Serum and plasma vascular endothelial growth factor in healthy dogs
Autor(a) principal: | |
---|---|
Data de Publicação: | 2013 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/79923 |
Resumo: | Vascular endothelial growth factor (VEGF) is an angiogenic factor with a key role in physiological and pathological process. It can be measured in several organic fluids, including serum and plasma samples. The aim of this work was to investigate the concentration of serum and plasma VEGF of healthy dogs in order to recommend optimal handling of biological samples for accurate measurement of VEGF. Blood samples of thirty dogs were collected into sterile EDTA tube for plasma analysis and into clot activator tubes for serum analysis. The tubes were centrifuged within 90 minutes of collection at 1400 xg for 10 minutes. VEGF concentration was determined using the quantitative method (ELISA). Serum VEGF level was 26.5 + 13.3pg/mL and plasma VEGF was 11.7 + 16.4 pg/mL (p = 0.0003). There was a positive correlation between serum VEGF and platelets (r = 0.37, p = 0.03) and a negative correlation between serum VEGF and hemoglobin (r = -0.38, p = 0.03) and between plasma VEGF and hemoglobin (r = -0.34, p = 0.06). When compared with serum samples it was concluded that plasma samples could be used as an optimal fluid for measuring VEGF in dogs. |
id |
USP-49_0a9e1918363aa8abcafa5098475cad05 |
---|---|
oai_identifier_str |
oai:revistas.usp.br:article/79923 |
network_acronym_str |
USP-49 |
network_name_str |
Brazilian Journal of Veterinary Research and Animal Science |
repository_id_str |
https://www.revistas.usp.br/bjvras/index |
spelling |
Serum and plasma vascular endothelial growth factor in healthy dogsFator de crescimento do endotélio vascular no soro e plasma de cães saudáveisAngiogêneseFator de crescimentoFluidos orgânicosAngiogenesisGrowth factorOrganic fluidsVascular endothelial growth factor (VEGF) is an angiogenic factor with a key role in physiological and pathological process. It can be measured in several organic fluids, including serum and plasma samples. The aim of this work was to investigate the concentration of serum and plasma VEGF of healthy dogs in order to recommend optimal handling of biological samples for accurate measurement of VEGF. Blood samples of thirty dogs were collected into sterile EDTA tube for plasma analysis and into clot activator tubes for serum analysis. The tubes were centrifuged within 90 minutes of collection at 1400 xg for 10 minutes. VEGF concentration was determined using the quantitative method (ELISA). Serum VEGF level was 26.5 + 13.3pg/mL and plasma VEGF was 11.7 + 16.4 pg/mL (p = 0.0003). There was a positive correlation between serum VEGF and platelets (r = 0.37, p = 0.03) and a negative correlation between serum VEGF and hemoglobin (r = -0.38, p = 0.03) and between plasma VEGF and hemoglobin (r = -0.34, p = 0.06). When compared with serum samples it was concluded that plasma samples could be used as an optimal fluid for measuring VEGF in dogs.O fator de crescimento do endotélio vascular (VEGF) é um fator angiogênico com papel importante em processos patológicos e fisiológicos. O VEGF pode ser quantificado em diversos fluidos orgânicos, incluindo amostras de soro e plasma. O presente trabalho investigou a concentração do VEGF no soro e no plasma de cães saudáveis a fim de recomendar o manejo ótimo de amostras biológicas para a determinação dos níveis do VEGF. Amostras de sangue de 30 cães saudáveis foram coletadas em tubos estéreis contendo EDTA para análise do plasma e em tubos com ativador de coagulação para análise do soro. Os tubos foram centrifugados após 90 minutos da coleta a 1.400 x g por 10 minutos. A concentração do VEGF foi determinada com o método quantitativo de ELISA. O nível sérico médio de VEGF foi de 26,5+13,3pg/mL e o plasmático foi 11,7 + 16.4 pg/mL (p = 0,0003). Houve correlação positiva entre o VEGF do soro com as plaquetas (r = 0,37, p = 0,03) e correlação negativa entre o VEGF do soro com hemoglobina (r = -0,38, p = 0,03) e entre VEGF do plasma com hemoglobina (r = -0,34, p = 0,06). A comparação dos resultado obtidos nos exames de plasma e soro indicou que amostras de plasma podem ser utilizadas como ótimo fluido para quantificação do VEGF de cães.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2013-10-29info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/7992310.11606/issn.2318-3659.v50i5p359-363Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 5 (2013); 359-363Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 5 (2013); 359-363Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 5 (2013); 359-363Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 5 (2013); 359-3631678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/79923/83911Queiroz, Genilson Fernandes deFranchini, Maria LuizaGarcez Neto, Américo FroesMatera, Julia Mariainfo:eu-repo/semantics/openAccess2020-06-23T04:06:57Zoai:revistas.usp.br:article/79923Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:51.720413Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Serum and plasma vascular endothelial growth factor in healthy dogs Fator de crescimento do endotélio vascular no soro e plasma de cães saudáveis |
title |
Serum and plasma vascular endothelial growth factor in healthy dogs |
spellingShingle |
Serum and plasma vascular endothelial growth factor in healthy dogs Queiroz, Genilson Fernandes de Angiogênese Fator de crescimento Fluidos orgânicos Angiogenesis Growth factor Organic fluids |
title_short |
Serum and plasma vascular endothelial growth factor in healthy dogs |
title_full |
Serum and plasma vascular endothelial growth factor in healthy dogs |
title_fullStr |
Serum and plasma vascular endothelial growth factor in healthy dogs |
title_full_unstemmed |
Serum and plasma vascular endothelial growth factor in healthy dogs |
title_sort |
Serum and plasma vascular endothelial growth factor in healthy dogs |
author |
Queiroz, Genilson Fernandes de |
author_facet |
Queiroz, Genilson Fernandes de Franchini, Maria Luiza Garcez Neto, Américo Froes Matera, Julia Maria |
author_role |
author |
author2 |
Franchini, Maria Luiza Garcez Neto, Américo Froes Matera, Julia Maria |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Queiroz, Genilson Fernandes de Franchini, Maria Luiza Garcez Neto, Américo Froes Matera, Julia Maria |
dc.subject.por.fl_str_mv |
Angiogênese Fator de crescimento Fluidos orgânicos Angiogenesis Growth factor Organic fluids |
topic |
Angiogênese Fator de crescimento Fluidos orgânicos Angiogenesis Growth factor Organic fluids |
description |
Vascular endothelial growth factor (VEGF) is an angiogenic factor with a key role in physiological and pathological process. It can be measured in several organic fluids, including serum and plasma samples. The aim of this work was to investigate the concentration of serum and plasma VEGF of healthy dogs in order to recommend optimal handling of biological samples for accurate measurement of VEGF. Blood samples of thirty dogs were collected into sterile EDTA tube for plasma analysis and into clot activator tubes for serum analysis. The tubes were centrifuged within 90 minutes of collection at 1400 xg for 10 minutes. VEGF concentration was determined using the quantitative method (ELISA). Serum VEGF level was 26.5 + 13.3pg/mL and plasma VEGF was 11.7 + 16.4 pg/mL (p = 0.0003). There was a positive correlation between serum VEGF and platelets (r = 0.37, p = 0.03) and a negative correlation between serum VEGF and hemoglobin (r = -0.38, p = 0.03) and between plasma VEGF and hemoglobin (r = -0.34, p = 0.06). When compared with serum samples it was concluded that plasma samples could be used as an optimal fluid for measuring VEGF in dogs. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-10-29 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/79923 10.11606/issn.2318-3659.v50i5p359-363 |
url |
https://www.revistas.usp.br/bjvras/article/view/79923 |
identifier_str_mv |
10.11606/issn.2318-3659.v50i5p359-363 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/79923/83911 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 5 (2013); 359-363 Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 5 (2013); 359-363 Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 5 (2013); 359-363 Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 5 (2013); 359-363 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051566516076544 |