Laparoscopic approach of the mare as an aid in reproductive assisted technology

Bibliographic Details
Main Author: Fialho, Sergio da Silva
Publication Date: 2001
Other Authors: Figueiró, Giuliano Moraes, Lehmkühl, Ricardo Coelho, Pasin, Marta, Rubin, Mara Iolanda Batistella, Silva, Carlos Antonio Mondino
Format: Article
Language: por
Source: Brazilian Journal of Veterinary Research and Animal Science
Download full: https://www.revistas.usp.br/bjvras/article/view/5876
Summary: Laparoscopy is a poorly explored tool for equine assisted reproduction techniques, despite its use in man, zoo and domestic animals. This study had the objective to evaluate the possibility and offer basis for cannulation of the oviduct and its future application in gamete and embryo transfer programs in this species. Seventeen standing laparoscopies were accomplished in ten mares, each ovary approached from its ipsolateral flank. After identification of the uterine tube, the infundibulum was gently tractioned and a catheter advanced in direction of the abdominal ostium of the uterine tube to the ampulla where 250 µL of culture medium (Dulbecco's - PBS) were deposited. The laparoscopic technique associated with assisted reproduction in the mare can be considered pioneer and have the potential of lowering costs with maintenance of receptors by taking advantage of repetitive cycles, which is impossible by means of the conventional approach through laparotomy. This cost is one of the main obstacles for the commercial application of assisted reproduction techniques in horses.
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spelling Laparoscopic approach of the mare as an aid in reproductive assisted technologyAbordagem laparoscópica na égua como meio auxiliar nas técnicas de reprodução assistidaLaparoscopiaÉguasReproduçãoLaparoscopyMaresReproductionLaparoscopy is a poorly explored tool for equine assisted reproduction techniques, despite its use in man, zoo and domestic animals. This study had the objective to evaluate the possibility and offer basis for cannulation of the oviduct and its future application in gamete and embryo transfer programs in this species. Seventeen standing laparoscopies were accomplished in ten mares, each ovary approached from its ipsolateral flank. After identification of the uterine tube, the infundibulum was gently tractioned and a catheter advanced in direction of the abdominal ostium of the uterine tube to the ampulla where 250 µL of culture medium (Dulbecco's - PBS) were deposited. The laparoscopic technique associated with assisted reproduction in the mare can be considered pioneer and have the potential of lowering costs with maintenance of receptors by taking advantage of repetitive cycles, which is impossible by means of the conventional approach through laparotomy. This cost is one of the main obstacles for the commercial application of assisted reproduction techniques in horses.A laparoscopia é uma ferramenta pouco explorada nas técnicas assistidas da reprodução em eqüinos, ao contrário do que já ocorre com o homem, animais de zoológicos e domésticos. Este estudo objetivou verificar a possibilidade de oferecer bases para a canulação do oviduto e sua futura aplicação em programas de transferência de gametas e embriões nesta espécie. Foram realizadas 17 laparoscopias em 10 éguas mantidas em estação onde cada ovário foi abordado pelo flanco ipsolateral. Após identificação da tuba uterina, o infundibulum foi gentilmente tracionado e um cateter avançado em direção ao óstio abdominal da tuba uterina para a ampola, onde foram depositados 250 µl de meio de cultivo (Dulbecco's - PBS). A utilização da técnica laparoscópica em reprodução assistida na égua pode ser considerada pioneira e tem o potencial de diminuir os custos de manutenção de receptoras através do aproveitamento de ciclos repetidos, o que não é possível pela abordagem convencional através de laparotomia. Este custo é um dos principais entraves à aplicação comercial das técnicas assistidas da reprodução em eqüinos.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2001-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/587610.1590/S1413-95962001000500006Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 Núm. 5 (2001); 229-232Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 No. 5 (2001); 229-232Brazilian Journal of Veterinary Research and Animal Science; v. 38 n. 5 (2001); 229-232Brazilian Journal of Veterinary Research and Animal Science; V. 38 N. 5 (2001); 229-2321678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/5876/7407Fialho, Sergio da SilvaFigueiró, Giuliano MoraesLehmkühl, Ricardo CoelhoPasin, MartaRubin, Mara Iolanda BatistellaSilva, Carlos Antonio Mondinoinfo:eu-repo/semantics/openAccess2020-06-23T04:39:50Zoai:revistas.usp.br:article/5876Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:27.081756Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Laparoscopic approach of the mare as an aid in reproductive assisted technology
Abordagem laparoscópica na égua como meio auxiliar nas técnicas de reprodução assistida
title Laparoscopic approach of the mare as an aid in reproductive assisted technology
spellingShingle Laparoscopic approach of the mare as an aid in reproductive assisted technology
Fialho, Sergio da Silva
Laparoscopia
Éguas
Reprodução
Laparoscopy
Mares
Reproduction
title_short Laparoscopic approach of the mare as an aid in reproductive assisted technology
title_full Laparoscopic approach of the mare as an aid in reproductive assisted technology
title_fullStr Laparoscopic approach of the mare as an aid in reproductive assisted technology
title_full_unstemmed Laparoscopic approach of the mare as an aid in reproductive assisted technology
title_sort Laparoscopic approach of the mare as an aid in reproductive assisted technology
author Fialho, Sergio da Silva
author_facet Fialho, Sergio da Silva
Figueiró, Giuliano Moraes
Lehmkühl, Ricardo Coelho
Pasin, Marta
Rubin, Mara Iolanda Batistella
Silva, Carlos Antonio Mondino
author_role author
author2 Figueiró, Giuliano Moraes
Lehmkühl, Ricardo Coelho
Pasin, Marta
Rubin, Mara Iolanda Batistella
Silva, Carlos Antonio Mondino
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Fialho, Sergio da Silva
Figueiró, Giuliano Moraes
Lehmkühl, Ricardo Coelho
Pasin, Marta
Rubin, Mara Iolanda Batistella
Silva, Carlos Antonio Mondino
dc.subject.por.fl_str_mv Laparoscopia
Éguas
Reprodução
Laparoscopy
Mares
Reproduction
topic Laparoscopia
Éguas
Reprodução
Laparoscopy
Mares
Reproduction
description Laparoscopy is a poorly explored tool for equine assisted reproduction techniques, despite its use in man, zoo and domestic animals. This study had the objective to evaluate the possibility and offer basis for cannulation of the oviduct and its future application in gamete and embryo transfer programs in this species. Seventeen standing laparoscopies were accomplished in ten mares, each ovary approached from its ipsolateral flank. After identification of the uterine tube, the infundibulum was gently tractioned and a catheter advanced in direction of the abdominal ostium of the uterine tube to the ampulla where 250 µL of culture medium (Dulbecco's - PBS) were deposited. The laparoscopic technique associated with assisted reproduction in the mare can be considered pioneer and have the potential of lowering costs with maintenance of receptors by taking advantage of repetitive cycles, which is impossible by means of the conventional approach through laparotomy. This cost is one of the main obstacles for the commercial application of assisted reproduction techniques in horses.
publishDate 2001
dc.date.none.fl_str_mv 2001-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5876
10.1590/S1413-95962001000500006
url https://www.revistas.usp.br/bjvras/article/view/5876
identifier_str_mv 10.1590/S1413-95962001000500006
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5876/7407
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 Núm. 5 (2001); 229-232
Brazilian Journal of Veterinary Research and Animal Science; Vol. 38 No. 5 (2001); 229-232
Brazilian Journal of Veterinary Research and Animal Science; v. 38 n. 5 (2001); 229-232
Brazilian Journal of Veterinary Research and Animal Science; V. 38 N. 5 (2001); 229-232
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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