The influence of dehydroepiandrosterone on effector functions of neutrophils
Autor(a) principal: | |
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Data de Publicação: | 2022 |
Outros Autores: | , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
DOI: | 10.1590/s2175-97902020000419139 |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/204127 |
Resumo: | Dehydroepiandrosterone (DHEA) is a steroid hormone secreted by the adrenal glands, gonads and brain. It is a precursor to sex hormones and also is known to have immune modulatory activity. However, little is known about the relationship between DHEA and neutrophils and thus our study evaluates the influence of DHEA in the effector functions of neutrophils. Human neutrophils were treated in vitro with DHEA and further infected with Salmonella enterica serovar Typhimurium. The treatment of neutrophils with 0.01 μM of DHEA increased the phagocytosis of Salmonella independent of TLR4 as the treatment did not modulate the TLR4 expression. Additionally, DHEA caused a decrease in ROS (reactive oxygen species) production and did not influence the formation of the neutrophil extracellular trap (NET). Steroid treated neutrophils, infected or stimulated with LPS (lipopolysaccharide), showed reduced production of IL-8, compared to untreated cells. Also, the protein levels of p-NFκB were decreased in neutrophils treated with DHEA, and this reduction could explain the reduced levels of IL-8. These results led us to conclude that the steroid hormone DHEA has important modulatory functions in neutrophils. |
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oai:revistas.usp.br:article/204127 |
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Brazilian Journal of Pharmaceutical Sciences |
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The influence of dehydroepiandrosterone on effector functions of neutrophilsDehydroepiandrosterone; Neuroimmunoendocrinology; S. typhimurium; Innate immunity; PhagocytosisDehydroepiandrosterone (DHEA) is a steroid hormone secreted by the adrenal glands, gonads and brain. It is a precursor to sex hormones and also is known to have immune modulatory activity. However, little is known about the relationship between DHEA and neutrophils and thus our study evaluates the influence of DHEA in the effector functions of neutrophils. Human neutrophils were treated in vitro with DHEA and further infected with Salmonella enterica serovar Typhimurium. The treatment of neutrophils with 0.01 μM of DHEA increased the phagocytosis of Salmonella independent of TLR4 as the treatment did not modulate the TLR4 expression. Additionally, DHEA caused a decrease in ROS (reactive oxygen species) production and did not influence the formation of the neutrophil extracellular trap (NET). Steroid treated neutrophils, infected or stimulated with LPS (lipopolysaccharide), showed reduced production of IL-8, compared to untreated cells. Also, the protein levels of p-NFκB were decreased in neutrophils treated with DHEA, and this reduction could explain the reduced levels of IL-8. These results led us to conclude that the steroid hormone DHEA has important modulatory functions in neutrophils.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2022-11-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://www.revistas.usp.br/bjps/article/view/20412710.1590/s2175-97902020000419139Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021)Brazilian Journal of Pharmaceutical Sciences; v. 57 (2021)Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021)2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/204127/187749Copyright (c) 2022 Brazilian Journal of Pharmaceutical Scienceshttps://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessSoares Brauer, VerônicaAlbani Zambuzi, FabianaSobral Espíndola, MilenaPacífico Cavalcanti Neto, MarinaldoKelly Borges Prado, MorganaCardoso, Priscilla MarianeSilva Soares, LuanaJudson Galvao-Lima, LeonardoMachado Leopoldino, AndréiaRibeiro de Barros Cardoso, CristinaGai Frantz, Fabiani2022-11-09T20:06:25Zoai:revistas.usp.br:article/204127Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2022-11-09T20:06:25Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
The influence of dehydroepiandrosterone on effector functions of neutrophils |
title |
The influence of dehydroepiandrosterone on effector functions of neutrophils |
spellingShingle |
The influence of dehydroepiandrosterone on effector functions of neutrophils The influence of dehydroepiandrosterone on effector functions of neutrophils Soares Brauer, Verônica Dehydroepiandrosterone; Neuroimmunoendocrinology; S. typhimurium; Innate immunity; Phagocytosis Soares Brauer, Verônica Dehydroepiandrosterone; Neuroimmunoendocrinology; S. typhimurium; Innate immunity; Phagocytosis |
title_short |
The influence of dehydroepiandrosterone on effector functions of neutrophils |
title_full |
The influence of dehydroepiandrosterone on effector functions of neutrophils |
title_fullStr |
The influence of dehydroepiandrosterone on effector functions of neutrophils The influence of dehydroepiandrosterone on effector functions of neutrophils |
title_full_unstemmed |
The influence of dehydroepiandrosterone on effector functions of neutrophils The influence of dehydroepiandrosterone on effector functions of neutrophils |
title_sort |
The influence of dehydroepiandrosterone on effector functions of neutrophils |
author |
Soares Brauer, Verônica |
author_facet |
Soares Brauer, Verônica Soares Brauer, Verônica Albani Zambuzi, Fabiana Sobral Espíndola, Milena Pacífico Cavalcanti Neto, Marinaldo Kelly Borges Prado, Morgana Cardoso, Priscilla Mariane Silva Soares, Luana Judson Galvao-Lima, Leonardo Machado Leopoldino, Andréia Ribeiro de Barros Cardoso, Cristina Gai Frantz, Fabiani Albani Zambuzi, Fabiana Sobral Espíndola, Milena Pacífico Cavalcanti Neto, Marinaldo Kelly Borges Prado, Morgana Cardoso, Priscilla Mariane Silva Soares, Luana Judson Galvao-Lima, Leonardo Machado Leopoldino, Andréia Ribeiro de Barros Cardoso, Cristina Gai Frantz, Fabiani |
author_role |
author |
author2 |
Albani Zambuzi, Fabiana Sobral Espíndola, Milena Pacífico Cavalcanti Neto, Marinaldo Kelly Borges Prado, Morgana Cardoso, Priscilla Mariane Silva Soares, Luana Judson Galvao-Lima, Leonardo Machado Leopoldino, Andréia Ribeiro de Barros Cardoso, Cristina Gai Frantz, Fabiani |
author2_role |
author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Soares Brauer, Verônica Albani Zambuzi, Fabiana Sobral Espíndola, Milena Pacífico Cavalcanti Neto, Marinaldo Kelly Borges Prado, Morgana Cardoso, Priscilla Mariane Silva Soares, Luana Judson Galvao-Lima, Leonardo Machado Leopoldino, Andréia Ribeiro de Barros Cardoso, Cristina Gai Frantz, Fabiani |
dc.subject.por.fl_str_mv |
Dehydroepiandrosterone; Neuroimmunoendocrinology; S. typhimurium; Innate immunity; Phagocytosis |
topic |
Dehydroepiandrosterone; Neuroimmunoendocrinology; S. typhimurium; Innate immunity; Phagocytosis |
description |
Dehydroepiandrosterone (DHEA) is a steroid hormone secreted by the adrenal glands, gonads and brain. It is a precursor to sex hormones and also is known to have immune modulatory activity. However, little is known about the relationship between DHEA and neutrophils and thus our study evaluates the influence of DHEA in the effector functions of neutrophils. Human neutrophils were treated in vitro with DHEA and further infected with Salmonella enterica serovar Typhimurium. The treatment of neutrophils with 0.01 μM of DHEA increased the phagocytosis of Salmonella independent of TLR4 as the treatment did not modulate the TLR4 expression. Additionally, DHEA caused a decrease in ROS (reactive oxygen species) production and did not influence the formation of the neutrophil extracellular trap (NET). Steroid treated neutrophils, infected or stimulated with LPS (lipopolysaccharide), showed reduced production of IL-8, compared to untreated cells. Also, the protein levels of p-NFκB were decreased in neutrophils treated with DHEA, and this reduction could explain the reduced levels of IL-8. These results led us to conclude that the steroid hormone DHEA has important modulatory functions in neutrophils. |
publishDate |
2022 |
dc.date.none.fl_str_mv |
2022-11-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/204127 10.1590/s2175-97902020000419139 |
url |
https://www.revistas.usp.br/bjps/article/view/204127 |
identifier_str_mv |
10.1590/s2175-97902020000419139 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/204127/187749 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences https://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2022 Brazilian Journal of Pharmaceutical Sciences https://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021) Brazilian Journal of Pharmaceutical Sciences; v. 57 (2021) Brazilian Journal of Pharmaceutical Sciences; Vol. 57 (2021) 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1822179243236261888 |
dc.identifier.doi.none.fl_str_mv |
10.1590/s2175-97902020000419139 |