Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/26729 |
Resumo: | A total of eight Escherichia. coli isolates from psittacine birds were serogrouping and investigated for the virulence factors: pili associated with pyelonephritis (pap), S fimbriae (sfa), afimbrial adhesin (afa), capsule K1 (neu), curli fibers (crl, csgA), temperature-sensitive hemagglutinin (tsh), heat labile (LT) and heat stable (STa and STb) enterotoxins, Shiga-like toxins (Stx1 and Stx2), Cytotoxic necrotizing factor (cnf1), haemolysin (hly), aerobactin production (iuc) and serum resistance (iss). The results showed that the isolates belonged to six serogroups: O23; O54; O64; O76; O128 and O152. The found virulence genes were: crl+ in all isolates, pap+, iuc+ and iss+ in three isolates; tsh+ in two isolates. All strains were negative for genes neu, csgA, sfa, afa, hly, cnf and LT, STa, STb, Stx1, Stx2 toxins. Our findings suggested that some E. coli isolated from psittacine birds present some virulence factors of avian pathogenic E. coli (APEC) pathotype. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosisCaracterização molecular dos fatores de virulência de estirpes de Escherichia coli isoladas de papagaios com colibacilose aviáriaPapagaiosEscherichia coliFatores de virulênciaAPECColibaciloseParrotsEscherichia coliVirulence factorsAPECColibacilosisA total of eight Escherichia. coli isolates from psittacine birds were serogrouping and investigated for the virulence factors: pili associated with pyelonephritis (pap), S fimbriae (sfa), afimbrial adhesin (afa), capsule K1 (neu), curli fibers (crl, csgA), temperature-sensitive hemagglutinin (tsh), heat labile (LT) and heat stable (STa and STb) enterotoxins, Shiga-like toxins (Stx1 and Stx2), Cytotoxic necrotizing factor (cnf1), haemolysin (hly), aerobactin production (iuc) and serum resistance (iss). The results showed that the isolates belonged to six serogroups: O23; O54; O64; O76; O128 and O152. The found virulence genes were: crl+ in all isolates, pap+, iuc+ and iss+ in three isolates; tsh+ in two isolates. All strains were negative for genes neu, csgA, sfa, afa, hly, cnf and LT, STa, STb, Stx1, Stx2 toxins. Our findings suggested that some E. coli isolated from psittacine birds present some virulence factors of avian pathogenic E. coli (APEC) pathotype.Oito amostras de Escherichia coli isoladas de papagaios com colibacilose aviária foram sorogrupadas e investigadas para a presença dos fatores de virulência: pili associado a pielonefrite (pap), fímbria S (sfa), adesina afimbrial (afa), cápsula K1 (neu), curli (crl, csgA), hemaglutinina termosensível (tsh), enterotoxinas termo-lábil (LT) e termo-estável (STa e STb), Shiga-like toxinas (Stx1 e Stx2), Fator citotóxico necrotizante (cnf1), hemolisina (hly), aerobactina (iuc) e resistência sérica (iss). Os resultados mostraram que os isolados pertenciam a seis sorogrupos: O23; O54; O64; O76; O128 e O152. Os genes de virulência detectados foram: crl+ em todos os isolados; pap+; iss+ e iuc+ em três isolados, tsh+ em dois isolados. Todas as amostras foram negativas para os genes neu, csgA, sfa, afa, hly, cnf e para as toxinas LT, STa, STb, Stx1 e Stx2. Estes resultados sugerem que amostras de E. coli isoladas de papagaios apresentam alguns fatores de virulência das amostras do patotipo de E. coli patogênica para aves (APEC).Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2672910.11606/S1413-95962008000700007Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 (2008): Suplemento; 54-60Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 (2008): Suplemento; 54-60Brazilian Journal of Veterinary Research and Animal Science; v. 45 (2008): Suplemento; 54-60Brazilian Journal of Veterinary Research and Animal Science; V. 45 (2008): Suplemento; 54-601678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/26729/28512Knöbl, TerezinhaGodoy, Silvia NeryMatushima, Eliana ReikoGuimarães, Marta BritoFerreira, Antonio José Piantinoinfo:eu-repo/semantics/openAccess2020-06-23T04:12:50Zoai:revistas.usp.br:article/26729Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:00.449074Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis Caracterização molecular dos fatores de virulência de estirpes de Escherichia coli isoladas de papagaios com colibacilose aviária |
title |
Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis |
spellingShingle |
Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis Knöbl, Terezinha Papagaios Escherichia coli Fatores de virulência APEC Colibacilose Parrots Escherichia coli Virulence factors APEC Colibacilosis |
title_short |
Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis |
title_full |
Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis |
title_fullStr |
Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis |
title_full_unstemmed |
Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis |
title_sort |
Molecular characterization of virulence factors in Escherichia coli strains isolated from parrots with colibacilosis |
author |
Knöbl, Terezinha |
author_facet |
Knöbl, Terezinha Godoy, Silvia Nery Matushima, Eliana Reiko Guimarães, Marta Brito Ferreira, Antonio José Piantino |
author_role |
author |
author2 |
Godoy, Silvia Nery Matushima, Eliana Reiko Guimarães, Marta Brito Ferreira, Antonio José Piantino |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Knöbl, Terezinha Godoy, Silvia Nery Matushima, Eliana Reiko Guimarães, Marta Brito Ferreira, Antonio José Piantino |
dc.subject.por.fl_str_mv |
Papagaios Escherichia coli Fatores de virulência APEC Colibacilose Parrots Escherichia coli Virulence factors APEC Colibacilosis |
topic |
Papagaios Escherichia coli Fatores de virulência APEC Colibacilose Parrots Escherichia coli Virulence factors APEC Colibacilosis |
description |
A total of eight Escherichia. coli isolates from psittacine birds were serogrouping and investigated for the virulence factors: pili associated with pyelonephritis (pap), S fimbriae (sfa), afimbrial adhesin (afa), capsule K1 (neu), curli fibers (crl, csgA), temperature-sensitive hemagglutinin (tsh), heat labile (LT) and heat stable (STa and STb) enterotoxins, Shiga-like toxins (Stx1 and Stx2), Cytotoxic necrotizing factor (cnf1), haemolysin (hly), aerobactin production (iuc) and serum resistance (iss). The results showed that the isolates belonged to six serogroups: O23; O54; O64; O76; O128 and O152. The found virulence genes were: crl+ in all isolates, pap+, iuc+ and iss+ in three isolates; tsh+ in two isolates. All strains were negative for genes neu, csgA, sfa, afa, hly, cnf and LT, STa, STb, Stx1, Stx2 toxins. Our findings suggested that some E. coli isolated from psittacine birds present some virulence factors of avian pathogenic E. coli (APEC) pathotype. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26729 10.11606/S1413-95962008000700007 |
url |
https://www.revistas.usp.br/bjvras/article/view/26729 |
identifier_str_mv |
10.11606/S1413-95962008000700007 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26729/28512 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 (2008): Suplemento; 54-60 Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 (2008): Suplemento; 54-60 Brazilian Journal of Veterinary Research and Animal Science; v. 45 (2008): Suplemento; 54-60 Brazilian Journal of Veterinary Research and Animal Science; V. 45 (2008): Suplemento; 54-60 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
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1797051558932774912 |