Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis
Autor(a) principal: | |
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Data de Publicação: | 2002 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/5954 |
Resumo: | Dermatophytosis is a zoonosis in whose etiology the dermatophyte Microsporum canis is frequently involved. The fungus can be transmitted to man by dogs and cats. In the present study, guinea pigs were experimentally inoculated with M. canis and the course of the lesions was evaluated. Inoculation resulted in lesions in 100% of the animals, with a clinical course consisting of an incubation period, an inflammatory and a phase of lesion resolution. The histopathologic evaluation of the skin biopsies revealed the presence of acanthosis, hyperkeratosis, spongiosis and marked dermal edema. M. canis spores and hyphae were detected in histologic sections stained with periodic acid-Schiff. The combination of the fluorescent dyes FD and EB permitted the adequate visualization of viable and dead fungal cells in skin fragments of guinea pigs inoculated with M. canis and the identification of the morphologic characteristics of the cells. This experimental model represents a valuable instrument for the study of the pathogenesis of dermatophytic infection with respect to the evaluation of the efficacy of antifungal drugs, and may also be used for the study of the immunology of dermatophytoses and of dermatophyte morphogenes |
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Brazilian Journal of Veterinary Research and Animal Science |
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Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canisAspectos histopatológicos e micológicos da infecção experimental de cobaias com Microsporum canisDermatofitoseMicrosporum canisInoculaçãoCobaiasDermatophytosisMicrosporum canisRingwormGuinea pigsDermatophytosis is a zoonosis in whose etiology the dermatophyte Microsporum canis is frequently involved. The fungus can be transmitted to man by dogs and cats. In the present study, guinea pigs were experimentally inoculated with M. canis and the course of the lesions was evaluated. Inoculation resulted in lesions in 100% of the animals, with a clinical course consisting of an incubation period, an inflammatory and a phase of lesion resolution. The histopathologic evaluation of the skin biopsies revealed the presence of acanthosis, hyperkeratosis, spongiosis and marked dermal edema. M. canis spores and hyphae were detected in histologic sections stained with periodic acid-Schiff. The combination of the fluorescent dyes FD and EB permitted the adequate visualization of viable and dead fungal cells in skin fragments of guinea pigs inoculated with M. canis and the identification of the morphologic characteristics of the cells. This experimental model represents a valuable instrument for the study of the pathogenesis of dermatophytic infection with respect to the evaluation of the efficacy of antifungal drugs, and may also be used for the study of the immunology of dermatophytoses and of dermatophyte morphogenesCom frequência o dermatófito Microsporum canis está envolvido na etiologia da dermatofitose, podendo ser transmitido para o homem por cães e gatos. Neste estudo foi procedida inoculação experimental de M. canis em cobaias resultando em lesões em 100 % dos animais e o curso clínico consistiu de período de incubação, fase inflamatória e fase de resolução das lesões. O exame histopatológico de biópsias cutâneas revelou presença de infiltrado neutrofílico e edema; acantose, hiperqueratose e espongiose. Estas lesões tornaram-se menos acentuadas no 30.0 dia pós-inoculação. Esporos e hifas de M. canis foram detectadas em cortes histológicos corados com PAS (Ácido Periódico de Schiff). A combinação dos corantes fluorescentes diacetato de fluoresceína (DF) e brometo de etídio (BE), possibilitou uma adequada visualização das células fúngicas viáveis e não viáveis com evidenciação de seus caracteres morfológicos. Este modelo experimental representa um valioso instrumento para o estudo da patogênese da infecção por dermatófitos, para a avaliação da eficácia de drogas antifúngicas, podendo também ser utilizado em estudos sobre a imunologia das dermatofitoses e na determinação da morfogênese de dermatófitos.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2002-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/595410.1590/S1413-95962002000500004Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 Núm. 5 (2002); 238-243Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 No. 5 (2002); 238-243Brazilian Journal of Veterinary Research and Animal Science; v. 39 n. 5 (2002); 238-243Brazilian Journal of Veterinary Research and Animal Science; V. 39 N. 5 (2002); 238-2431678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/5954/7485Cavalcanti, Josemara NevesGuerra, José LuizGambale, WalderezCorrêa, BeneditoPaula, Claudete Rodriguesinfo:eu-repo/semantics/openAccess2020-06-23T04:37:24Zoai:revistas.usp.br:article/5954Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:31.410029Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis Aspectos histopatológicos e micológicos da infecção experimental de cobaias com Microsporum canis |
title |
Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis |
spellingShingle |
Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis Cavalcanti, Josemara Neves Dermatofitose Microsporum canis Inoculação Cobaias Dermatophytosis Microsporum canis Ringworm Guinea pigs |
title_short |
Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis |
title_full |
Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis |
title_fullStr |
Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis |
title_full_unstemmed |
Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis |
title_sort |
Histopathologic and mycologic aspects of experimental infection of guinea pigs with Microsporum canis |
author |
Cavalcanti, Josemara Neves |
author_facet |
Cavalcanti, Josemara Neves Guerra, José Luiz Gambale, Walderez Corrêa, Benedito Paula, Claudete Rodrigues |
author_role |
author |
author2 |
Guerra, José Luiz Gambale, Walderez Corrêa, Benedito Paula, Claudete Rodrigues |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Cavalcanti, Josemara Neves Guerra, José Luiz Gambale, Walderez Corrêa, Benedito Paula, Claudete Rodrigues |
dc.subject.por.fl_str_mv |
Dermatofitose Microsporum canis Inoculação Cobaias Dermatophytosis Microsporum canis Ringworm Guinea pigs |
topic |
Dermatofitose Microsporum canis Inoculação Cobaias Dermatophytosis Microsporum canis Ringworm Guinea pigs |
description |
Dermatophytosis is a zoonosis in whose etiology the dermatophyte Microsporum canis is frequently involved. The fungus can be transmitted to man by dogs and cats. In the present study, guinea pigs were experimentally inoculated with M. canis and the course of the lesions was evaluated. Inoculation resulted in lesions in 100% of the animals, with a clinical course consisting of an incubation period, an inflammatory and a phase of lesion resolution. The histopathologic evaluation of the skin biopsies revealed the presence of acanthosis, hyperkeratosis, spongiosis and marked dermal edema. M. canis spores and hyphae were detected in histologic sections stained with periodic acid-Schiff. The combination of the fluorescent dyes FD and EB permitted the adequate visualization of viable and dead fungal cells in skin fragments of guinea pigs inoculated with M. canis and the identification of the morphologic characteristics of the cells. This experimental model represents a valuable instrument for the study of the pathogenesis of dermatophytic infection with respect to the evaluation of the efficacy of antifungal drugs, and may also be used for the study of the immunology of dermatophytoses and of dermatophyte morphogenes |
publishDate |
2002 |
dc.date.none.fl_str_mv |
2002-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5954 10.1590/S1413-95962002000500004 |
url |
https://www.revistas.usp.br/bjvras/article/view/5954 |
identifier_str_mv |
10.1590/S1413-95962002000500004 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5954/7485 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 Núm. 5 (2002); 238-243 Brazilian Journal of Veterinary Research and Animal Science; Vol. 39 No. 5 (2002); 238-243 Brazilian Journal of Veterinary Research and Animal Science; v. 39 n. 5 (2002); 238-243 Brazilian Journal of Veterinary Research and Animal Science; V. 39 N. 5 (2002); 238-243 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051555875127296 |