Transplante de fração total de células mononucleares ou fração vascular estromal associada à membrana celulósica em feridas cutâneas experimentais de coelhos

• Autor(a) principal: Treichel, Tiago Luis Eilers
• Data de Publicação: 2011
• Outros Autores: Cunha, Marina Gabriela Monteiro Carvalho Mori da, Cunha, João Paulo Monteiro Carvalho Mori da, Santos Júnior, Eduardo de Bastos, Leme Júnior, Paulo de Tarso de Oliveira, Costa, Márcio Machado, Pippi, Ney Luis, Lopes, Sônia Teresinha dos Anjos, Fighera, Rafael Almeida, Rosa, Maurício Borges da
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
• Texto Completo: https://www.revistas.usp.br/bjvras/article/view/34377
• topic: Fração de células; Reparo; Ferida; Pele; Coelho; Fraction of cells; Repair; Wound; Skin; Rabbit

The skin presents various important functions to the organism. The maintenance of its integrity is fundamental, among others, to prevent penetration of microorganisms and exit of liquids essential to life maintenance. Due to its constant environment exposure, the skin is highly susceptible to trauma which can result solutions of continuity. The healing of wounded skin should be fast and many alternatives are searched for, aiming to reduce repair time and to guarantee a functional and esthetically acceptable scar. One option to repair injured tissues which is ever more viable is cellular therapy with adult stem cells. The aims of this study consisted in evaluating the transplant of total mononuclear cell fraction (TMCF) from bone marrow (BM) or stromal vascular fraction (SVF) from adipose tissue (AT), associated with the use of cellulose membrane. To carry out this experiment, 20 rabbits were randomly divided in four groups with five animals each. After induction of the experimental cutaneous wound, Group A did not receive any treatment; group B received only cellulose membrane; and groups C and D, in addition to receiving the membrane, were submitted to autologous transplant of TMCF, with total cell value between 6,92 x 10(6) and 4,91 x 10(7) and a viability of 82 to 97% or SVF, with total cell value between 9,6 x 10(5) and 6,5 x 10(6) and a viability of 66 a 87%, respectively. At the end of the evaluation period, the three treated groups presented significant statistical difference of wound area in relation to the control group, and the group which received SVF from adipose tissue presented the shortest wound healing time.