Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/181843 |
Resumo: | T lymphocytes, cytokines, and macrophages play important roles in the clearance of Mycobacterium tuberculosis (Mtb) by the immune system. This study aimed to investigate the effects of isoniazid on the functions of both innate and adaptive immune cells. Healthy rats were randomly divided into experimental and control groups. Each group was randomly divided into three subgroups and named according to the duration of drug feeding, 1, 3, and 3 months followed by drug withdrawal for 1 month. The experimental groups were fed with isoniazid (12 mg/mL) and the control groups with normal saline. The percentage of CD4+ and CD8+ T lymphocytes, level of interleukin (IL)-12 and interferon (IFN)-γ, and function of macrophages were determined at these three time points. Isoniazid significantly increased the percentage of CD4+ T lymphocytes and the CD4+/CD8+ T lymphocyte cell ratio (P < 0.05). It transiently (<1 month) enhanced the functions of rat macrophages significantly (P < 0.05). In summary, isoniazid could increase the percentage of CD4+ T lymphocytes, CD4+/CD8+ T lymphocyte cell ratio, and enhance macrophage function in healthy rats. |
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Brazilian Journal of Pharmaceutical Sciences |
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Influence of isoniazid on T lymphocytes, cytokines, and macrophages in ratsCytokinesImmuneIsoniazidMacrophagesT lymphocytesT lymphocytes, cytokines, and macrophages play important roles in the clearance of Mycobacterium tuberculosis (Mtb) by the immune system. This study aimed to investigate the effects of isoniazid on the functions of both innate and adaptive immune cells. Healthy rats were randomly divided into experimental and control groups. Each group was randomly divided into three subgroups and named according to the duration of drug feeding, 1, 3, and 3 months followed by drug withdrawal for 1 month. The experimental groups were fed with isoniazid (12 mg/mL) and the control groups with normal saline. The percentage of CD4+ and CD8+ T lymphocytes, level of interleukin (IL)-12 and interferon (IFN)-γ, and function of macrophages were determined at these three time points. Isoniazid significantly increased the percentage of CD4+ T lymphocytes and the CD4+/CD8+ T lymphocyte cell ratio (P < 0.05). It transiently (<1 month) enhanced the functions of rat macrophages significantly (P < 0.05). In summary, isoniazid could increase the percentage of CD4+ T lymphocytes, CD4+/CD8+ T lymphocyte cell ratio, and enhance macrophage function in healthy rats.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2020-12-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/18184310.1590/s2175-97902019000418547Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18547 Brazilian Journal of Pharmaceutical Sciences; v. 56 (2020); e18547 Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18547 2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/181843/168741Copyright (c) 2020 Brazilian Journal of Pharmaceutical Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessCao, Bianchuan Li, Qiuju Huang, Zhe Huang, Xiufang Zhu, Yihong Xiao, Ke Huang, Fuli Zhong, Li 2021-06-12T19:46:54Zoai:revistas.usp.br:article/181843Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2021-06-12T19:46:54Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats |
title |
Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats |
spellingShingle |
Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats Cao, Bianchuan Cytokines Immune Isoniazid Macrophages T lymphocytes |
title_short |
Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats |
title_full |
Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats |
title_fullStr |
Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats |
title_full_unstemmed |
Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats |
title_sort |
Influence of isoniazid on T lymphocytes, cytokines, and macrophages in rats |
author |
Cao, Bianchuan |
author_facet |
Cao, Bianchuan Li, Qiuju Huang, Zhe Huang, Xiufang Zhu, Yihong Xiao, Ke Huang, Fuli Zhong, Li |
author_role |
author |
author2 |
Li, Qiuju Huang, Zhe Huang, Xiufang Zhu, Yihong Xiao, Ke Huang, Fuli Zhong, Li |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Cao, Bianchuan Li, Qiuju Huang, Zhe Huang, Xiufang Zhu, Yihong Xiao, Ke Huang, Fuli Zhong, Li |
dc.subject.por.fl_str_mv |
Cytokines Immune Isoniazid Macrophages T lymphocytes |
topic |
Cytokines Immune Isoniazid Macrophages T lymphocytes |
description |
T lymphocytes, cytokines, and macrophages play important roles in the clearance of Mycobacterium tuberculosis (Mtb) by the immune system. This study aimed to investigate the effects of isoniazid on the functions of both innate and adaptive immune cells. Healthy rats were randomly divided into experimental and control groups. Each group was randomly divided into three subgroups and named according to the duration of drug feeding, 1, 3, and 3 months followed by drug withdrawal for 1 month. The experimental groups were fed with isoniazid (12 mg/mL) and the control groups with normal saline. The percentage of CD4+ and CD8+ T lymphocytes, level of interleukin (IL)-12 and interferon (IFN)-γ, and function of macrophages were determined at these three time points. Isoniazid significantly increased the percentage of CD4+ T lymphocytes and the CD4+/CD8+ T lymphocyte cell ratio (P < 0.05). It transiently (<1 month) enhanced the functions of rat macrophages significantly (P < 0.05). In summary, isoniazid could increase the percentage of CD4+ T lymphocytes, CD4+/CD8+ T lymphocyte cell ratio, and enhance macrophage function in healthy rats. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/181843 10.1590/s2175-97902019000418547 |
url |
https://www.revistas.usp.br/bjps/article/view/181843 |
identifier_str_mv |
10.1590/s2175-97902019000418547 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/181843/168741 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18547 Brazilian Journal of Pharmaceutical Sciences; v. 56 (2020); e18547 Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18547 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222915154673664 |