Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.

Detalhes bibliográficos
Autor(a) principal: Sobreira, Flávia
Data de Publicação: 2017
Outros Autores: Hernandes, Leandro Santoro, Vetore-Neto, Alberto, Díaz, Ingrit E. Collantes, Santana, Fernanda Carvalho de, Mancini-Filho, Jorge, Bacchi, Elfriede Marianne
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Pharmaceutical Sciences
Texto Completo: https://www.revistas.usp.br/bjps/article/view/131427
Resumo: Peptic ulcers are an important pathology, and the search for safer and more effective treatment methods is of paramount importance. In this study, we assess the gastroprotective effects of the hydroethanolic extract (HE) and ethyl acetate fraction (EAF) from Kalanchoe pinnata leaves against an ethanol/HCl-induced ulcer model in rats. The HE reduced gastric lesions by approximately 47% (400 mg/kg). A significant inhibition of the gastric lesions by 50% was observed after pretreatment with the EAF (200 mg/kg). Quercetrin and quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside were isolated and identified in the flavonoid fraction (EAF) by HPLC and NMR analyses because this fraction showed the highest gastroprotective effect. This fraction demonstrated high antioxidant activities (CE50=41.91 µg/mL) by DPPH in comparison with Trolox(r) and 11.33 mmol Trolox(r) equivalent by ORAC. In conclusion, the HE and FAE from K. pinnata displayed gastroprotective activity in rats, most likely due to the presence of flavonoids.
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spelling Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.Gastric ulcer/study/ratsGastric ulcer/treatmentKalanchoe pinnata/chromatographic profileKalanchoe pinnata/gastroprotective activityQuercetrinQuercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside.Peptic ulcers are an important pathology, and the search for safer and more effective treatment methods is of paramount importance. In this study, we assess the gastroprotective effects of the hydroethanolic extract (HE) and ethyl acetate fraction (EAF) from Kalanchoe pinnata leaves against an ethanol/HCl-induced ulcer model in rats. The HE reduced gastric lesions by approximately 47% (400 mg/kg). A significant inhibition of the gastric lesions by 50% was observed after pretreatment with the EAF (200 mg/kg). Quercetrin and quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside were isolated and identified in the flavonoid fraction (EAF) by HPLC and NMR analyses because this fraction showed the highest gastroprotective effect. This fraction demonstrated high antioxidant activities (CE50=41.91 µg/mL) by DPPH in comparison with Trolox(r) and 11.33 mmol Trolox(r) equivalent by ORAC. In conclusion, the HE and FAE from K. pinnata displayed gastroprotective activity in rats, most likely due to the presence of flavonoids.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/13142710.1590/s2175-97902017000116027Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 1 (2017); e16027-Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 1 (2017); e16027-Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 1 (2017); e16027-2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/131427/127807Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessSobreira, FláviaHernandes, Leandro SantoroVetore-Neto, AlbertoDíaz, Ingrit E. CollantesSantana, Fernanda Carvalho deMancini-Filho, JorgeBacchi, Elfriede Marianne2017-04-20T20:28:50Zoai:revistas.usp.br:article/131427Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2017-04-20T20:28:50Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.
title Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.
spellingShingle Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.
Sobreira, Flávia
Gastric ulcer/study/rats
Gastric ulcer/treatment
Kalanchoe pinnata/chromatographic profile
Kalanchoe pinnata/gastroprotective activity
Quercetrin
Quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside.
title_short Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.
title_full Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.
title_fullStr Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.
title_full_unstemmed Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.
title_sort Gastroprotective activity of the hydroethanolic extract and ethyl acetate fraction from Kalanchoe pinnata (Lam.) Pers.
author Sobreira, Flávia
author_facet Sobreira, Flávia
Hernandes, Leandro Santoro
Vetore-Neto, Alberto
Díaz, Ingrit E. Collantes
Santana, Fernanda Carvalho de
Mancini-Filho, Jorge
Bacchi, Elfriede Marianne
author_role author
author2 Hernandes, Leandro Santoro
Vetore-Neto, Alberto
Díaz, Ingrit E. Collantes
Santana, Fernanda Carvalho de
Mancini-Filho, Jorge
Bacchi, Elfriede Marianne
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sobreira, Flávia
Hernandes, Leandro Santoro
Vetore-Neto, Alberto
Díaz, Ingrit E. Collantes
Santana, Fernanda Carvalho de
Mancini-Filho, Jorge
Bacchi, Elfriede Marianne
dc.subject.por.fl_str_mv Gastric ulcer/study/rats
Gastric ulcer/treatment
Kalanchoe pinnata/chromatographic profile
Kalanchoe pinnata/gastroprotective activity
Quercetrin
Quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside.
topic Gastric ulcer/study/rats
Gastric ulcer/treatment
Kalanchoe pinnata/chromatographic profile
Kalanchoe pinnata/gastroprotective activity
Quercetrin
Quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside.
description Peptic ulcers are an important pathology, and the search for safer and more effective treatment methods is of paramount importance. In this study, we assess the gastroprotective effects of the hydroethanolic extract (HE) and ethyl acetate fraction (EAF) from Kalanchoe pinnata leaves against an ethanol/HCl-induced ulcer model in rats. The HE reduced gastric lesions by approximately 47% (400 mg/kg). A significant inhibition of the gastric lesions by 50% was observed after pretreatment with the EAF (200 mg/kg). Quercetrin and quercetin 3-O-α-L-arabinopyranosyl-(1→2)-α-L-rhamnopyranoside were isolated and identified in the flavonoid fraction (EAF) by HPLC and NMR analyses because this fraction showed the highest gastroprotective effect. This fraction demonstrated high antioxidant activities (CE50=41.91 µg/mL) by DPPH in comparison with Trolox(r) and 11.33 mmol Trolox(r) equivalent by ORAC. In conclusion, the HE and FAE from K. pinnata displayed gastroprotective activity in rats, most likely due to the presence of flavonoids.
publishDate 2017
dc.date.none.fl_str_mv 2017-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/131427
10.1590/s2175-97902017000116027
url https://www.revistas.usp.br/bjps/article/view/131427
identifier_str_mv 10.1590/s2175-97902017000116027
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/131427/127807
dc.rights.driver.fl_str_mv Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 1 (2017); e16027-
Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 1 (2017); e16027-
Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 1 (2017); e16027-
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
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