Effects of high fat diet on kidney lipid content and the Na,K-ATPase activity
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/146823 |
Resumo: | It is widely known that high fat diet (HFD) can contribute to the advent of health problems. Recent studies have indicated that obesity imposes a hemodynamic overload to the kidneys. In order to further investigate such injuries, two groups of six Swiss mice each were fed with a controlled AIN93G diet or a high fat (AIN93G modified) diet for eight weeks. Blood samples were collected to determine the hormonal, lipid profile, glucose, urea, and creatinine levels. Histopathological and immunohistochemical analysis were carried out to analysis the kidney damage. Fractions of renal membranes were prepared to assess the Na,K-ATPase activity, lipid peroxidation, total cholesterol, and phospholipid content. The results indicated that the blood lipid profile, urea and creatinine was not altered by the HFD. On the other hand, it was observed in HFD diet mice elevated glucose blood levels along with an augment on insulin and a decrease on corticosterone release. HFD provoked a reduction in the diameter of the convoluted tubules and cell volume in Bowman’s capsule and an increased number of positive cells with Na,K-ATPase, but reduced the Na,K-ATPase activity and the cholesterol content in the kidney cell membrane but favored the lipid peroxidation. |
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Brazilian Journal of Pharmaceutical Sciences |
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Effects of high fat diet on kidney lipid content and the Na,K-ATPase activityHigh fat diet/EffectsNaK-ATPaseLipid peroxidationKidneyCell membraneIt is widely known that high fat diet (HFD) can contribute to the advent of health problems. Recent studies have indicated that obesity imposes a hemodynamic overload to the kidneys. In order to further investigate such injuries, two groups of six Swiss mice each were fed with a controlled AIN93G diet or a high fat (AIN93G modified) diet for eight weeks. Blood samples were collected to determine the hormonal, lipid profile, glucose, urea, and creatinine levels. Histopathological and immunohistochemical analysis were carried out to analysis the kidney damage. Fractions of renal membranes were prepared to assess the Na,K-ATPase activity, lipid peroxidation, total cholesterol, and phospholipid content. The results indicated that the blood lipid profile, urea and creatinine was not altered by the HFD. On the other hand, it was observed in HFD diet mice elevated glucose blood levels along with an augment on insulin and a decrease on corticosterone release. HFD provoked a reduction in the diameter of the convoluted tubules and cell volume in Bowman’s capsule and an increased number of positive cells with Na,K-ATPase, but reduced the Na,K-ATPase activity and the cholesterol content in the kidney cell membrane but favored the lipid peroxidation.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2018-06-07info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/14682310.1590/s2175-97902018000117165Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 1 (2018); e17165Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 1 (2018); e17165Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 1 (2018); e171652175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/146823/140352Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessGarcia, Israel José PereiraCézar, Jessica SilvaLemos, Bruno SilvaSilva, Lilian NayraRibeiro, Rosy Iara Maciel de AzambujaSantana, Camilla CamerinoGrillo, Luciano Aparecido MeirelesPinto, Flavia Carmo HortaBuzelle, Samyra LopesCortes, Vanessa FariaSantos, Hérica de LimaSantos, Maria Emilia Soares Martins dosBarbosa, Leandro Augusto2018-06-07T16:31:56Zoai:revistas.usp.br:article/146823Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2018-06-07T16:31:56Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Effects of high fat diet on kidney lipid content and the Na,K-ATPase activity |
title |
Effects of high fat diet on kidney lipid content and the Na,K-ATPase activity |
spellingShingle |
Effects of high fat diet on kidney lipid content and the Na,K-ATPase activity Garcia, Israel José Pereira High fat diet/Effects Na K-ATPase Lipid peroxidation Kidney Cell membrane |
title_short |
Effects of high fat diet on kidney lipid content and the Na,K-ATPase activity |
title_full |
Effects of high fat diet on kidney lipid content and the Na,K-ATPase activity |
title_fullStr |
Effects of high fat diet on kidney lipid content and the Na,K-ATPase activity |
title_full_unstemmed |
Effects of high fat diet on kidney lipid content and the Na,K-ATPase activity |
title_sort |
Effects of high fat diet on kidney lipid content and the Na,K-ATPase activity |
author |
Garcia, Israel José Pereira |
author_facet |
Garcia, Israel José Pereira Cézar, Jessica Silva Lemos, Bruno Silva Silva, Lilian Nayra Ribeiro, Rosy Iara Maciel de Azambuja Santana, Camilla Camerino Grillo, Luciano Aparecido Meireles Pinto, Flavia Carmo Horta Buzelle, Samyra Lopes Cortes, Vanessa Faria Santos, Hérica de Lima Santos, Maria Emilia Soares Martins dos Barbosa, Leandro Augusto |
author_role |
author |
author2 |
Cézar, Jessica Silva Lemos, Bruno Silva Silva, Lilian Nayra Ribeiro, Rosy Iara Maciel de Azambuja Santana, Camilla Camerino Grillo, Luciano Aparecido Meireles Pinto, Flavia Carmo Horta Buzelle, Samyra Lopes Cortes, Vanessa Faria Santos, Hérica de Lima Santos, Maria Emilia Soares Martins dos Barbosa, Leandro Augusto |
author2_role |
author author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Garcia, Israel José Pereira Cézar, Jessica Silva Lemos, Bruno Silva Silva, Lilian Nayra Ribeiro, Rosy Iara Maciel de Azambuja Santana, Camilla Camerino Grillo, Luciano Aparecido Meireles Pinto, Flavia Carmo Horta Buzelle, Samyra Lopes Cortes, Vanessa Faria Santos, Hérica de Lima Santos, Maria Emilia Soares Martins dos Barbosa, Leandro Augusto |
dc.subject.por.fl_str_mv |
High fat diet/Effects Na K-ATPase Lipid peroxidation Kidney Cell membrane |
topic |
High fat diet/Effects Na K-ATPase Lipid peroxidation Kidney Cell membrane |
description |
It is widely known that high fat diet (HFD) can contribute to the advent of health problems. Recent studies have indicated that obesity imposes a hemodynamic overload to the kidneys. In order to further investigate such injuries, two groups of six Swiss mice each were fed with a controlled AIN93G diet or a high fat (AIN93G modified) diet for eight weeks. Blood samples were collected to determine the hormonal, lipid profile, glucose, urea, and creatinine levels. Histopathological and immunohistochemical analysis were carried out to analysis the kidney damage. Fractions of renal membranes were prepared to assess the Na,K-ATPase activity, lipid peroxidation, total cholesterol, and phospholipid content. The results indicated that the blood lipid profile, urea and creatinine was not altered by the HFD. On the other hand, it was observed in HFD diet mice elevated glucose blood levels along with an augment on insulin and a decrease on corticosterone release. HFD provoked a reduction in the diameter of the convoluted tubules and cell volume in Bowman’s capsule and an increased number of positive cells with Na,K-ATPase, but reduced the Na,K-ATPase activity and the cholesterol content in the kidney cell membrane but favored the lipid peroxidation. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-06-07 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/146823 10.1590/s2175-97902018000117165 |
url |
https://www.revistas.usp.br/bjps/article/view/146823 |
identifier_str_mv |
10.1590/s2175-97902018000117165 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/146823/140352 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 54 Núm. 1 (2018); e17165 Brazilian Journal of Pharmaceutical Sciences; v. 54 n. 1 (2018); e17165 Brazilian Journal of Pharmaceutical Sciences; Vol. 54 No. 1 (2018); e17165 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1821325162098720768 |