Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis

Detalhes bibliográficos
Autor(a) principal: Pinto, Marcelo Souza
Data de Publicação: 2001
Outros Autores: Faria, José Eurico de, Message, Dejair, Cassini, Sérvio Túlio Alves, Pereira, Carmen Silva, Gioso, Marilú Martins
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/5926
Resumo: In vitro, the sensitivity to different propolis extracts, at a concentration of 100 mg/ml, of Staphylococcus aureus, Staphylococcus sp. coagulase negative, Streptococcus agalactiae and bacteria of the coliform group, isolated from the milk of cows with mastitis, was evaluated using the technique of an agar disk diffusion with a medium doublelayer. The results showed that the commercial propolis, the ethanolic extract, and, in a minor proportion, the methanolic extract inhibited the growth of the Gram positive bacteria, Staphylococcus aureus, Staphylococcus sp. coagulase negative and Streptococcus agalactiae. The extracts obtained through water, etila acetate and chloroform did not inhibit any bacterial strains, nor did the pure ethanol and methanol vehicles that were utilized as controls. The Gram negative bacterium tested, from the coliform group, did not show sensitivity to any extract. Bacterial strains of the same species collected from different sources presented significant differences in sensitivity to the extracts (p < 0.05). In the Streptococcus agalactiae samples, the diameters of the zone of inhibition around the disks were bigger than those observed for samples of Staphylococcus aureus and Staphylococcus sp. coagulase negative. The results of this experiment stimulate the continuation of studies on the use of propolis extracts, by means of using the appropriate vehicles for the treatment of bovine mastitis.
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spelling Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitisEfeito de extratos de própolis verde sobre bactérias patogênicas isoladas do leite de vacas com mastitePrópolisMastiteAgentesAntibacterianosBovinosPropolisMastitisAntibacterialAgentsCattleIn vitro, the sensitivity to different propolis extracts, at a concentration of 100 mg/ml, of Staphylococcus aureus, Staphylococcus sp. coagulase negative, Streptococcus agalactiae and bacteria of the coliform group, isolated from the milk of cows with mastitis, was evaluated using the technique of an agar disk diffusion with a medium doublelayer. The results showed that the commercial propolis, the ethanolic extract, and, in a minor proportion, the methanolic extract inhibited the growth of the Gram positive bacteria, Staphylococcus aureus, Staphylococcus sp. coagulase negative and Streptococcus agalactiae. The extracts obtained through water, etila acetate and chloroform did not inhibit any bacterial strains, nor did the pure ethanol and methanol vehicles that were utilized as controls. The Gram negative bacterium tested, from the coliform group, did not show sensitivity to any extract. Bacterial strains of the same species collected from different sources presented significant differences in sensitivity to the extracts (p < 0.05). In the Streptococcus agalactiae samples, the diameters of the zone of inhibition around the disks were bigger than those observed for samples of Staphylococcus aureus and Staphylococcus sp. coagulase negative. The results of this experiment stimulate the continuation of studies on the use of propolis extracts, by means of using the appropriate vehicles for the treatment of bovine mastitis.A sensibilidade, in vitro, de amostras de Staphylococcus aureus, Staphylococcus sp. coagulase negativos, Streptococcus agalactiae e bactérias do grupo dos coliformes, isoladas do leite de vacas com mastite, a diferentes extratos de própolis, na concentração de 100 mg/ml, foi avaliada pela técnica do antibiograma em discos de papel de filtro com sobrecamada de meio de cultura. Os resultados mostraram que o extrato etanólico de própolis comercial, os extratos etanólico e, em menor proporção, o metanólico inibiram o crescimento das amostras de bactérias Gram-positivas, Staphylococcus aureus, Staphylococcus sp. coagulase negativos e Streptococcus agalactiae. Os extratos obtidos através da água, do acetato de etila e do clorofórmio não inibiram nenhuma amostra bacteriana, assim como os veículos etanol e metanol puros utilizados como controle. A bactéria Gram-negativa testada, do tipo coliforme, não apresentou sensibilidade a nenhum dos extratos. Verificaram-se diferenças significativas (p < 0,05) na sensibilidade aos extratos entre amostras bacterianas de uma mesma espécie, mas de origens diferentes. Nas amostras de Streptococcus agalactiae, os diâmetros dos halos de inibição do crescimento bacteriano ao redor do disco foram maiores que aqueles observados para as amostras de Staphylococcus aureus e Staphylococcus sp. coagulase negativos. Todos estes resultados estimulam o prosseguimento de novas pesquisas sobre a utilização de extratos de própolis, em veículos adequados, com vistas ao tratamento da mastite bovina.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2001-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/592610.1590/S1413-95962001000600006Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 Núm. 6 (2001); 278-283Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 No. 6 (2001); 278-283Brazilian Journal of Veterinary Research and Animal Science; v. 38 n. 6 (2001); 278-283Brazilian Journal of Veterinary Research and Animal Science; V. 38 N. 6 (2001); 278-2831678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/5926/7457Pinto, Marcelo SouzaFaria, José Eurico deMessage, DejairCassini, Sérvio Túlio AlvesPereira, Carmen SilvaGioso, Marilú Martinsinfo:eu-repo/semantics/openAccess2020-06-23T04:38:16Zoai:revistas.usp.br:article/5926Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:29.857718Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis
Efeito de extratos de própolis verde sobre bactérias patogênicas isoladas do leite de vacas com mastite
title Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis
spellingShingle Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis
Pinto, Marcelo Souza
Própolis
Mastite
Agentes
Antibacterianos
Bovinos
Propolis
Mastitis
Antibacterial
Agents
Cattle
title_short Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis
title_full Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis
title_fullStr Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis
title_full_unstemmed Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis
title_sort Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis
author Pinto, Marcelo Souza
author_facet Pinto, Marcelo Souza
Faria, José Eurico de
Message, Dejair
Cassini, Sérvio Túlio Alves
Pereira, Carmen Silva
Gioso, Marilú Martins
author_role author
author2 Faria, José Eurico de
Message, Dejair
Cassini, Sérvio Túlio Alves
Pereira, Carmen Silva
Gioso, Marilú Martins
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Pinto, Marcelo Souza
Faria, José Eurico de
Message, Dejair
Cassini, Sérvio Túlio Alves
Pereira, Carmen Silva
Gioso, Marilú Martins
dc.subject.por.fl_str_mv Própolis
Mastite
Agentes
Antibacterianos
Bovinos
Propolis
Mastitis
Antibacterial
Agents
Cattle
topic Própolis
Mastite
Agentes
Antibacterianos
Bovinos
Propolis
Mastitis
Antibacterial
Agents
Cattle
description In vitro, the sensitivity to different propolis extracts, at a concentration of 100 mg/ml, of Staphylococcus aureus, Staphylococcus sp. coagulase negative, Streptococcus agalactiae and bacteria of the coliform group, isolated from the milk of cows with mastitis, was evaluated using the technique of an agar disk diffusion with a medium doublelayer. The results showed that the commercial propolis, the ethanolic extract, and, in a minor proportion, the methanolic extract inhibited the growth of the Gram positive bacteria, Staphylococcus aureus, Staphylococcus sp. coagulase negative and Streptococcus agalactiae. The extracts obtained through water, etila acetate and chloroform did not inhibit any bacterial strains, nor did the pure ethanol and methanol vehicles that were utilized as controls. The Gram negative bacterium tested, from the coliform group, did not show sensitivity to any extract. Bacterial strains of the same species collected from different sources presented significant differences in sensitivity to the extracts (p < 0.05). In the Streptococcus agalactiae samples, the diameters of the zone of inhibition around the disks were bigger than those observed for samples of Staphylococcus aureus and Staphylococcus sp. coagulase negative. The results of this experiment stimulate the continuation of studies on the use of propolis extracts, by means of using the appropriate vehicles for the treatment of bovine mastitis.
publishDate 2001
dc.date.none.fl_str_mv 2001-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5926
10.1590/S1413-95962001000600006
url https://www.revistas.usp.br/bjvras/article/view/5926
identifier_str_mv 10.1590/S1413-95962001000600006
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5926/7457
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 Núm. 6 (2001); 278-283
Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 No. 6 (2001); 278-283
Brazilian Journal of Veterinary Research and Animal Science; v. 38 n. 6 (2001); 278-283
Brazilian Journal of Veterinary Research and Animal Science; V. 38 N. 6 (2001); 278-283
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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