HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand

Detalhes bibliográficos
Autor(a) principal: Sunthudlakhar, Punjaporn Sunthudlakhar
Data de Publicação: 2022
Outros Autores: Sithisarn, Pongtip, Rojsanga, Piyanuch, Jarikasem, Siripen
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Pharmaceutical Sciences
Texto Completo: https://www.revistas.usp.br/bjps/article/view/205850
Resumo: Phellinus mushrooms have been traditionally used for various medicinal purposes. Protocatechuic acid, which was previously reported to be a component in some Phellinus mushrooms, has some pharmacological effects. This study aimed to validate a HPLC method for the quantitative analysis of the protocatechuic acid contents in the extracts from different Phellinus mushroom species collected in Thailand. HPLC was carried out using a C18 column and the gradient mobile phases of 0.1% formic acid in water and 0.1% formic acid in acetonitrile. Method validation was performed to assure the linearity, accuracy, precision, limit of detection and limit of quantitation of the analytical method. The linearity range of protocatechuic acid was 1 - 10 µg/ml. The average recovery was 104.16%. The method was shown to be precise with the RSD of repeatability and intermediate precision at less than 3%. The protocatechuic contents in 11 Phellinus mushrooms were in the range of less than 0.0099 - 0.4121 %w/w of the extract. The developed HPLC method was reliable and suitable for the quantitative analysis of protocatechuic acid content in Phellinus mushrooms.
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spelling HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in ThailandPhellinus mushroomProtocatechuic acidHPLCQuantitative analysisValidationPhellinus mushrooms have been traditionally used for various medicinal purposes. Protocatechuic acid, which was previously reported to be a component in some Phellinus mushrooms, has some pharmacological effects. This study aimed to validate a HPLC method for the quantitative analysis of the protocatechuic acid contents in the extracts from different Phellinus mushroom species collected in Thailand. HPLC was carried out using a C18 column and the gradient mobile phases of 0.1% formic acid in water and 0.1% formic acid in acetonitrile. Method validation was performed to assure the linearity, accuracy, precision, limit of detection and limit of quantitation of the analytical method. The linearity range of protocatechuic acid was 1 - 10 µg/ml. The average recovery was 104.16%. The method was shown to be precise with the RSD of repeatability and intermediate precision at less than 3%. The protocatechuic contents in 11 Phellinus mushrooms were in the range of less than 0.0099 - 0.4121 %w/w of the extract. The developed HPLC method was reliable and suitable for the quantitative analysis of protocatechuic acid content in Phellinus mushrooms.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2022-12-23info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/20585010.1590/s2175-97902022e20656Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022)Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/205850/197174Copyright (c) 2022 Brazilian Journal of Pharmaceutical Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSunthudlakhar, Punjaporn SunthudlakharSithisarn, PongtipRojsanga, PiyanuchJarikasem, Siripen2023-08-23T13:57:09Zoai:revistas.usp.br:article/205850Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2023-08-23T13:57:09Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand
title HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand
spellingShingle HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand
Sunthudlakhar, Punjaporn Sunthudlakhar
Phellinus mushroom
Protocatechuic acid
HPLC
Quantitative analysis
Validation
title_short HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand
title_full HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand
title_fullStr HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand
title_full_unstemmed HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand
title_sort HPLC quantitative analysis of protocatechuic acid contents in 11 Phellinus mushroom species collected in Thailand
author Sunthudlakhar, Punjaporn Sunthudlakhar
author_facet Sunthudlakhar, Punjaporn Sunthudlakhar
Sithisarn, Pongtip
Rojsanga, Piyanuch
Jarikasem, Siripen
author_role author
author2 Sithisarn, Pongtip
Rojsanga, Piyanuch
Jarikasem, Siripen
author2_role author
author
author
dc.contributor.author.fl_str_mv Sunthudlakhar, Punjaporn Sunthudlakhar
Sithisarn, Pongtip
Rojsanga, Piyanuch
Jarikasem, Siripen
dc.subject.por.fl_str_mv Phellinus mushroom
Protocatechuic acid
HPLC
Quantitative analysis
Validation
topic Phellinus mushroom
Protocatechuic acid
HPLC
Quantitative analysis
Validation
description Phellinus mushrooms have been traditionally used for various medicinal purposes. Protocatechuic acid, which was previously reported to be a component in some Phellinus mushrooms, has some pharmacological effects. This study aimed to validate a HPLC method for the quantitative analysis of the protocatechuic acid contents in the extracts from different Phellinus mushroom species collected in Thailand. HPLC was carried out using a C18 column and the gradient mobile phases of 0.1% formic acid in water and 0.1% formic acid in acetonitrile. Method validation was performed to assure the linearity, accuracy, precision, limit of detection and limit of quantitation of the analytical method. The linearity range of protocatechuic acid was 1 - 10 µg/ml. The average recovery was 104.16%. The method was shown to be precise with the RSD of repeatability and intermediate precision at less than 3%. The protocatechuic contents in 11 Phellinus mushrooms were in the range of less than 0.0099 - 0.4121 %w/w of the extract. The developed HPLC method was reliable and suitable for the quantitative analysis of protocatechuic acid content in Phellinus mushrooms.
publishDate 2022
dc.date.none.fl_str_mv 2022-12-23
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/205850
10.1590/s2175-97902022e20656
url https://www.revistas.usp.br/bjps/article/view/205850
identifier_str_mv 10.1590/s2175-97902022e20656
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/205850/197174
dc.rights.driver.fl_str_mv Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
https://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences
https://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)
Brazilian Journal of Pharmaceutical Sciences; v. 58 (2022)
Brazilian Journal of Pharmaceutical Sciences; Vol. 58 (2022)
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
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