Delay of neuropathic pain sensitization after application of dexamethasone-loaded implant in sciatic nerve-injured rats

Detalhes bibliográficos
Autor(a) principal: Bastos, Leandro Francisco Silva
Data de Publicação: 2019
Outros Autores: Vago, Juliana Priscila, Caux, Thaís Rolla, Costa, Bruna Lopes, Godin, Adriana Martins, Menezes, Raquel Rezende, Pena, Roberta Ribas, Silva-Cunha, Armando, Sousa, Lirlândia Pires, Machado, Renes Resende, Moraes, Márcio Flávio Dutra, Fialho, Sílvia Ligório, Coelho, Márcio Matos
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Pharmaceutical Sciences
Texto Completo: https://www.revistas.usp.br/bjps/article/view/180958
Resumo: Neuroimmune interactions underlying the development of pain sensitization in models of neuropathic pain have been widely studied. In this study, we evaluated the development of allodynia and its reduction associated with peripheral antineuroinflammatory effects induced by a dexamethasone-loaded biodegradable implant. Chronic constriction injury (CCI) of the sciatic nerve was performed in Wistar rats. The electronic von Frey test was applied to assess mechanical allodynia. The dexamethasone-loaded implant was placed perineurally at the moment of CCI or 12 days after surgery. Dorsal root ganglia (DRG; L4-L5) were harvested and nuclear extracts were assayed by Western blot for detection of nuclear factor (NF)-κB p65/RelA translocation. Dexamethasone delivered from the implant delayed the development of allodynia for approximately three weeks in CCI rats when the implantation was performed at day 0, but allodynia was not reversed when the implantation was performed at day 12. NF-κB was activated in CCI rat DRG compared with naïve or sham animals (day 15), and dexamethasone implant inhibited p65/ RelA translocation in CCI rats compared with control. This study demonstrated that the dexamethasoneloaded implant suppresses allodynia development and peripheral neuroinflammation. This device can reduce the potential side effects associated with oral anti-inflammatory drugs.
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spelling Delay of neuropathic pain sensitization after application of dexamethasone-loaded implant in sciatic nerve-injured ratsChronic Constriction InjuryDexamethasone/ pharmacologyDelivery deviceNeuropathic Pain/ chemically inducedNuclear Factor kappa B/ drug effectsNeuralgia/ chemically inducedNFkappa B/ drug effectsSciatic Nerve/ injuriesAbsorbable Implants/ trendsNeuroimmune interactions underlying the development of pain sensitization in models of neuropathic pain have been widely studied. In this study, we evaluated the development of allodynia and its reduction associated with peripheral antineuroinflammatory effects induced by a dexamethasone-loaded biodegradable implant. Chronic constriction injury (CCI) of the sciatic nerve was performed in Wistar rats. The electronic von Frey test was applied to assess mechanical allodynia. The dexamethasone-loaded implant was placed perineurally at the moment of CCI or 12 days after surgery. Dorsal root ganglia (DRG; L4-L5) were harvested and nuclear extracts were assayed by Western blot for detection of nuclear factor (NF)-κB p65/RelA translocation. Dexamethasone delivered from the implant delayed the development of allodynia for approximately three weeks in CCI rats when the implantation was performed at day 0, but allodynia was not reversed when the implantation was performed at day 12. NF-κB was activated in CCI rat DRG compared with naïve or sham animals (day 15), and dexamethasone implant inhibited p65/ RelA translocation in CCI rats compared with control. This study demonstrated that the dexamethasoneloaded implant suppresses allodynia development and peripheral neuroinflammation. This device can reduce the potential side effects associated with oral anti-inflammatory drugs.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2019-12-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/18095810.1590/s2175-97902019000218112Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18112 Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e18112 Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18112 2175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/180958/167980Copyright (c) 2019 Brazilian Journal of Pharmaceutical Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessBastos, Leandro Francisco Silva Vago, Juliana Priscila Caux, Thaís Rolla Costa, Bruna Lopes Godin, Adriana Martins Menezes, Raquel Rezende Pena, Roberta Ribas Silva-Cunha, Armando Sousa, Lirlândia Pires Machado, Renes Resende Moraes, Márcio Flávio Dutra Fialho, Sílvia Ligório Coelho, Márcio Matos 2021-01-19T16:47:29Zoai:revistas.usp.br:article/180958Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2021-01-19T16:47:29Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false
dc.title.none.fl_str_mv Delay of neuropathic pain sensitization after application of dexamethasone-loaded implant in sciatic nerve-injured rats
title Delay of neuropathic pain sensitization after application of dexamethasone-loaded implant in sciatic nerve-injured rats
spellingShingle Delay of neuropathic pain sensitization after application of dexamethasone-loaded implant in sciatic nerve-injured rats
Bastos, Leandro Francisco Silva
Chronic Constriction Injury
Dexamethasone/ pharmacology
Delivery device
Neuropathic Pain/ chemically induced
Nuclear Factor kappa B/ drug effects
Neuralgia/ chemically induced
NFkappa B/ drug effects
Sciatic Nerve/ injuries
Absorbable Implants/ trends
title_short Delay of neuropathic pain sensitization after application of dexamethasone-loaded implant in sciatic nerve-injured rats
title_full Delay of neuropathic pain sensitization after application of dexamethasone-loaded implant in sciatic nerve-injured rats
title_fullStr Delay of neuropathic pain sensitization after application of dexamethasone-loaded implant in sciatic nerve-injured rats
title_full_unstemmed Delay of neuropathic pain sensitization after application of dexamethasone-loaded implant in sciatic nerve-injured rats
title_sort Delay of neuropathic pain sensitization after application of dexamethasone-loaded implant in sciatic nerve-injured rats
author Bastos, Leandro Francisco Silva
author_facet Bastos, Leandro Francisco Silva
Vago, Juliana Priscila
Caux, Thaís Rolla
Costa, Bruna Lopes
Godin, Adriana Martins
Menezes, Raquel Rezende
Pena, Roberta Ribas
Silva-Cunha, Armando
Sousa, Lirlândia Pires
Machado, Renes Resende
Moraes, Márcio Flávio Dutra
Fialho, Sílvia Ligório
Coelho, Márcio Matos
author_role author
author2 Vago, Juliana Priscila
Caux, Thaís Rolla
Costa, Bruna Lopes
Godin, Adriana Martins
Menezes, Raquel Rezende
Pena, Roberta Ribas
Silva-Cunha, Armando
Sousa, Lirlândia Pires
Machado, Renes Resende
Moraes, Márcio Flávio Dutra
Fialho, Sílvia Ligório
Coelho, Márcio Matos
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Bastos, Leandro Francisco Silva
Vago, Juliana Priscila
Caux, Thaís Rolla
Costa, Bruna Lopes
Godin, Adriana Martins
Menezes, Raquel Rezende
Pena, Roberta Ribas
Silva-Cunha, Armando
Sousa, Lirlândia Pires
Machado, Renes Resende
Moraes, Márcio Flávio Dutra
Fialho, Sílvia Ligório
Coelho, Márcio Matos
dc.subject.por.fl_str_mv Chronic Constriction Injury
Dexamethasone/ pharmacology
Delivery device
Neuropathic Pain/ chemically induced
Nuclear Factor kappa B/ drug effects
Neuralgia/ chemically induced
NFkappa B/ drug effects
Sciatic Nerve/ injuries
Absorbable Implants/ trends
topic Chronic Constriction Injury
Dexamethasone/ pharmacology
Delivery device
Neuropathic Pain/ chemically induced
Nuclear Factor kappa B/ drug effects
Neuralgia/ chemically induced
NFkappa B/ drug effects
Sciatic Nerve/ injuries
Absorbable Implants/ trends
description Neuroimmune interactions underlying the development of pain sensitization in models of neuropathic pain have been widely studied. In this study, we evaluated the development of allodynia and its reduction associated with peripheral antineuroinflammatory effects induced by a dexamethasone-loaded biodegradable implant. Chronic constriction injury (CCI) of the sciatic nerve was performed in Wistar rats. The electronic von Frey test was applied to assess mechanical allodynia. The dexamethasone-loaded implant was placed perineurally at the moment of CCI or 12 days after surgery. Dorsal root ganglia (DRG; L4-L5) were harvested and nuclear extracts were assayed by Western blot for detection of nuclear factor (NF)-κB p65/RelA translocation. Dexamethasone delivered from the implant delayed the development of allodynia for approximately three weeks in CCI rats when the implantation was performed at day 0, but allodynia was not reversed when the implantation was performed at day 12. NF-κB was activated in CCI rat DRG compared with naïve or sham animals (day 15), and dexamethasone implant inhibited p65/ RelA translocation in CCI rats compared with control. This study demonstrated that the dexamethasoneloaded implant suppresses allodynia development and peripheral neuroinflammation. This device can reduce the potential side effects associated with oral anti-inflammatory drugs.
publishDate 2019
dc.date.none.fl_str_mv 2019-12-09
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjps/article/view/180958
10.1590/s2175-97902019000218112
url https://www.revistas.usp.br/bjps/article/view/180958
identifier_str_mv 10.1590/s2175-97902019000218112
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjps/article/view/180958/167980
dc.rights.driver.fl_str_mv Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences
http://creativecommons.org/licenses/by/4.0
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Copyright (c) 2019 Brazilian Journal of Pharmaceutical Sciences
http://creativecommons.org/licenses/by/4.0
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Ciências Farmacêuticas
dc.source.none.fl_str_mv Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18112
Brazilian Journal of Pharmaceutical Sciences; v. 55 (2019); e18112
Brazilian Journal of Pharmaceutical Sciences; Vol. 55 (2019); e18112
2175-9790
1984-8250
reponame:Brazilian Journal of Pharmaceutical Sciences
instname:Universidade de São Paulo (USP)
instacron:USP
instname_str Universidade de São Paulo (USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Pharmaceutical Sciences
collection Brazilian Journal of Pharmaceutical Sciences
repository.name.fl_str_mv Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)
repository.mail.fl_str_mv bjps@usp.br||elizabeth.igne@gmail.com
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