Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats
Autor(a) principal: | |
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Data de Publicação: | 2019 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/164771 |
Resumo: | This study evaluated the acute and sub-chronic toxicities of ethanol leaf extract of Dryopteris filix-mas. Acute toxicity and phytochemical tests on ethanol leaf extract were determined. In sub-chronic toxicity test, animals were treated with 62.5, 125, 250 and 500 mg/kg of extract every day for 90 days. Blood samples were collected via retro-orbital puncture for baseline studies and at 31, 61 and 91st days for determination of hematological, kidney and liver function parameters. Liver and kidneys were harvested for histopathology analyses on 91st day. Also, a 28 day recovery study was carried out to determine reversibility in toxicological effects. Phytochemical screening revealed the presence of tannins, phenols, flavonoids, saponins, steroids, alkaloids, terpenoids, reducing sugar and cardiac glycosides. Acute toxicity test did not show toxicity or death at 5000 mg/kg. There was significant (p<0.005) reduction in white blood cell and lymphocyte counts, significant (p<0.05) increase in some liver and kidney biomarkers as well as alterations in liver and kidney histo-architecture on 91st days in animals that were treated with 250 and 500 mg/kg extract. However, toxicities observed on 91st day were reversible in recovery studies. The leaf extract of Dryopteris filix-mas may be hepatotoxic and nephrotoxic when used for long periods. |
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Brazilian Journal of Pharmaceutical Sciences |
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Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino ratsDryopteris filix mas Leaf ExtractSub-chronic toxicityAcute toxicityNephrotoxicityHepatotoxicityDryopteris/toxicityToxicity tests/utilizationEthanol/ toxicityThis study evaluated the acute and sub-chronic toxicities of ethanol leaf extract of Dryopteris filix-mas. Acute toxicity and phytochemical tests on ethanol leaf extract were determined. In sub-chronic toxicity test, animals were treated with 62.5, 125, 250 and 500 mg/kg of extract every day for 90 days. Blood samples were collected via retro-orbital puncture for baseline studies and at 31, 61 and 91st days for determination of hematological, kidney and liver function parameters. Liver and kidneys were harvested for histopathology analyses on 91st day. Also, a 28 day recovery study was carried out to determine reversibility in toxicological effects. Phytochemical screening revealed the presence of tannins, phenols, flavonoids, saponins, steroids, alkaloids, terpenoids, reducing sugar and cardiac glycosides. Acute toxicity test did not show toxicity or death at 5000 mg/kg. There was significant (p<0.005) reduction in white blood cell and lymphocyte counts, significant (p<0.05) increase in some liver and kidney biomarkers as well as alterations in liver and kidney histo-architecture on 91st days in animals that were treated with 250 and 500 mg/kg extract. However, toxicities observed on 91st day were reversible in recovery studies. The leaf extract of Dryopteris filix-mas may be hepatotoxic and nephrotoxic when used for long periods.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2019-12-04info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/16477110.1590/s2175-97902019000118107Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18107Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e18107Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e181072175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/164771/157956Copyright (c) 2019 Brazilian Journal of Pharmaceutical Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessErhirhie, Earnest OghenesuvweIlodigwe, Emmanuel Emeka2021-01-11T18:30:42Zoai:revistas.usp.br:article/164771Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2021-01-11T18:30:42Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats |
title |
Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats |
spellingShingle |
Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats Erhirhie, Earnest Oghenesuvwe Dryopteris filix mas Leaf Extract Sub-chronic toxicity Acute toxicity Nephrotoxicity Hepatotoxicity Dryopteris/toxicity Toxicity tests/utilization Ethanol/ toxicity |
title_short |
Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats |
title_full |
Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats |
title_fullStr |
Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats |
title_full_unstemmed |
Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats |
title_sort |
Sub-chronic toxicity evaluation of Dryopteris filix-mas (L.) schott, leaf extract in albino rats |
author |
Erhirhie, Earnest Oghenesuvwe |
author_facet |
Erhirhie, Earnest Oghenesuvwe Ilodigwe, Emmanuel Emeka |
author_role |
author |
author2 |
Ilodigwe, Emmanuel Emeka |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Erhirhie, Earnest Oghenesuvwe Ilodigwe, Emmanuel Emeka |
dc.subject.por.fl_str_mv |
Dryopteris filix mas Leaf Extract Sub-chronic toxicity Acute toxicity Nephrotoxicity Hepatotoxicity Dryopteris/toxicity Toxicity tests/utilization Ethanol/ toxicity |
topic |
Dryopteris filix mas Leaf Extract Sub-chronic toxicity Acute toxicity Nephrotoxicity Hepatotoxicity Dryopteris/toxicity Toxicity tests/utilization Ethanol/ toxicity |
description |
This study evaluated the acute and sub-chronic toxicities of ethanol leaf extract of Dryopteris filix-mas. Acute toxicity and phytochemical tests on ethanol leaf extract were determined. In sub-chronic toxicity test, animals were treated with 62.5, 125, 250 and 500 mg/kg of extract every day for 90 days. Blood samples were collected via retro-orbital puncture for baseline studies and at 31, 61 and 91st days for determination of hematological, kidney and liver function parameters. Liver and kidneys were harvested for histopathology analyses on 91st day. Also, a 28 day recovery study was carried out to determine reversibility in toxicological effects. Phytochemical screening revealed the presence of tannins, phenols, flavonoids, saponins, steroids, alkaloids, terpenoids, reducing sugar and cardiac glycosides. Acute toxicity test did not show toxicity or death at 5000 mg/kg. There was significant (p<0.005) reduction in white blood cell and lymphocyte counts, significant (p<0.05) increase in some liver and kidney biomarkers as well as alterations in liver and kidney histo-architecture on 91st days in animals that were treated with 250 and 500 mg/kg extract. However, toxicities observed on 91st day were reversible in recovery studies. The leaf extract of Dryopteris filix-mas may be hepatotoxic and nephrotoxic when used for long periods. |
publishDate |
2019 |
dc.date.none.fl_str_mv |
2019-12-04 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/164771 10.1590/s2175-97902019000118107 |
url |
https://www.revistas.usp.br/bjps/article/view/164771 |
identifier_str_mv |
10.1590/s2175-97902019000118107 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/164771/157956 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18107 Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e18107 Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18107 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222914484633600 |