Vitamin C as a shelf-life extender in liposomes

Detalhes bibliográficos
Autor(a) principal: Reis Favarin, Fernanda
Data de Publicação: 2023
Outros Autores: da Silva Gündel, Samanta, Mafra Ledur, Cristian, Roggia, Isabel, Binotto Fagan, Solange, Gündel, André, de Oliveira Fogaça, Aline, Ferreira Ourique, Aline
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Pharmaceutical Sciences
Texto Completo: https://www.revistas.usp.br/bjps/article/view/207257
Resumo: The objective of this study was to evaluate the influence of vitamin C (VC) on the stability of stored liposomes under different climatic conditions. Liposomal formulations containing 1 mg/mL of VC (LIP-VC) and blank formulations (LIP-B) were prepared by the reverse-phase evaporation method. After preparation, they were characterized according to their refractive index, average vesicle diameter, polydispersity index (PDI), zeta potential, pH, content, encapsulation efficiency (EE%), morphology, stability and antioxidant activity. For stability, LIP-VC and LIP-B were stored in different climatic conditions (4 °C, 25 °C and 40 °C) for 30 days. The LIP-VC presented 1.3365 refractive index, 161 nm of mean diameter, 0.231 PDI, -7.3 mV zeta potential, 3.2 pH, 19.4% EE%, spherical morphology, 1 mg/mL of VC content, and antioxidant activity of 12 and 11.4 μmol of TE/mL for the radical DPPH and ABTS+, respectively. During stability, the LIP-B stored in 40 °C showed an instability in the parameters: PDI, vesicle size and zeta potential after 15 days, while the LIP-VC remained stable in its size and PDI for 30 days. After that, it is shown that VC can be used as an antioxidant and stabilizer in liposomes to increase the stability and shelf-life of vesicles.
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spelling Vitamin C as a shelf-life extender in liposomesAscorbic acidAntioxidantNanoliposomesAntioxidant activityReverse-phase evaporationThe objective of this study was to evaluate the influence of vitamin C (VC) on the stability of stored liposomes under different climatic conditions. Liposomal formulations containing 1 mg/mL of VC (LIP-VC) and blank formulations (LIP-B) were prepared by the reverse-phase evaporation method. After preparation, they were characterized according to their refractive index, average vesicle diameter, polydispersity index (PDI), zeta potential, pH, content, encapsulation efficiency (EE%), morphology, stability and antioxidant activity. For stability, LIP-VC and LIP-B were stored in different climatic conditions (4 °C, 25 °C and 40 °C) for 30 days. The LIP-VC presented 1.3365 refractive index, 161 nm of mean diameter, 0.231 PDI, -7.3 mV zeta potential, 3.2 pH, 19.4% EE%, spherical morphology, 1 mg/mL of VC content, and antioxidant activity of 12 and 11.4 μmol of TE/mL for the radical DPPH and ABTS+, respectively. During stability, the LIP-B stored in 40 °C showed an instability in the parameters: PDI, vesicle size and zeta potential after 15 days, while the LIP-VC remained stable in its size and PDI for 30 days. After that, it is shown that VC can be used as an antioxidant and stabilizer in liposomes to increase the stability and shelf-life of vesicles.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2023-01-26info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/20725710.1590/s2175-97902022e20492Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/207257/197610Copyright (c) 2022 Brazilian Journal of Pharmaceutical Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessReis Favarin, Fernandada Silva Gündel, SamantaMafra Ledur, CristianRoggia, IsabelBinotto Fagan, SolangeGündel, Andréde Oliveira Fogaça, AlineFerreira Ourique, Aline2023-08-30T15:55:53Zoai:revistas.usp.br:article/207257Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2023-08-30T15:55:53Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Vitamin C as a shelf-life extender in liposomes
title Vitamin C as a shelf-life extender in liposomes
spellingShingle Vitamin C as a shelf-life extender in liposomes
Reis Favarin, Fernanda
Ascorbic acid
Antioxidant
Nanoliposomes
Antioxidant activity
Reverse-phase evaporation
title_short Vitamin C as a shelf-life extender in liposomes
title_full Vitamin C as a shelf-life extender in liposomes
title_fullStr Vitamin C as a shelf-life extender in liposomes
title_full_unstemmed Vitamin C as a shelf-life extender in liposomes
title_sort Vitamin C as a shelf-life extender in liposomes
author Reis Favarin, Fernanda
author_facet Reis Favarin, Fernanda
da Silva Gündel, Samanta
Mafra Ledur, Cristian
Roggia, Isabel
Binotto Fagan, Solange
Gündel, André
de Oliveira Fogaça, Aline
Ferreira Ourique, Aline
author_role author
author2 da Silva Gündel, Samanta
Mafra Ledur, Cristian
Roggia, Isabel
Binotto Fagan, Solange
Gündel, André
de Oliveira Fogaça, Aline
Ferreira Ourique, Aline
author2_role author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Reis Favarin, Fernanda
da Silva Gündel, Samanta
Mafra Ledur, Cristian
Roggia, Isabel
Binotto Fagan, Solange
Gündel, André
de Oliveira Fogaça, Aline
Ferreira Ourique, Aline
dc.subject.por.fl_str_mv Ascorbic acid
Antioxidant
Nanoliposomes
Antioxidant activity
Reverse-phase evaporation
topic Ascorbic acid
Antioxidant
Nanoliposomes
Antioxidant activity
Reverse-phase evaporation
description The objective of this study was to evaluate the influence of vitamin C (VC) on the stability of stored liposomes under different climatic conditions. Liposomal formulations containing 1 mg/mL of VC (LIP-VC) and blank formulations (LIP-B) were prepared by the reverse-phase evaporation method. After preparation, they were characterized according to their refractive index, average vesicle diameter, polydispersity index (PDI), zeta potential, pH, content, encapsulation efficiency (EE%), morphology, stability and antioxidant activity. For stability, LIP-VC and LIP-B were stored in different climatic conditions (4 °C, 25 °C and 40 °C) for 30 days. The LIP-VC presented 1.3365 refractive index, 161 nm of mean diameter, 0.231 PDI, -7.3 mV zeta potential, 3.2 pH, 19.4% EE%, spherical morphology, 1 mg/mL of VC content, and antioxidant activity of 12 and 11.4 μmol of TE/mL for the radical DPPH and ABTS+, respectively. During stability, the LIP-B stored in 40 °C showed an instability in the parameters: PDI, vesicle size and zeta potential after 15 days, while the LIP-VC remained stable in its size and PDI for 30 days. After that, it is shown that VC can be used as an antioxidant and stabilizer in liposomes to increase the stability and shelf-life of vesicles.
publishDate 2023
dc.date.none.fl_str_mv 2023-01-26
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/207257
10.1590/s2175-97902022e20492
url https://www.revistas.usp.br/bjps/article/view/207257
identifier_str_mv 10.1590/s2175-97902022e20492
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/207257/197610
dc.rights.driver.fl_str_mv Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)
Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022)
Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
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