Serum and plasma vascular endothelial growth factor in healthy dogs

Detalhes bibliográficos
Autor(a) principal: Queiroz, Genilson Fernandes de
Data de Publicação: 2013
Outros Autores: Franchini, Maria Luiza, Garcez Neto, Américo Froes, Matera, Julia Maria
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/79923
Resumo: Vascular endothelial growth factor (VEGF) is an angiogenic factor with a key role in physiological and pathological process. It can be measured in several organic fluids, including serum and plasma samples. The aim of this work was to investigate the concentration of serum and plasma VEGF of healthy dogs in order to recommend optimal handling of biological samples for accurate measurement of VEGF. Blood samples of thirty dogs were collected into sterile EDTA tube for plasma analysis and into clot activator tubes for serum analysis. The tubes were centrifuged within 90 minutes of collection at 1400 xg for 10 minutes. VEGF concentration was determined using the quantitative method (ELISA). Serum VEGF level was 26.5 + 13.3pg/mL and plasma VEGF was 11.7 + 16.4 pg/mL (p = 0.0003). There was a positive correlation between serum VEGF and platelets (r = 0.37, p = 0.03) and a negative correlation between serum VEGF and hemoglobin (r = -0.38, p = 0.03) and between plasma VEGF and  hemoglobin (r = -0.34, p = 0.06). When compared with serum samples it was concluded that plasma samples could be used as an optimal fluid for measuring VEGF in dogs.
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spelling Serum and plasma vascular endothelial growth factor in healthy dogsFator de crescimento do endotélio vascular no soro e plasma de cães saudáveisAngiogêneseFator de crescimentoFluidos orgânicosAngiogenesisGrowth factorOrganic fluidsVascular endothelial growth factor (VEGF) is an angiogenic factor with a key role in physiological and pathological process. It can be measured in several organic fluids, including serum and plasma samples. The aim of this work was to investigate the concentration of serum and plasma VEGF of healthy dogs in order to recommend optimal handling of biological samples for accurate measurement of VEGF. Blood samples of thirty dogs were collected into sterile EDTA tube for plasma analysis and into clot activator tubes for serum analysis. The tubes were centrifuged within 90 minutes of collection at 1400 xg for 10 minutes. VEGF concentration was determined using the quantitative method (ELISA). Serum VEGF level was 26.5 + 13.3pg/mL and plasma VEGF was 11.7 + 16.4 pg/mL (p = 0.0003). There was a positive correlation between serum VEGF and platelets (r = 0.37, p = 0.03) and a negative correlation between serum VEGF and hemoglobin (r = -0.38, p = 0.03) and between plasma VEGF and  hemoglobin (r = -0.34, p = 0.06). When compared with serum samples it was concluded that plasma samples could be used as an optimal fluid for measuring VEGF in dogs.O fator de crescimento do endotélio vascular (VEGF) é um fator angiogênico com papel importante em processos patológicos e fisiológicos. O VEGF pode ser quantificado em diversos fluidos orgânicos, incluindo amostras de soro e plasma. O presente trabalho investigou a concentração do VEGF no soro e no plasma de cães saudáveis a fim de recomendar o manejo ótimo de amostras biológicas para a determinação dos níveis do VEGF. Amostras de sangue de 30 cães saudáveis foram coletadas em tubos estéreis contendo EDTA para análise do plasma e em tubos com ativador de coagulação para análise do soro. Os tubos foram centrifugados após 90 minutos da coleta a 1.400 x g por 10 minutos. A concentração do VEGF foi determinada com o método quantitativo de ELISA. O nível sérico médio de VEGF foi de 26,5+13,3pg/mL e o plasmático foi 11,7 + 16.4 pg/mL (p = 0,0003). Houve correlação positiva entre o VEGF do soro com as plaquetas (r = 0,37, p = 0,03) e correlação negativa entre o VEGF do soro com hemoglobina (r = -0,38, p = 0,03) e entre VEGF do plasma com hemoglobina (r = -0,34, p = 0,06). A comparação dos resultado obtidos nos exames de plasma e soro indicou que amostras de plasma podem ser utilizadas como ótimo fluido para quantificação do VEGF de cães.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2013-10-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/7992310.11606/issn.2318-3659.v50i5p359-363Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 5 (2013); 359-363Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 5 (2013); 359-363Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 5 (2013); 359-363Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 5 (2013); 359-3631678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/79923/83911Queiroz, Genilson Fernandes deFranchini, Maria LuizaGarcez Neto, Américo FroesMatera, Julia Mariainfo:eu-repo/semantics/openAccess2020-06-23T04:06:57Zoai:revistas.usp.br:article/79923Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:51.720413Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Serum and plasma vascular endothelial growth factor in healthy dogs
Fator de crescimento do endotélio vascular no soro e plasma de cães saudáveis
title Serum and plasma vascular endothelial growth factor in healthy dogs
spellingShingle Serum and plasma vascular endothelial growth factor in healthy dogs
Queiroz, Genilson Fernandes de
Angiogênese
Fator de crescimento
Fluidos orgânicos
Angiogenesis
Growth factor
Organic fluids
title_short Serum and plasma vascular endothelial growth factor in healthy dogs
title_full Serum and plasma vascular endothelial growth factor in healthy dogs
title_fullStr Serum and plasma vascular endothelial growth factor in healthy dogs
title_full_unstemmed Serum and plasma vascular endothelial growth factor in healthy dogs
title_sort Serum and plasma vascular endothelial growth factor in healthy dogs
author Queiroz, Genilson Fernandes de
author_facet Queiroz, Genilson Fernandes de
Franchini, Maria Luiza
Garcez Neto, Américo Froes
Matera, Julia Maria
author_role author
author2 Franchini, Maria Luiza
Garcez Neto, Américo Froes
Matera, Julia Maria
author2_role author
author
author
dc.contributor.author.fl_str_mv Queiroz, Genilson Fernandes de
Franchini, Maria Luiza
Garcez Neto, Américo Froes
Matera, Julia Maria
dc.subject.por.fl_str_mv Angiogênese
Fator de crescimento
Fluidos orgânicos
Angiogenesis
Growth factor
Organic fluids
topic Angiogênese
Fator de crescimento
Fluidos orgânicos
Angiogenesis
Growth factor
Organic fluids
description Vascular endothelial growth factor (VEGF) is an angiogenic factor with a key role in physiological and pathological process. It can be measured in several organic fluids, including serum and plasma samples. The aim of this work was to investigate the concentration of serum and plasma VEGF of healthy dogs in order to recommend optimal handling of biological samples for accurate measurement of VEGF. Blood samples of thirty dogs were collected into sterile EDTA tube for plasma analysis and into clot activator tubes for serum analysis. The tubes were centrifuged within 90 minutes of collection at 1400 xg for 10 minutes. VEGF concentration was determined using the quantitative method (ELISA). Serum VEGF level was 26.5 + 13.3pg/mL and plasma VEGF was 11.7 + 16.4 pg/mL (p = 0.0003). There was a positive correlation between serum VEGF and platelets (r = 0.37, p = 0.03) and a negative correlation between serum VEGF and hemoglobin (r = -0.38, p = 0.03) and between plasma VEGF and  hemoglobin (r = -0.34, p = 0.06). When compared with serum samples it was concluded that plasma samples could be used as an optimal fluid for measuring VEGF in dogs.
publishDate 2013
dc.date.none.fl_str_mv 2013-10-29
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/79923
10.11606/issn.2318-3659.v50i5p359-363
url https://www.revistas.usp.br/bjvras/article/view/79923
identifier_str_mv 10.11606/issn.2318-3659.v50i5p359-363
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/79923/83911
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 5 (2013); 359-363
Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 5 (2013); 359-363
Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 5 (2013); 359-363
Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 5 (2013); 359-363
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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