Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract
Autor(a) principal: | |
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Data de Publicação: | 2017 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/134160 |
Resumo: | Hydroxydihydrobovolide (HDB) was for the first time isolated from Portulaca oleracea L. and then its cytotoxicity against SH-SYTY cells was studied. Moreover, a rapid and sensitive ultra-high performance liquid chromatographic (UHPLC) method with bergapten as internal standard (IS) was developed and validated to investigate the pharmacokinetics of HDB in rats after intravenous and oral administrations of extract (POE). The UHPLC analysis was performed on a Diamonsil C18 analytical column, using acetonitrile-water (35:65, v/v) as the mobile phase with UV detection at 220 nm. The calibration curve was linear over the range of 0.2-25 µg/mL in rat plasma. The average extraction recovery was from 90.1 to 98.9%, and the relative standard deviations (RSDs) of the intra- and inter-day precisions were less than 4.7 and 4.1%, respectively. The results showed that 50 µM HDB had significant cytotoxicity on the SH-SY5Y cells, which was rapidly distributed with a Tmax of 11 min after oral administration and presented a low absolute bioavailability, 4.12%. |
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USP-31 |
network_name_str |
Brazilian Journal of Pharmaceutical Sciences |
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Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extractPortulaca oleracea L./hydroxydihydrobovolidePortulaca oleracea L./hydroxydihydrobovolide/cytotoxicityPortulaca oleracea L./hydroxydihydrobovolide/pharmacokineticsHydroxydihydrobovolide/Ultra-high performance liquid chromatography Hydroxydihydrobovolide (HDB) was for the first time isolated from Portulaca oleracea L. and then its cytotoxicity against SH-SYTY cells was studied. Moreover, a rapid and sensitive ultra-high performance liquid chromatographic (UHPLC) method with bergapten as internal standard (IS) was developed and validated to investigate the pharmacokinetics of HDB in rats after intravenous and oral administrations of extract (POE). The UHPLC analysis was performed on a Diamonsil C18 analytical column, using acetonitrile-water (35:65, v/v) as the mobile phase with UV detection at 220 nm. The calibration curve was linear over the range of 0.2-25 µg/mL in rat plasma. The average extraction recovery was from 90.1 to 98.9%, and the relative standard deviations (RSDs) of the intra- and inter-day precisions were less than 4.7 and 4.1%, respectively. The results showed that 50 µM HDB had significant cytotoxicity on the SH-SY5Y cells, which was rapidly distributed with a Tmax of 11 min after oral administration and presented a low absolute bioavailability, 4.12%.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2017-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/13416010.1590/s2175-97902017000216093Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 2 (2017); e16093-Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 2 (2017); e16093-Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 2 (2017); e16093-2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/134160/129978Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso)info:eu-repo/semantics/openAccessXu, LiangTao, XiaojunGao, YucongZhang, WenjieMeng, YihanLi, CuiyuJiang, MingyueXixiang, Ying2017-06-29T17:40:27Zoai:revistas.usp.br:article/134160Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2017-06-29T17:40:27Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
title |
Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
spellingShingle |
Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract Xu, Liang Portulaca oleracea L./hydroxydihydrobovolide Portulaca oleracea L./hydroxydihydrobovolide/cytotoxicity Portulaca oleracea L./hydroxydihydrobovolide/pharmacokinetics Hydroxydihydrobovolide/Ultra-high performance liquid chromatography |
title_short |
Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
title_full |
Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
title_fullStr |
Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
title_full_unstemmed |
Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
title_sort |
Cytotoxicity of hydroxydihydrobovolide and its pharmacokinetic studies in Portulaca oleracea L. extract |
author |
Xu, Liang |
author_facet |
Xu, Liang Tao, Xiaojun Gao, Yucong Zhang, Wenjie Meng, Yihan Li, Cuiyu Jiang, Mingyue Xixiang, Ying |
author_role |
author |
author2 |
Tao, Xiaojun Gao, Yucong Zhang, Wenjie Meng, Yihan Li, Cuiyu Jiang, Mingyue Xixiang, Ying |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Xu, Liang Tao, Xiaojun Gao, Yucong Zhang, Wenjie Meng, Yihan Li, Cuiyu Jiang, Mingyue Xixiang, Ying |
dc.subject.por.fl_str_mv |
Portulaca oleracea L./hydroxydihydrobovolide Portulaca oleracea L./hydroxydihydrobovolide/cytotoxicity Portulaca oleracea L./hydroxydihydrobovolide/pharmacokinetics Hydroxydihydrobovolide/Ultra-high performance liquid chromatography |
topic |
Portulaca oleracea L./hydroxydihydrobovolide Portulaca oleracea L./hydroxydihydrobovolide/cytotoxicity Portulaca oleracea L./hydroxydihydrobovolide/pharmacokinetics Hydroxydihydrobovolide/Ultra-high performance liquid chromatography |
description |
Hydroxydihydrobovolide (HDB) was for the first time isolated from Portulaca oleracea L. and then its cytotoxicity against SH-SYTY cells was studied. Moreover, a rapid and sensitive ultra-high performance liquid chromatographic (UHPLC) method with bergapten as internal standard (IS) was developed and validated to investigate the pharmacokinetics of HDB in rats after intravenous and oral administrations of extract (POE). The UHPLC analysis was performed on a Diamonsil C18 analytical column, using acetonitrile-water (35:65, v/v) as the mobile phase with UV detection at 220 nm. The calibration curve was linear over the range of 0.2-25 µg/mL in rat plasma. The average extraction recovery was from 90.1 to 98.9%, and the relative standard deviations (RSDs) of the intra- and inter-day precisions were less than 4.7 and 4.1%, respectively. The results showed that 50 µM HDB had significant cytotoxicity on the SH-SY5Y cells, which was rapidly distributed with a Tmax of 11 min after oral administration and presented a low absolute bioavailability, 4.12%. |
publishDate |
2017 |
dc.date.none.fl_str_mv |
2017-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/134160 10.1590/s2175-97902017000216093 |
url |
https://www.revistas.usp.br/bjps/article/view/134160 |
identifier_str_mv |
10.1590/s2175-97902017000216093 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/134160/129978 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2018 Brazilian Journal of Pharmaceutical Sciences (Impresso) |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 53 Núm. 2 (2017); e16093- Brazilian Journal of Pharmaceutical Sciences; v. 53 n. 2 (2017); e16093- Brazilian Journal of Pharmaceutical Sciences; Vol. 53 No. 2 (2017); e16093- 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1800222913247313920 |