Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity
Autor(a) principal: | |
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Data de Publicação: | 2020 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Pharmaceutical Sciences |
Texto Completo: | https://www.revistas.usp.br/bjps/article/view/181625 |
Resumo: | This study investigated the effect of ionic liquid (IL)-microwave assisted extraction (MAE) on γ-oryzanol extraction from rice bran (Oryza sativa L.). The conditions included the concentration of IL, liquid (IL)/ solid (sample) ratio, extraction time, and microwave power. The use of 1-butyl-3-methylimidazolium ([Bmim]BF4) as a selected solvent in IL-MAE method was developed for extraction of γ-oryzanol from rice bran. The Box-Behnken design on four factors with response surface methodology (RSM) was used to optimize the experimental conditions. The optimum conditions for the MAE were a [Bmim]BF4 concentration of 0.7 M, liquid/solid ratio of 15 mL/g, extraction time of 10 minutes, and 30% microwave power with a γ-oryzanol value of 0.41 mg/g. The experimental values agreed with those predicted by the RSM models. A microplate reader method was used to determine the IC50 value in tyrosinase inhibitory activity of the extract. An IC50 value of the extract obtained from IL-MAE was 1240 µg/mL. This study suggests that IL-MAE is suitable for the extraction of γ-oryzanol from rice bran and that under the optimum conditions the extract has low activity as a tyrosinase inhibitor. |
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oai:revistas.usp.br:article/181625 |
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USP-31 |
network_name_str |
Brazilian Journal of Pharmaceutical Sciences |
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Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity[Bmim]BF4. Ionic liquidIL-MAEγ-oryzanolTyrosinaseThis study investigated the effect of ionic liquid (IL)-microwave assisted extraction (MAE) on γ-oryzanol extraction from rice bran (Oryza sativa L.). The conditions included the concentration of IL, liquid (IL)/ solid (sample) ratio, extraction time, and microwave power. The use of 1-butyl-3-methylimidazolium ([Bmim]BF4) as a selected solvent in IL-MAE method was developed for extraction of γ-oryzanol from rice bran. The Box-Behnken design on four factors with response surface methodology (RSM) was used to optimize the experimental conditions. The optimum conditions for the MAE were a [Bmim]BF4 concentration of 0.7 M, liquid/solid ratio of 15 mL/g, extraction time of 10 minutes, and 30% microwave power with a γ-oryzanol value of 0.41 mg/g. The experimental values agreed with those predicted by the RSM models. A microplate reader method was used to determine the IC50 value in tyrosinase inhibitory activity of the extract. An IC50 value of the extract obtained from IL-MAE was 1240 µg/mL. This study suggests that IL-MAE is suitable for the extraction of γ-oryzanol from rice bran and that under the optimum conditions the extract has low activity as a tyrosinase inhibitor.Universidade de São Paulo. Faculdade de Ciências Farmacêuticas2020-12-09info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjps/article/view/18162510.1590/s2175-97902019000418115Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18115Brazilian Journal of Pharmaceutical Sciences; v. 56 (2020); e18115Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e181152175-97901984-8250reponame:Brazilian Journal of Pharmaceutical Sciencesinstname:Universidade de São Paulo (USP)instacron:USPenghttps://www.revistas.usp.br/bjps/article/view/181625/168585Copyright (c) 2020 Brazilian Journal of Pharmaceutical Scienceshttp://creativecommons.org/licenses/by/4.0info:eu-repo/semantics/openAccessArrahman, Arif Sigalingging, Efendy Trinovita, Elsa Saputri, Fadlina Chany Mun’im, Abdul 2021-06-12T19:46:54Zoai:revistas.usp.br:article/181625Revistahttps://www.revistas.usp.br/bjps/indexPUBhttps://old.scielo.br/oai/scielo-oai.phpbjps@usp.br||elizabeth.igne@gmail.com2175-97901984-8250opendoar:2021-06-12T19:46:54Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP)false |
dc.title.none.fl_str_mv |
Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity |
title |
Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity |
spellingShingle |
Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity Arrahman, Arif [Bmim]BF4. Ionic liquid IL-MAE γ-oryzanol Tyrosinase |
title_short |
Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity |
title_full |
Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity |
title_fullStr |
Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity |
title_full_unstemmed |
Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity |
title_sort |
Utilization of [Bmim]BF4-MAE on enhancement of γ-oryzanol extraction from rice bran and its tyrosinase inhibitory activity |
author |
Arrahman, Arif |
author_facet |
Arrahman, Arif Sigalingging, Efendy Trinovita, Elsa Saputri, Fadlina Chany Mun’im, Abdul |
author_role |
author |
author2 |
Sigalingging, Efendy Trinovita, Elsa Saputri, Fadlina Chany Mun’im, Abdul |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Arrahman, Arif Sigalingging, Efendy Trinovita, Elsa Saputri, Fadlina Chany Mun’im, Abdul |
dc.subject.por.fl_str_mv |
[Bmim]BF4. Ionic liquid IL-MAE γ-oryzanol Tyrosinase |
topic |
[Bmim]BF4. Ionic liquid IL-MAE γ-oryzanol Tyrosinase |
description |
This study investigated the effect of ionic liquid (IL)-microwave assisted extraction (MAE) on γ-oryzanol extraction from rice bran (Oryza sativa L.). The conditions included the concentration of IL, liquid (IL)/ solid (sample) ratio, extraction time, and microwave power. The use of 1-butyl-3-methylimidazolium ([Bmim]BF4) as a selected solvent in IL-MAE method was developed for extraction of γ-oryzanol from rice bran. The Box-Behnken design on four factors with response surface methodology (RSM) was used to optimize the experimental conditions. The optimum conditions for the MAE were a [Bmim]BF4 concentration of 0.7 M, liquid/solid ratio of 15 mL/g, extraction time of 10 minutes, and 30% microwave power with a γ-oryzanol value of 0.41 mg/g. The experimental values agreed with those predicted by the RSM models. A microplate reader method was used to determine the IC50 value in tyrosinase inhibitory activity of the extract. An IC50 value of the extract obtained from IL-MAE was 1240 µg/mL. This study suggests that IL-MAE is suitable for the extraction of γ-oryzanol from rice bran and that under the optimum conditions the extract has low activity as a tyrosinase inhibitor. |
publishDate |
2020 |
dc.date.none.fl_str_mv |
2020-12-09 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/181625 10.1590/s2175-97902019000418115 |
url |
https://www.revistas.usp.br/bjps/article/view/181625 |
identifier_str_mv |
10.1590/s2175-97902019000418115 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjps/article/view/181625/168585 |
dc.rights.driver.fl_str_mv |
Copyright (c) 2020 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 info:eu-repo/semantics/openAccess |
rights_invalid_str_mv |
Copyright (c) 2020 Brazilian Journal of Pharmaceutical Sciences http://creativecommons.org/licenses/by/4.0 |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Ciências Farmacêuticas |
dc.source.none.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18115 Brazilian Journal of Pharmaceutical Sciences; v. 56 (2020); e18115 Brazilian Journal of Pharmaceutical Sciences; Vol. 56 (2020); e18115 2175-9790 1984-8250 reponame:Brazilian Journal of Pharmaceutical Sciences instname:Universidade de São Paulo (USP) instacron:USP |
instname_str |
Universidade de São Paulo (USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Pharmaceutical Sciences |
collection |
Brazilian Journal of Pharmaceutical Sciences |
repository.name.fl_str_mv |
Brazilian Journal of Pharmaceutical Sciences - Universidade de São Paulo (USP) |
repository.mail.fl_str_mv |
bjps@usp.br||elizabeth.igne@gmail.com |
_version_ |
1787713372417949696 |