Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/153877 |
Resumo: | In the present study we have studied the effect of 25, 50, 75 and 100 µM of luteolin on the transgenic Drosophila expressing human alpha synuclein. The doses of luteolin were established in diet and the PD flies were allowed to feed on it for 24 days. After 24 days of exposure the flies were assayed for climbing assay, oxidative stress markers, caspase-3 & 9 activity and dopamine content. The immunohistochemistry was also performed on the brain sections for the activity of tyrosine hydroxylase. The exposure of luteolin showed a dose dependent delay in the loss of climbing ability and activity, reduction in oxidative stress markers, caspase-3&9 activities and results in an increase in the dopamine content. The results obtained for the immunohistochemistry also supports the protective role of luteolin against the damage of the dopaminergic neurons. |
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Brazilian Journal of Pharmaceutical Sciences |
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Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s diseaseLuteolinParkinson’s diseaseDrosophilaOxidative stressIn the present study we have studied the effect of 25, 50, 75 and 100 µM of luteolin on the transgenic Drosophila expressing human alpha synuclein. The doses of luteolin were established in diet and the PD flies were allowed to feed on it for 24 days. After 24 days of exposure the flies were assayed for climbing assay, oxidative stress markers, caspase-3 & 9 activity and dopamine content. The immunohistochemistry was also performed on the brain sections for the activity of tyrosine hydroxylase. The exposure of luteolin showed a dose dependent delay in the loss of climbing ability and activity, reduction in oxidative stress markers, caspase-3&9 activities and results in an increase in the dopamine content. The results obtained for the immunohistochemistry also supports the protective role of luteolin against the damage of the dopaminergic neurons.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2018-11-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/15387710.1590/s2175-97902018000317760Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 3 (2018); e17760Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 3 (2018); e17760Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 3 (2018); e177602175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/153877/150224Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessSiddique, Yasir HasanJyoti, SmitaNaz, Falaq2019-03-17T13:19:41Zoai:revistas.usp.br:article/153877Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2019-03-17T13:19:41Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease |
title |
Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease |
spellingShingle |
Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease Siddique, Yasir Hasan Luteolin Parkinson’s disease Drosophila Oxidative stress |
title_short |
Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease |
title_full |
Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease |
title_fullStr |
Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease |
title_full_unstemmed |
Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease |
title_sort |
Protective effect of luteolin on the transgenic Drosophila model of Parkinson’s disease |
author |
Siddique, Yasir Hasan |
author_facet |
Siddique, Yasir Hasan Jyoti, Smita Naz, Falaq |
author_role |
author |
author2 |
Jyoti, Smita Naz, Falaq |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Siddique, Yasir Hasan Jyoti, Smita Naz, Falaq |
dc.subject.por.fl_str_mv |
Luteolin Parkinson’s disease Drosophila Oxidative stress |
topic |
Luteolin Parkinson’s disease Drosophila Oxidative stress |
description |
In the present study we have studied the effect of 25, 50, 75 and 100 µM of luteolin on the transgenic Drosophila expressing human alpha synuclein. The doses of luteolin were established in diet and the PD flies were allowed to feed on it for 24 days. After 24 days of exposure the flies were assayed for climbing assay, oxidative stress markers, caspase-3 & 9 activity and dopamine content. The immunohistochemistry was also performed on the brain sections for the activity of tyrosine hydroxylase. The exposure of luteolin showed a dose dependent delay in the loss of climbing ability and activity, reduction in oxidative stress markers, caspase-3&9 activities and results in an increase in the dopamine content. The results obtained for the immunohistochemistry also supports the protective role of luteolin against the damage of the dopaminergic neurons. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-11-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/153877 10.1590/s2175-97902018000317760 |
url |
https://www.revistas.usp.br/bjps/article/view/153877 |
identifier_str_mv |
10.1590/s2175-97902018000317760 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/153877/150224 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 3 (2018); e17760 Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 3 (2018); e17760 Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 3 (2018); e17760 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222913819836416 |