Efeito dos timócitos sobre a reprodução de fêmeas de camundongos "nude"
Autor(a) principal: | |
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Data de Publicação: | 2000 |
Outros Autores: | |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/5857 |
Resumo: | Heterozygous and homozygous nude female mice with the genetic background BALB/c were housed in a clean conventional colony at the Laboratory Animal Facilities of Instituto Butantan. Nude animals are known to have severe deficiencies in reproductive function. Nude female is usually sterile where only 8.8% of them present spontaneous fertility. In order to study the involvement of the thymus for these reproductive defects, athymic nude female at different stages of development were treated with thymocytes suspensions and mated with heterozygous males. The results obtained for nude females at 10 and 30 days of age were respectively: a) number of pregnant = 48% and 12%; b) number offspring per female = 5.0 and 4.0; c) natality rates were 2.40 and 0.48; and d) under conditions used in this study the life span for nude animals was prolonged until 7 months. The use of this treatment would increase the animal numbers, which are important research models for biomedical investigations. Considering that nude mice are difficult to raise, an obtainment of these animals in higher number by this method appear to be an essential event, and might offer to grow better for the animals, which are used for breeding. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Efeito dos timócitos sobre a reprodução de fêmeas de camundongos "nude"Effect of thymocytes on reproductive parameters of nude female miceCamundongos nudeHomozigotosHeterozigotosTimócitosNude miceHomozygotesHeterozygotesThymocytesHeterozygous and homozygous nude female mice with the genetic background BALB/c were housed in a clean conventional colony at the Laboratory Animal Facilities of Instituto Butantan. Nude animals are known to have severe deficiencies in reproductive function. Nude female is usually sterile where only 8.8% of them present spontaneous fertility. In order to study the involvement of the thymus for these reproductive defects, athymic nude female at different stages of development were treated with thymocytes suspensions and mated with heterozygous males. The results obtained for nude females at 10 and 30 days of age were respectively: a) number of pregnant = 48% and 12%; b) number offspring per female = 5.0 and 4.0; c) natality rates were 2.40 and 0.48; and d) under conditions used in this study the life span for nude animals was prolonged until 7 months. The use of this treatment would increase the animal numbers, which are important research models for biomedical investigations. Considering that nude mice are difficult to raise, an obtainment of these animals in higher number by this method appear to be an essential event, and might offer to grow better for the animals, which are used for breeding.A colônia de camundongos da linhagem BALB/c, heterozigotos e homozigotos, foi mantida em condições convencionais no Biotério Experimental do Instituto Butantan. Os camundongos "nude" apresentam severa deficiência no sistema reprodutor. As fêmeas de camundongos "nude" são geralmente estéreis; apenas 8,8% apresentam fertilidade espontânea. Para estudar o envolvimento do timo sobre os defeitos na reprodução em diferentes estágios de desenvolvimento, as fêmeas de camundongos "nude" foram tratadas com suspensões de timócitos, e, em seguida, acasaladas com machos heterozigotos. Os resultados obtidos para fêmeas "nude" com 10 e 30 dias de idade foram respectivamente: a) número de fêmeas prenhes = 48% e 12%; b) número de filhotes por fêmea = 5,0 e 4,0; c) as taxas de natalidade foram 2,40 e 0,48; e d) nas condições utilizadas neste trabalho, a vida média dos camundongos "nude" foi prolongada até 7 meses. Este método descreve o aumento do número de animais "nude", importantes em estudos biológicos. Considerando a dificuldade de obtenção de camundongos "nude" em grande número, o uso deste método poderia contribuir para o melhoramento das condições de vida dos animais utilizados nos acasalamentos.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2000-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/585710.1590/S1413-95962000000600002Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 Núm. 6 (2000); 434-438Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 No. 6 (2000); 434-438Brazilian Journal of Veterinary Research and Animal Science; v. 37 n. 6 (2000); 434-438Brazilian Journal of Veterinary Research and Animal Science; V. 37 N. 6 (2000); 434-4381678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/5857/62543Petricevich, Vera LúciaFerreira, Vera Regina Faragoinfo:eu-repo/semantics/openAccess2020-06-23T04:40:25Zoai:revistas.usp.br:article/5857Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:25.972791Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Efeito dos timócitos sobre a reprodução de fêmeas de camundongos "nude" Effect of thymocytes on reproductive parameters of nude female mice |
title |
Efeito dos timócitos sobre a reprodução de fêmeas de camundongos "nude" |
spellingShingle |
Efeito dos timócitos sobre a reprodução de fêmeas de camundongos "nude" Petricevich, Vera Lúcia Camundongos nude Homozigotos Heterozigotos Timócitos Nude mice Homozygotes Heterozygotes Thymocytes |
title_short |
Efeito dos timócitos sobre a reprodução de fêmeas de camundongos "nude" |
title_full |
Efeito dos timócitos sobre a reprodução de fêmeas de camundongos "nude" |
title_fullStr |
Efeito dos timócitos sobre a reprodução de fêmeas de camundongos "nude" |
title_full_unstemmed |
Efeito dos timócitos sobre a reprodução de fêmeas de camundongos "nude" |
title_sort |
Efeito dos timócitos sobre a reprodução de fêmeas de camundongos "nude" |
author |
Petricevich, Vera Lúcia |
author_facet |
Petricevich, Vera Lúcia Ferreira, Vera Regina Farago |
author_role |
author |
author2 |
Ferreira, Vera Regina Farago |
author2_role |
author |
dc.contributor.author.fl_str_mv |
Petricevich, Vera Lúcia Ferreira, Vera Regina Farago |
dc.subject.por.fl_str_mv |
Camundongos nude Homozigotos Heterozigotos Timócitos Nude mice Homozygotes Heterozygotes Thymocytes |
topic |
Camundongos nude Homozigotos Heterozigotos Timócitos Nude mice Homozygotes Heterozygotes Thymocytes |
description |
Heterozygous and homozygous nude female mice with the genetic background BALB/c were housed in a clean conventional colony at the Laboratory Animal Facilities of Instituto Butantan. Nude animals are known to have severe deficiencies in reproductive function. Nude female is usually sterile where only 8.8% of them present spontaneous fertility. In order to study the involvement of the thymus for these reproductive defects, athymic nude female at different stages of development were treated with thymocytes suspensions and mated with heterozygous males. The results obtained for nude females at 10 and 30 days of age were respectively: a) number of pregnant = 48% and 12%; b) number offspring per female = 5.0 and 4.0; c) natality rates were 2.40 and 0.48; and d) under conditions used in this study the life span for nude animals was prolonged until 7 months. The use of this treatment would increase the animal numbers, which are important research models for biomedical investigations. Considering that nude mice are difficult to raise, an obtainment of these animals in higher number by this method appear to be an essential event, and might offer to grow better for the animals, which are used for breeding. |
publishDate |
2000 |
dc.date.none.fl_str_mv |
2000-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5857 10.1590/S1413-95962000000600002 |
url |
https://www.revistas.usp.br/bjvras/article/view/5857 |
identifier_str_mv |
10.1590/S1413-95962000000600002 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5857/62543 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 Núm. 6 (2000); 434-438 Brazilian Journal of Veterinary Research and Animal Science; Vol. 37 No. 6 (2000); 434-438 Brazilian Journal of Veterinary Research and Animal Science; v. 37 n. 6 (2000); 434-438 Brazilian Journal of Veterinary Research and Animal Science; V. 37 N. 6 (2000); 434-438 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051555293167616 |