Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis
Autor(a) principal: | |
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Data de Publicação: | 2001 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/5926 |
Resumo: | In vitro, the sensitivity to different propolis extracts, at a concentration of 100 mg/ml, of Staphylococcus aureus, Staphylococcus sp. coagulase negative, Streptococcus agalactiae and bacteria of the coliform group, isolated from the milk of cows with mastitis, was evaluated using the technique of an agar disk diffusion with a medium doublelayer. The results showed that the commercial propolis, the ethanolic extract, and, in a minor proportion, the methanolic extract inhibited the growth of the Gram positive bacteria, Staphylococcus aureus, Staphylococcus sp. coagulase negative and Streptococcus agalactiae. The extracts obtained through water, etila acetate and chloroform did not inhibit any bacterial strains, nor did the pure ethanol and methanol vehicles that were utilized as controls. The Gram negative bacterium tested, from the coliform group, did not show sensitivity to any extract. Bacterial strains of the same species collected from different sources presented significant differences in sensitivity to the extracts (p < 0.05). In the Streptococcus agalactiae samples, the diameters of the zone of inhibition around the disks were bigger than those observed for samples of Staphylococcus aureus and Staphylococcus sp. coagulase negative. The results of this experiment stimulate the continuation of studies on the use of propolis extracts, by means of using the appropriate vehicles for the treatment of bovine mastitis. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitisEfeito de extratos de própolis verde sobre bactérias patogênicas isoladas do leite de vacas com mastitePrópolisMastiteAgentesAntibacterianosBovinosPropolisMastitisAntibacterialAgentsCattleIn vitro, the sensitivity to different propolis extracts, at a concentration of 100 mg/ml, of Staphylococcus aureus, Staphylococcus sp. coagulase negative, Streptococcus agalactiae and bacteria of the coliform group, isolated from the milk of cows with mastitis, was evaluated using the technique of an agar disk diffusion with a medium doublelayer. The results showed that the commercial propolis, the ethanolic extract, and, in a minor proportion, the methanolic extract inhibited the growth of the Gram positive bacteria, Staphylococcus aureus, Staphylococcus sp. coagulase negative and Streptococcus agalactiae. The extracts obtained through water, etila acetate and chloroform did not inhibit any bacterial strains, nor did the pure ethanol and methanol vehicles that were utilized as controls. The Gram negative bacterium tested, from the coliform group, did not show sensitivity to any extract. Bacterial strains of the same species collected from different sources presented significant differences in sensitivity to the extracts (p < 0.05). In the Streptococcus agalactiae samples, the diameters of the zone of inhibition around the disks were bigger than those observed for samples of Staphylococcus aureus and Staphylococcus sp. coagulase negative. The results of this experiment stimulate the continuation of studies on the use of propolis extracts, by means of using the appropriate vehicles for the treatment of bovine mastitis.A sensibilidade, in vitro, de amostras de Staphylococcus aureus, Staphylococcus sp. coagulase negativos, Streptococcus agalactiae e bactérias do grupo dos coliformes, isoladas do leite de vacas com mastite, a diferentes extratos de própolis, na concentração de 100 mg/ml, foi avaliada pela técnica do antibiograma em discos de papel de filtro com sobrecamada de meio de cultura. Os resultados mostraram que o extrato etanólico de própolis comercial, os extratos etanólico e, em menor proporção, o metanólico inibiram o crescimento das amostras de bactérias Gram-positivas, Staphylococcus aureus, Staphylococcus sp. coagulase negativos e Streptococcus agalactiae. Os extratos obtidos através da água, do acetato de etila e do clorofórmio não inibiram nenhuma amostra bacteriana, assim como os veículos etanol e metanol puros utilizados como controle. A bactéria Gram-negativa testada, do tipo coliforme, não apresentou sensibilidade a nenhum dos extratos. Verificaram-se diferenças significativas (p < 0,05) na sensibilidade aos extratos entre amostras bacterianas de uma mesma espécie, mas de origens diferentes. Nas amostras de Streptococcus agalactiae, os diâmetros dos halos de inibição do crescimento bacteriano ao redor do disco foram maiores que aqueles observados para as amostras de Staphylococcus aureus e Staphylococcus sp. coagulase negativos. Todos estes resultados estimulam o prosseguimento de novas pesquisas sobre a utilização de extratos de própolis, em veículos adequados, com vistas ao tratamento da mastite bovina.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2001-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/592610.1590/S1413-95962001000600006Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 Núm. 6 (2001); 278-283Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 No. 6 (2001); 278-283Brazilian Journal of Veterinary Research and Animal Science; v. 38 n. 6 (2001); 278-283Brazilian Journal of Veterinary Research and Animal Science; V. 38 N. 6 (2001); 278-2831678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/5926/7457Pinto, Marcelo SouzaFaria, José Eurico deMessage, DejairCassini, Sérvio Túlio AlvesPereira, Carmen SilvaGioso, Marilú Martinsinfo:eu-repo/semantics/openAccess2020-06-23T04:38:16Zoai:revistas.usp.br:article/5926Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:29.857718Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis Efeito de extratos de própolis verde sobre bactérias patogênicas isoladas do leite de vacas com mastite |
title |
Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis |
spellingShingle |
Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis Pinto, Marcelo Souza Própolis Mastite Agentes Antibacterianos Bovinos Propolis Mastitis Antibacterial Agents Cattle |
title_short |
Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis |
title_full |
Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis |
title_fullStr |
Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis |
title_full_unstemmed |
Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis |
title_sort |
Effect of green propolis extracts on patogenic bacteria isolated from milk of cows with mastitis |
author |
Pinto, Marcelo Souza |
author_facet |
Pinto, Marcelo Souza Faria, José Eurico de Message, Dejair Cassini, Sérvio Túlio Alves Pereira, Carmen Silva Gioso, Marilú Martins |
author_role |
author |
author2 |
Faria, José Eurico de Message, Dejair Cassini, Sérvio Túlio Alves Pereira, Carmen Silva Gioso, Marilú Martins |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Pinto, Marcelo Souza Faria, José Eurico de Message, Dejair Cassini, Sérvio Túlio Alves Pereira, Carmen Silva Gioso, Marilú Martins |
dc.subject.por.fl_str_mv |
Própolis Mastite Agentes Antibacterianos Bovinos Propolis Mastitis Antibacterial Agents Cattle |
topic |
Própolis Mastite Agentes Antibacterianos Bovinos Propolis Mastitis Antibacterial Agents Cattle |
description |
In vitro, the sensitivity to different propolis extracts, at a concentration of 100 mg/ml, of Staphylococcus aureus, Staphylococcus sp. coagulase negative, Streptococcus agalactiae and bacteria of the coliform group, isolated from the milk of cows with mastitis, was evaluated using the technique of an agar disk diffusion with a medium doublelayer. The results showed that the commercial propolis, the ethanolic extract, and, in a minor proportion, the methanolic extract inhibited the growth of the Gram positive bacteria, Staphylococcus aureus, Staphylococcus sp. coagulase negative and Streptococcus agalactiae. The extracts obtained through water, etila acetate and chloroform did not inhibit any bacterial strains, nor did the pure ethanol and methanol vehicles that were utilized as controls. The Gram negative bacterium tested, from the coliform group, did not show sensitivity to any extract. Bacterial strains of the same species collected from different sources presented significant differences in sensitivity to the extracts (p < 0.05). In the Streptococcus agalactiae samples, the diameters of the zone of inhibition around the disks were bigger than those observed for samples of Staphylococcus aureus and Staphylococcus sp. coagulase negative. The results of this experiment stimulate the continuation of studies on the use of propolis extracts, by means of using the appropriate vehicles for the treatment of bovine mastitis. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5926 10.1590/S1413-95962001000600006 |
url |
https://www.revistas.usp.br/bjvras/article/view/5926 |
identifier_str_mv |
10.1590/S1413-95962001000600006 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5926/7457 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 Núm. 6 (2001); 278-283 Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 No. 6 (2001); 278-283 Brazilian Journal of Veterinary Research and Animal Science; v. 38 n. 6 (2001); 278-283 Brazilian Journal of Veterinary Research and Animal Science; V. 38 N. 6 (2001); 278-283 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
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1797051555808018432 |