Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis

Detalhes bibliográficos
Autor(a) principal: Cavalcanti, Josemara Neves
Data de Publicação: 2002
Outros Autores: Guerra, José Luiz, Gambale, Walderez, Corrêa, Benedito, Paula, Claudete Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/5954
Resumo: Dermatophytosis is a zoonosis in whose etiology the dermatophyte Microsporum canis is frequently involved. The fungus can be transmitted to man by dogs and cats. In the present study, guinea pigs were experimentally inoculated with M. canis and the course of the lesions was evaluated. Inoculation resulted in lesions in 100% of the animals, with a clinical course consisting of an incubation period, an inflammatory and a phase of lesion resolution. The histopathologic evaluation of the skin biopsies revealed the presence of acanthosis, hyperkeratosis, spongiosis and marked dermal edema. M. canis spores and hyphae were detected in histologic sections stained with periodic acid-Schiff. The combination of the fluorescent dyes FD and EB permitted the adequate visualization of viable and dead fungal cells in skin fragments of guinea pigs inoculated with M. canis and the identification of the morphologic characteristics of the cells. This experimental model represents a valuable instrument for the study of the pathogenesis of dermatophytic infection with respect to the evaluation of the efficacy of antifungal drugs, and may also be used for the study of the immunology of dermatophytoses and of dermatophyte morphogenes
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spelling Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canisAspectos histopatológicos e micológicos da infecção experimental de cobaias com Microsporum canisDermatofitoseMicrosporum canisInoculaçãoCobaiasDermatophytosisMicrosporum canisRingwormGuinea pigsDermatophytosis is a zoonosis in whose etiology the dermatophyte Microsporum canis is frequently involved. The fungus can be transmitted to man by dogs and cats. In the present study, guinea pigs were experimentally inoculated with M. canis and the course of the lesions was evaluated. Inoculation resulted in lesions in 100% of the animals, with a clinical course consisting of an incubation period, an inflammatory and a phase of lesion resolution. The histopathologic evaluation of the skin biopsies revealed the presence of acanthosis, hyperkeratosis, spongiosis and marked dermal edema. M. canis spores and hyphae were detected in histologic sections stained with periodic acid-Schiff. The combination of the fluorescent dyes FD and EB permitted the adequate visualization of viable and dead fungal cells in skin fragments of guinea pigs inoculated with M. canis and the identification of the morphologic characteristics of the cells. This experimental model represents a valuable instrument for the study of the pathogenesis of dermatophytic infection with respect to the evaluation of the efficacy of antifungal drugs, and may also be used for the study of the immunology of dermatophytoses and of dermatophyte morphogenesCom frequência o dermatófito Microsporum canis está envolvido na etiologia da dermatofitose, podendo ser transmitido para o homem por cães e gatos. Neste estudo foi procedida inoculação experimental de M. canis em cobaias resultando em lesões em 100 % dos animais e o curso clínico consistiu de período de incubação, fase inflamatória e fase de resolução das lesões. O exame histopatológico de biópsias cutâneas revelou presença de infiltrado neutrofílico e edema; acantose, hiperqueratose e espongiose. Estas lesões tornaram-se menos acentuadas no 30.0 dia pós-inoculação. Esporos e hifas de M. canis foram detectadas em cortes histológicos corados com PAS (Ácido Periódico de Schiff). A combinação dos corantes fluorescentes diacetato de fluoresceína (DF) e brometo de etídio (BE), possibilitou uma adequada visualização das células fúngicas viáveis e não viáveis com evidenciação de seus caracteres morfológicos. Este modelo experimental representa um valioso instrumento para o estudo da patogênese da infecção por dermatófitos, para a avaliação da eficácia de drogas antifúngicas, podendo também ser utilizado em estudos sobre a imunologia das dermatofitoses e na determinação da morfogênese de dermatófitos.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/595410.1590/S1413-95962002000500004Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 Núm. 5 (2002); 238-243Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 No. 5 (2002); 238-243Brazilian Journal of Veterinary Research and Animal Science; v. 39 n. 5 (2002); 238-243Brazilian Journal of Veterinary Research and Animal Science; V. 39 N. 5 (2002); 238-2431678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/5954/7485Cavalcanti, Josemara NevesGuerra, José LuizGambale, WalderezCorrêa, BeneditoPaula, Claudete Rodriguesinfo:eu-repo/semantics/openAccess2020-06-23T04:37:24Zoai:revistas.usp.br:article/5954Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:31.410029Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis
Aspectos histopatológicos e micológicos da infecção experimental de cobaias com Microsporum canis
title Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis
spellingShingle Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis
Cavalcanti, Josemara Neves
Dermatofitose
Microsporum canis
Inoculação
Cobaias
Dermatophytosis
Microsporum canis
Ringworm
Guinea pigs
title_short Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis
title_full Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis
title_fullStr Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis
title_full_unstemmed Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis
title_sort Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis
author Cavalcanti, Josemara Neves
author_facet Cavalcanti, Josemara Neves
Guerra, José Luiz
Gambale, Walderez
Corrêa, Benedito
Paula, Claudete Rodrigues
author_role author
author2 Guerra, José Luiz
Gambale, Walderez
Corrêa, Benedito
Paula, Claudete Rodrigues
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Cavalcanti, Josemara Neves
Guerra, José Luiz
Gambale, Walderez
Corrêa, Benedito
Paula, Claudete Rodrigues
dc.subject.por.fl_str_mv Dermatofitose
Microsporum canis
Inoculação
Cobaias
Dermatophytosis
Microsporum canis
Ringworm
Guinea pigs
topic Dermatofitose
Microsporum canis
Inoculação
Cobaias
Dermatophytosis
Microsporum canis
Ringworm
Guinea pigs
description Dermatophytosis is a zoonosis in whose etiology the dermatophyte Microsporum canis is frequently involved. The fungus can be transmitted to man by dogs and cats. In the present study, guinea pigs were experimentally inoculated with M. canis and the course of the lesions was evaluated. Inoculation resulted in lesions in 100% of the animals, with a clinical course consisting of an incubation period, an inflammatory and a phase of lesion resolution. The histopathologic evaluation of the skin biopsies revealed the presence of acanthosis, hyperkeratosis, spongiosis and marked dermal edema. M. canis spores and hyphae were detected in histologic sections stained with periodic acid-Schiff. The combination of the fluorescent dyes FD and EB permitted the adequate visualization of viable and dead fungal cells in skin fragments of guinea pigs inoculated with M. canis and the identification of the morphologic characteristics of the cells. This experimental model represents a valuable instrument for the study of the pathogenesis of dermatophytic infection with respect to the evaluation of the efficacy of antifungal drugs, and may also be used for the study of the immunology of dermatophytoses and of dermatophyte morphogenes
publishDate 2002
dc.date.none.fl_str_mv 2002-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5954
10.1590/S1413-95962002000500004
url https://www.revistas.usp.br/bjvras/article/view/5954
identifier_str_mv 10.1590/S1413-95962002000500004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5954/7485
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 Núm. 5 (2002); 238-243
Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 No. 5 (2002); 238-243
Brazilian Journal of Veterinary Research and Animal Science; v. 39 n. 5 (2002); 238-243
Brazilian Journal of Veterinary Research and Animal Science; V. 39 N. 5 (2002); 238-243
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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