FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus)
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/26714 |
Resumo: | Present study aimed to evaluate gonadotropins profiles in 12 Nelore heifers, in order to test the hypothesis that FSH concentrations decrease and LH presents a transient increase during follicle selection. Blood samples from jugular vein were harvested twice daily starting at the time of ovulation (D0) until D5. Plasma samples were assayed for FSH and LH by double antibody radioimmunoassay method. LH and FSH assay sensitivity was 0,02ng/ml and 0,005ng/ml, respectively. The intraassay coefficient of variation was 13,6% and 18,8%, respectively. Data (mean±SEM) were normalized to follicle deviation and analyzed by ANOVA and by linear, cubic, and quadratic regressions. Comparisons between higher and lower FSH values were also performed by T-test. There was no effect of time in plasmatic FSH and LH circulating levels when variance analysis or regression analysis were performed. However, by T-test, FSH concentrations reached the lowest plasmatic levels 36 (0,40±0,05ng/ml) and 60 hours (0,42±0,04ng/ml) after follicular deviation, comparatively to 36 hours before deviation, when the concentrations were maximal (0,63±0,08ng/ml). In conclusion, there is a FSH decrease, although a transient LH elevation has not been confirmed encompassing follicle deviation in Nelore females. |
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Brazilian Journal of Veterinary Research and Animal Science |
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FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus)Perfil de FSH e LH na divergência folicular em novilhas Nelore (Bos indicus)Gonadotrofinas hipofisáriasGado NeloreRadioimunoensaioBovinosGonadotropinsPituitaryNeloreRadioimmunoassayCattlePresent study aimed to evaluate gonadotropins profiles in 12 Nelore heifers, in order to test the hypothesis that FSH concentrations decrease and LH presents a transient increase during follicle selection. Blood samples from jugular vein were harvested twice daily starting at the time of ovulation (D0) until D5. Plasma samples were assayed for FSH and LH by double antibody radioimmunoassay method. LH and FSH assay sensitivity was 0,02ng/ml and 0,005ng/ml, respectively. The intraassay coefficient of variation was 13,6% and 18,8%, respectively. Data (mean±SEM) were normalized to follicle deviation and analyzed by ANOVA and by linear, cubic, and quadratic regressions. Comparisons between higher and lower FSH values were also performed by T-test. There was no effect of time in plasmatic FSH and LH circulating levels when variance analysis or regression analysis were performed. However, by T-test, FSH concentrations reached the lowest plasmatic levels 36 (0,40±0,05ng/ml) and 60 hours (0,42±0,04ng/ml) after follicular deviation, comparatively to 36 hours before deviation, when the concentrations were maximal (0,63±0,08ng/ml). In conclusion, there is a FSH decrease, although a transient LH elevation has not been confirmed encompassing follicle deviation in Nelore females.O presente estudo teve como objetivo traçar o perfil de gonadotrofinas em 12 novilhas Nelore, a fim de testar a hipótese de que há declínio nas concentrações de FSH e elevação transitória nos níveis de LH durante a seleção folicular. A partir da ovulação (D0) foram colhidas amostras de sangue da veia jugular a cada 12 horas até o D5, para a dosagem de FSH e LH plasmáticos. Empregou-se o método de radioimunoensaio de duplo anticorpo. A sensibilidade do ensaio de LH foi de 0,02ng/ml e a de FSH de 0,005ng/ml. Os coeficientes de variação intra-ensaio foram 13,6% e 18,8%, respectivamente. Os dados (média±EPM) foram normalizados para o momento da divergência folicular e, posteriormente, analisados por ANOVA e por regressão linear, cúbica e quadrática. Também foi empregado o Teste t para comparação entre o ponto mais alto e o mais baixo da curva. Não se verificou efeito de tempo sobre as concentrações plasmáticas de FSH e de LH quando se utilizou análise de variância e de regressão. Entretanto, através do Teste t pontual, o FSH atingiu as menores concentrações plasmáticas 36 (0,40±0,05ng/ml) e 60 horas (0,42±0,04ng/ml) após a divergência, comparativamente às 36 horas anteriores ao desvio, quando as concentrações foram máximas (0,63±0,08ng/ml). Conclui-se, portanto, que há declínio nas concentrações plasmáticas de FSH, contudo, não foi comprovada elevação transitória nas concentrações de LH próximo ao momento do desvio folicular em fêmeas Nelore.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2008-02-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/2671410.11606/issn.1678-4456.bjvras.2008.26714Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 Núm. 1 (2008); 11-16Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 No. 1 (2008); 11-16Brazilian Journal of Veterinary Research and Animal Science; v. 45 n. 1 (2008); 11-16Brazilian Journal of Veterinary Research and Animal Science; V. 45 N. 1 (2008); 11-161678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/26714/28497Gimenes, Lindsay UnnoSá Filho, Manoel FranciscoTorres-Júnior, José RibamarBeltran, Maria PaulaNogueira, Guilherme de PaulaBaruselli, Pietro Sampaioinfo:eu-repo/semantics/openAccess2020-06-23T04:13:59Zoai:revistas.usp.br:article/26714Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:59.572486Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus) Perfil de FSH e LH na divergência folicular em novilhas Nelore (Bos indicus) |
title |
FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus) |
spellingShingle |
FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus) Gimenes, Lindsay Unno Gonadotrofinas hipofisárias Gado Nelore Radioimunoensaio Bovinos Gonadotropins Pituitary Nelore Radioimmunoassay Cattle |
title_short |
FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus) |
title_full |
FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus) |
title_fullStr |
FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus) |
title_full_unstemmed |
FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus) |
title_sort |
FSH and LH profiles during follicle deviation in Nelore heifers (Bos indicus) |
author |
Gimenes, Lindsay Unno |
author_facet |
Gimenes, Lindsay Unno Sá Filho, Manoel Francisco Torres-Júnior, José Ribamar Beltran, Maria Paula Nogueira, Guilherme de Paula Baruselli, Pietro Sampaio |
author_role |
author |
author2 |
Sá Filho, Manoel Francisco Torres-Júnior, José Ribamar Beltran, Maria Paula Nogueira, Guilherme de Paula Baruselli, Pietro Sampaio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Gimenes, Lindsay Unno Sá Filho, Manoel Francisco Torres-Júnior, José Ribamar Beltran, Maria Paula Nogueira, Guilherme de Paula Baruselli, Pietro Sampaio |
dc.subject.por.fl_str_mv |
Gonadotrofinas hipofisárias Gado Nelore Radioimunoensaio Bovinos Gonadotropins Pituitary Nelore Radioimmunoassay Cattle |
topic |
Gonadotrofinas hipofisárias Gado Nelore Radioimunoensaio Bovinos Gonadotropins Pituitary Nelore Radioimmunoassay Cattle |
description |
Present study aimed to evaluate gonadotropins profiles in 12 Nelore heifers, in order to test the hypothesis that FSH concentrations decrease and LH presents a transient increase during follicle selection. Blood samples from jugular vein were harvested twice daily starting at the time of ovulation (D0) until D5. Plasma samples were assayed for FSH and LH by double antibody radioimmunoassay method. LH and FSH assay sensitivity was 0,02ng/ml and 0,005ng/ml, respectively. The intraassay coefficient of variation was 13,6% and 18,8%, respectively. Data (mean±SEM) were normalized to follicle deviation and analyzed by ANOVA and by linear, cubic, and quadratic regressions. Comparisons between higher and lower FSH values were also performed by T-test. There was no effect of time in plasmatic FSH and LH circulating levels when variance analysis or regression analysis were performed. However, by T-test, FSH concentrations reached the lowest plasmatic levels 36 (0,40±0,05ng/ml) and 60 hours (0,42±0,04ng/ml) after follicular deviation, comparatively to 36 hours before deviation, when the concentrations were maximal (0,63±0,08ng/ml). In conclusion, there is a FSH decrease, although a transient LH elevation has not been confirmed encompassing follicle deviation in Nelore females. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-02-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26714 10.11606/issn.1678-4456.bjvras.2008.26714 |
url |
https://www.revistas.usp.br/bjvras/article/view/26714 |
identifier_str_mv |
10.11606/issn.1678-4456.bjvras.2008.26714 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/26714/28497 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 Núm. 1 (2008); 11-16 Brazilian Journal of Veterinary Research and Animal Science; Vol. 45 No. 1 (2008); 11-16 Brazilian Journal of Veterinary Research and Animal Science; v. 45 n. 1 (2008); 11-16 Brazilian Journal of Veterinary Research and Animal Science; V. 45 N. 1 (2008); 11-16 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051558893977600 |