Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/131427 |
Resumo: | Peptic ulcers are an important pathology, and the search for safer and more effective treatment methods is of paramount importance. In this study, we assess the gastroprotective effects of the hydroethanolic extract (HE) and ethyl acetate fraction (EAF) from Kalanchoe pinnata leaves against an ethanol/HCl-induced ulcer model in rats. The HE reduced gastric lesions by approximately 47% (400 mg/kg). A significant inhibition of the gastric lesions by 50% was observed after pretreatment with the EAF (200 mg/kg). Quercetrin and quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside were isolated and identified in the flavonoid fraction (EAF) by HPLC and NMR analyses because this fraction showed the highest gastroprotective effect. This fraction demonstrated high antioxidant activities (CE50=41.91 µg/mL) by DPPH in comparison with Trolox(r) and 11.33 mmol Trolox(r) equivalent by ORAC. In conclusion, the HE and FAE from K. pinnata displayed gastroprotective activity in rats, most likely due to the presence of flavonoids. |
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Brazilian Journal of Pharmaceutical Sciences |
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Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.Gastric ulcer/study/ratsGastric ulcer/treatmentKalanchoe pinnata/chromatographic profileKalanchoe pinnata/gastroprotective activityQuercetrinQuercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside.Peptic ulcers are an important pathology, and the search for safer and more effective treatment methods is of paramount importance. In this study, we assess the gastroprotective effects of the hydroethanolic extract (HE) and ethyl acetate fraction (EAF) from Kalanchoe pinnata leaves against an ethanol/HCl-induced ulcer model in rats. The HE reduced gastric lesions by approximately 47% (400 mg/kg). A significant inhibition of the gastric lesions by 50% was observed after pretreatment with the EAF (200 mg/kg). Quercetrin and quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside were isolated and identified in the flavonoid fraction (EAF) by HPLC and NMR analyses because this fraction showed the highest gastroprotective effect. This fraction demonstrated high antioxidant activities (CE50=41.91 µg/mL) by DPPH in comparison with Trolox(r) and 11.33 mmol Trolox(r) equivalent by ORAC. In conclusion, the HE and FAE from K. pinnata displayed gastroprotective activity in rats, most likely due to the presence of flavonoids.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/13142710.1590/s2175-97902017000116027Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 1 (2017); e16027-Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 1 (2017); e16027-Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 1 (2017); e16027-2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/131427/127807Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessSobreira, FláviaHernandes, Leandro SantoroVetore-Neto, AlbertoDíaz, Ingrit E. CollantesSantana, Fernanda Carvalho deMancini-Filho, JorgeBacchi, Elfriede Marianne2017-04-20T20:28:50Zoai:revistas.usp.br:article/131427Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2017-04-20T20:28:50Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers. |
title |
Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers. |
spellingShingle |
Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers. Sobreira, Flávia Gastric ulcer/study/rats Gastric ulcer/treatment Kalanchoe pinnata/chromatographic profile Kalanchoe pinnata/gastroprotective activity Quercetrin Quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside. |
title_short |
Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers. |
title_full |
Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers. |
title_fullStr |
Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers. |
title_full_unstemmed |
Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers. |
title_sort |
Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers. |
author |
Sobreira, Flávia |
author_facet |
Sobreira, Flávia Hernandes, Leandro Santoro Vetore-Neto, Alberto Díaz, Ingrit E. Collantes Santana, Fernanda Carvalho de Mancini-Filho, Jorge Bacchi, Elfriede Marianne |
author_role |
author |
author2 |
Hernandes, Leandro Santoro Vetore-Neto, Alberto Díaz, Ingrit E. Collantes Santana, Fernanda Carvalho de Mancini-Filho, Jorge Bacchi, Elfriede Marianne |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Sobreira, Flávia Hernandes, Leandro Santoro Vetore-Neto, Alberto Díaz, Ingrit E. Collantes Santana, Fernanda Carvalho de Mancini-Filho, Jorge Bacchi, Elfriede Marianne |
dc.subject.por.fl_str_mv |
Gastric ulcer/study/rats Gastric ulcer/treatment Kalanchoe pinnata/chromatographic profile Kalanchoe pinnata/gastroprotective activity Quercetrin Quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside. |
topic |
Gastric ulcer/study/rats Gastric ulcer/treatment Kalanchoe pinnata/chromatographic profile Kalanchoe pinnata/gastroprotective activity Quercetrin Quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside. |
description |
Peptic ulcers are an important pathology, and the search for safer and more effective treatment methods is of paramount importance. In this study, we assess the gastroprotective effects of the hydroethanolic extract (HE) and ethyl acetate fraction (EAF) from Kalanchoe pinnata leaves against an ethanol/HCl-induced ulcer model in rats. The HE reduced gastric lesions by approximately 47% (400 mg/kg). A significant inhibition of the gastric lesions by 50% was observed after pretreatment with the EAF (200 mg/kg). Quercetrin and quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside were isolated and identified in the flavonoid fraction (EAF) by HPLC and NMR analyses because this fraction showed the highest gastroprotective effect. This fraction demonstrated high antioxidant activities (CE50=41.91 µg/mL) by DPPH in comparison with Trolox(r) and 11.33 mmol Trolox(r) equivalent by ORAC. In conclusion, the HE and FAE from K. pinnata displayed gastroprotective activity in rats, most likely due to the presence of flavonoids. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/131427 10.1590/s2175-97902017000116027 |
url |
https://www.revistas.usp.br/bjps/article/view/131427 |
identifier_str_mv |
10.1590/s2175-97902017000116027 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/131427/127807 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 1 (2017); e16027- Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 1 (2017); e16027- Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 1 (2017); e16027- 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222912921206784 |