Efeito da solução salina 0,9% e tampão fosfato salina em diferentes temperaturas e tempos de incubação sobre a morfologia de folículos pré-antrais caprinos

• Autor(a) principal: Santos, Regiane Rodrigues dos
• Data de Publicação: 2002
• Outros Autores: Silva, José Roberto Viana, Costa, Sonia Helena Furtado, Rodrigues, Ana Paula Ribeiro, Lôbo, Raimundo Nonato Braga, Figueiredo, José Ricardo de
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
• Texto Completo: https://www.revistas.usp.br/bjvras/article/view/5957
• topic: Caprino; Folículo pré-antral; Conservação; Solução salina 0; 9%; PBS; Goat; Preantral follicle; Preservation; 0.9% saline solution; PBS

The present work investigated the efficiency of 0.9% saline solution and Phosphate Buffered Saline (PBS) in the preservation of goat preantral follicles in situ at different temperatures and incubation times. The ovarian pair of each animal was divided into 19 fragments. One ovarian fragment was taken randomly and fixed (control). The other 18 fragments were randomly distributed in tubes containing 0.9% saline solution or PBS at 4, 20 or 39 ºC for 4, 12 or 24 h. A total of 5,921 preantral follicles were examined. The quality of preantral follicles was evaluated by classical histology. The storage of ovarian fragments in 0.9% saline solution or PBS at 4 ºC did not reduce significantly the percentage of morphologically normal follicles when compared with the control, except after preservation in 0.9% saline solution for 24 h. The storage of ovarian fragments at 20 or 39°C reduced the percentage of normal preantral follicles when compared to the control, except after preservation in PBS at 20°C for 4 h. In conclusion, this study showed for the first time that goat preantral follicles can be stored in situ successfully at 4 ºC in 0.9% saline solution for 12 h and in PBS for 24 h, and at 20 ºC in PBS for 4 h.