Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay

Detalhes bibliográficos
Autor(a) principal: Korndörfer, Clotilde Maria
Data de Publicação: 1998
Outros Autores: Meirelles, Cyro Ferreira, Bueno, Ives Cláudio da Silva, Abdalla, Adibe Luiz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/5705
Resumo: The purpose of this study was to find a practical procedure for the extraction of progesterone (P4) from feces and to determine if the P4 plasma profiles during pregnancy were reflected in the total fecal P4 of pregnant rabbits. The rabbit was used as model for the techniques. Plasma and feces were collected from 11 rabbits during a period of 42 days. Three different methods of P4 extraction were used. The total P4 was measured by solid-phase radioimmunoassay (RIA) with 125I-P4 as the tracer. Results suggested that it was possible to extract total P4 from rabbit feces with methanol and petroleum ether. Plasma and fecal P4 profiles were compared for both pregnant and ovariectomized rabbits. It was possible to differentiate total P4 extracted from day two through 28 after breeding (P<0.01).
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spelling Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassayMétodos de extração para determinação de progesterona em fezes de coelho (Oryctolagus cuniculus) através de radioimunoensaioProgesteroneFecesRadioimmunoassayRabbitProgesteronaFezesRadioimunoensaioCoelhosThe purpose of this study was to find a practical procedure for the extraction of progesterone (P4) from feces and to determine if the P4 plasma profiles during pregnancy were reflected in the total fecal P4 of pregnant rabbits. The rabbit was used as model for the techniques. Plasma and feces were collected from 11 rabbits during a period of 42 days. Three different methods of P4 extraction were used. The total P4 was measured by solid-phase radioimmunoassay (RIA) with 125I-P4 as the tracer. Results suggested that it was possible to extract total P4 from rabbit feces with methanol and petroleum ether. Plasma and fecal P4 profiles were compared for both pregnant and ovariectomized rabbits. It was possible to differentiate total P4 extracted from day two through 28 after breeding (P<0.01).O propósito deste estudo foi determinar uma metodologia mais prática para a extração de progesterona (P4) das fezes e observar se os perfis plasmáticos de progesterona (P4) durante a gestação de coelhas era refletida na concentração total de P4 nas fezes. Esta espécie animal foi utilizada como modelo para as diferentes metodologias. O plasma e as fezes foram coletadas de 11 coelhas, durante período de 42 dias. Três métodos diferentes de extração de P4 foram testados. A P4 total foi medida com auxílio de radioimunoensaio de fase sólida (RIE) utilizando 125I-P4 como traçador. Os resultados sugerem que foi possível extrair P4 total das fezes de coelhas com metanol e éter de petróleo. Os teores de P4 plasmática e fecal foram comparados entre animais gestantes e ovariectomizados. Foi possível a diferenciação (p<0,01) dos teores totais de P4 excretados nas fezes entre a 2º até 28º dia após cobertura.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/570510.1590/S1413-95961998000300004Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 Núm. 3 (1998); 115-119Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 No. 3 (1998); 115-119Brazilian Journal of Veterinary Research and Animal Science; v. 35 n. 3 (1998); 115-119Brazilian Journal of Veterinary Research and Animal Science; V. 35 N. 3 (1998); 115-1191678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/5705/7236Korndörfer, Clotilde MariaMeirelles, Cyro FerreiraBueno, Ives Cláudio da SilvaAbdalla, Adibe Luizinfo:eu-repo/semantics/openAccess2020-06-23T04:45:23Zoai:revistas.usp.br:article/5705Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:18.288983Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay
Métodos de extração para determinação de progesterona em fezes de coelho (Oryctolagus cuniculus) através de radioimunoensaio
title Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay
spellingShingle Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay
Korndörfer, Clotilde Maria
Progesterone
Feces
Radioimmunoassay
Rabbit
Progesterona
Fezes
Radioimunoensaio
Coelhos
title_short Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay
title_full Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay
title_fullStr Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay
title_full_unstemmed Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay
title_sort Evaluation of extraction methods for progesterone determination in rabbit (Oryctolagus cuniculus) feces by radioimmunoassay
author Korndörfer, Clotilde Maria
author_facet Korndörfer, Clotilde Maria
Meirelles, Cyro Ferreira
Bueno, Ives Cláudio da Silva
Abdalla, Adibe Luiz
author_role author
author2 Meirelles, Cyro Ferreira
Bueno, Ives Cláudio da Silva
Abdalla, Adibe Luiz
author2_role author
author
author
dc.contributor.author.fl_str_mv Korndörfer, Clotilde Maria
Meirelles, Cyro Ferreira
Bueno, Ives Cláudio da Silva
Abdalla, Adibe Luiz
dc.subject.por.fl_str_mv Progesterone
Feces
Radioimmunoassay
Rabbit
Progesterona
Fezes
Radioimunoensaio
Coelhos
topic Progesterone
Feces
Radioimmunoassay
Rabbit
Progesterona
Fezes
Radioimunoensaio
Coelhos
description The purpose of this study was to find a practical procedure for the extraction of progesterone (P4) from feces and to determine if the P4 plasma profiles during pregnancy were reflected in the total fecal P4 of pregnant rabbits. The rabbit was used as model for the techniques. Plasma and feces were collected from 11 rabbits during a period of 42 days. Three different methods of P4 extraction were used. The total P4 was measured by solid-phase radioimmunoassay (RIA) with 125I-P4 as the tracer. Results suggested that it was possible to extract total P4 from rabbit feces with methanol and petroleum ether. Plasma and fecal P4 profiles were compared for both pregnant and ovariectomized rabbits. It was possible to differentiate total P4 extracted from day two through 28 after breeding (P<0.01).
publishDate 1998
dc.date.none.fl_str_mv 1998-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5705
10.1590/S1413-95961998000300004
url https://www.revistas.usp.br/bjvras/article/view/5705
identifier_str_mv 10.1590/S1413-95961998000300004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5705/7236
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 Núm. 3 (1998); 115-119
Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 No. 3 (1998); 115-119
Brazilian Journal of Veterinary Research and Animal Science; v. 35 n. 3 (1998); 115-119
Brazilian Journal of Veterinary Research and Animal Science; V. 35 N. 3 (1998); 115-119
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
_version_ 1797051554576990208

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