and through imaging of New Zealand rabbits. The separation ratio was estimated at 98%, and radiochemical purity was measured to be 80%. The labeling efficiency was above 30% in more than half of the assays (range: 17-43%). The release of 99mTc from platelets was 9% per hour at 37ºC. After 24 hours, stability was estimated at 54% in the human serum. The target organs of mice included the spleen and liver. In rabbits, the imaging results indicated liver as the target organ. Thyroid uptake was negligible up to 90 minutes. Based on the findings, extraction of platelets and labeling them with 99mTc-HMPAO is a feasible and safe approach in routine practice. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-17 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/204483 10.1590/s2175-97902022e18860 |
url |
https://www.revistas.usp.br/bjps/article/view/204483 |
identifier_str_mv |
10.1590/s2175-97902022e18860 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/204483/194944 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022) Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022) Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022) 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222916065886208 |