Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats

Detalhes bibliográficos
Autor(a) principal: Erhirhie, Earnest Oghenesuvwe
Data de Publicação: 2019
Outros Autores: Ilodigwe, Emmanuel Emeka
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Pharmaceutical Sciences
Texto Completo: https://www.revistas.usp.br/bjps/article/view/164771
Resumo: This study evaluated the acute and sub-chronic toxicities of ethanol leaf extract of Dryopteris filix-mas. Acute toxicity and phytochemical tests on ethanol leaf extract were determined. In sub-chronic toxicity test, animals were treated with 62.5, 125, 250 and 500 mg/kg of extract every day for 90 days. Blood samples were collected via retro-orbital puncture for baseline studies and at 31, 61 and 91st days for determination of hematological, kidney and liver function parameters. Liver and kidneys were harvested for histopathology analyses on 91st day. Also, a 28 day recovery study was carried out to determine reversibility in toxicological effects. Phytochemical screening revealed the presence of tannins, phenols, flavonoids, saponins, steroids, alkaloids, terpenoids, reducing sugar and cardiac glycosides. Acute toxicity test did not show toxicity or death at 5000 mg/kg. There was significant (p<0.005) reduction in white blood cell and lymphocyte counts, significant (p<0.05) increase in some liver and kidney biomarkers as well as alterations in liver and kidney histo-architecture on 91st days in animals that were treated with 250 and 500 mg/kg extract. However, toxicities observed on 91st day were reversible in recovery studies. The leaf extract of Dryopteris filix-mas may be hepatotoxic and nephrotoxic when used for long periods.
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spelling Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino ratsDryopteris filix mas Leaf ExtractSub-chronic toxicityAcute toxicityNephrotoxicityHepatotoxicityDryopteris/toxicityToxicity tests/utilizationEthanol/ toxicityThis study evaluated the acute and sub-chronic toxicities of ethanol leaf extract of Dryopteris filix-mas. Acute toxicity and phytochemical tests on ethanol leaf extract were determined. In sub-chronic toxicity test, animals were treated with 62.5, 125, 250 and 500 mg/kg of extract every day for 90 days. Blood samples were collected via retro-orbital puncture for baseline studies and at 31, 61 and 91st days for determination of hematological, kidney and liver function parameters. Liver and kidneys were harvested for histopathology analyses on 91st day. Also, a 28 day recovery study was carried out to determine reversibility in toxicological effects. Phytochemical screening revealed the presence of tannins, phenols, flavonoids, saponins, steroids, alkaloids, terpenoids, reducing sugar and cardiac glycosides. Acute toxicity test did not show toxicity or death at 5000 mg/kg. There was significant (p<0.005) reduction in white blood cell and lymphocyte counts, significant (p<0.05) increase in some liver and kidney biomarkers as well as alterations in liver and kidney histo-architecture on 91st days in animals that were treated with 250 and 500 mg/kg extract. However, toxicities observed on 91st day were reversible in recovery studies. The leaf extract of Dryopteris filix-mas may be hepatotoxic and nephrotoxic when used for long periods.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2019-12-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/16477110.1590/s2175-97902019000118107Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18107Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e18107Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e181072175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/164771/157956Copyright (c) 2019 Brazilian Journal of Pharmaceutical Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessErhirhie, Earnest OghenesuvweIlodigwe, Emmanuel Emeka2021-01-11T18:30:42Zoai:revistas.usp.br:article/164771Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2021-01-11T18:30:42Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats
title Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats
spellingShingle Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats
Erhirhie, Earnest Oghenesuvwe
Dryopteris filix mas Leaf Extract
Sub-chronic toxicity
Acute toxicity
Nephrotoxicity
Hepatotoxicity
Dryopteris/toxicity
Toxicity tests/utilization
Ethanol/ toxicity
title_short Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats
title_full Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats
title_fullStr Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats
title_full_unstemmed Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats
title_sort Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats
author Erhirhie, Earnest Oghenesuvwe
author_facet Erhirhie, Earnest Oghenesuvwe
Ilodigwe, Emmanuel Emeka
author_role author
author2 Ilodigwe, Emmanuel Emeka
author2_role author
dc.contributor.author.fl_str_mv Erhirhie, Earnest Oghenesuvwe
Ilodigwe, Emmanuel Emeka
dc.subject.por.fl_str_mv Dryopteris filix mas Leaf Extract
Sub-chronic toxicity
Acute toxicity
Nephrotoxicity
Hepatotoxicity
Dryopteris/toxicity
Toxicity tests/utilization
Ethanol/ toxicity
topic Dryopteris filix mas Leaf Extract
Sub-chronic toxicity
Acute toxicity
Nephrotoxicity
Hepatotoxicity
Dryopteris/toxicity
Toxicity tests/utilization
Ethanol/ toxicity
description This study evaluated the acute and sub-chronic toxicities of ethanol leaf extract of Dryopteris filix-mas. Acute toxicity and phytochemical tests on ethanol leaf extract were determined. In sub-chronic toxicity test, animals were treated with 62.5, 125, 250 and 500 mg/kg of extract every day for 90 days. Blood samples were collected via retro-orbital puncture for baseline studies and at 31, 61 and 91st days for determination of hematological, kidney and liver function parameters. Liver and kidneys were harvested for histopathology analyses on 91st day. Also, a 28 day recovery study was carried out to determine reversibility in toxicological effects. Phytochemical screening revealed the presence of tannins, phenols, flavonoids, saponins, steroids, alkaloids, terpenoids, reducing sugar and cardiac glycosides. Acute toxicity test did not show toxicity or death at 5000 mg/kg. There was significant (p<0.005) reduction in white blood cell and lymphocyte counts, significant (p<0.05) increase in some liver and kidney biomarkers as well as alterations in liver and kidney histo-architecture on 91st days in animals that were treated with 250 and 500 mg/kg extract. However, toxicities observed on 91st day were reversible in recovery studies. The leaf extract of Dryopteris filix-mas may be hepatotoxic and nephrotoxic when used for long periods.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-04
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/164771
10.1590/s2175-97902019000118107
url https://www.revistas.usp.br/bjps/article/view/164771
identifier_str_mv 10.1590/s2175-97902019000118107
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/164771/157956
dc.rights.driver.fl_str_mv Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18107
Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e18107
Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18107
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
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