Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosis

Detalhes bibliográficos
Autor(a) principal: Alcalde, Rosana
Data de Publicação: 2013
Outros Autores: Kogika, Marcia Mery, Fortunato, Vera A. Batistini, Lopes, Luciano Rodrigo, Paiva, Paulo Bandeira, Durigon, Edison Luiz
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/55844
Resumo: Canine distemper virus (CDV) is a pathogen which affects dogs and causes severe disease leading to death. Dogs infected with CDV can be diagnosed by RNA detection by Nested PCR technique. The following study proposed to valuate CDV RNA in blood, urine and saliva samples. The Nested-PCR technique was able to detect CDV RNA in different types of biologic samples. The higher number of positive results was obtained in urine samples.
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spelling Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosisVírus da cinomose canina: detecção do RNA viral pelo Nested RT-PCR em cães com diagnóstico clínicoCinomoseNested-PCRCDVDistemperNested-PCRCDVCanine distemper virus (CDV) is a pathogen which affects dogs and causes severe disease leading to death. Dogs infected with CDV can be diagnosed by RNA detection by Nested PCR technique. The following study proposed to valuate CDV RNA in blood, urine and saliva samples. The Nested-PCR technique was able to detect CDV RNA in different types of biologic samples. The higher number of positive results was obtained in urine samples.O vírus da cinomose canina (CDV) é um patógeno que afeta cães, causando doença grave e que pode levar a morte. Os cães infectados pelo CDV podem ser diagnosticados pela detecção do RNA utilizando-se a técnica de Nested-PCR. O presente estudo teve como objetivo avaliar o RNA do CDV no sangue, urina e saliva em cães com diagnóstico clínico de cinomose. A técnica de Nested-PCR foi capaz de detectar o RNA em diferentes tipos de amostras biológicas. Obteve-se um maior número de resultados positivos em amostras de urina.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia2013-02-22info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5584410.11606/issn.2318-3659.v50i1p74-76Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 1 (2013); 74-76Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 1 (2013); 74-76Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 1 (2013); 74-76Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 1 (2013); 74-761678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPenghttps://www.revistas.usp.br/bjvras/article/view/55844/59247Alcalde, RosanaKogika, Marcia MeryFortunato, Vera A. BatistiniLopes, Luciano RodrigoPaiva, Paulo BandeiraDurigon, Edison Luizinfo:eu-repo/semantics/openAccess2020-06-23T04:07:32Zoai:revistas.usp.br:article/55844Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:32.453382Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosis
Vírus da cinomose canina: detecção do RNA viral pelo Nested RT-PCR em cães com diagnóstico clínico
title Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosis
spellingShingle Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosis
Alcalde, Rosana
Cinomose
Nested-PCR
CDV
Distemper
Nested-PCR
CDV
title_short Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosis
title_full Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosis
title_fullStr Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosis
title_full_unstemmed Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosis
title_sort Canine distemper virus: detection of viral RNA by Nested RT-PCR in dogs with clinical diagnosis
author Alcalde, Rosana
author_facet Alcalde, Rosana
Kogika, Marcia Mery
Fortunato, Vera A. Batistini
Lopes, Luciano Rodrigo
Paiva, Paulo Bandeira
Durigon, Edison Luiz
author_role author
author2 Kogika, Marcia Mery
Fortunato, Vera A. Batistini
Lopes, Luciano Rodrigo
Paiva, Paulo Bandeira
Durigon, Edison Luiz
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Alcalde, Rosana
Kogika, Marcia Mery
Fortunato, Vera A. Batistini
Lopes, Luciano Rodrigo
Paiva, Paulo Bandeira
Durigon, Edison Luiz
dc.subject.por.fl_str_mv Cinomose
Nested-PCR
CDV
Distemper
Nested-PCR
CDV
topic Cinomose
Nested-PCR
CDV
Distemper
Nested-PCR
CDV
description Canine distemper virus (CDV) is a pathogen which affects dogs and causes severe disease leading to death. Dogs infected with CDV can be diagnosed by RNA detection by Nested PCR technique. The following study proposed to valuate CDV RNA in blood, urine and saliva samples. The Nested-PCR technique was able to detect CDV RNA in different types of biologic samples. The higher number of positive results was obtained in urine samples.
publishDate 2013
dc.date.none.fl_str_mv 2013-02-22
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/55844
10.11606/issn.2318-3659.v50i1p74-76
url https://www.revistas.usp.br/bjvras/article/view/55844
identifier_str_mv 10.11606/issn.2318-3659.v50i1p74-76
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/55844/59247
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 Núm. 1 (2013); 74-76
Brazilian Journal of Veterinary Research and Animal Science; Vol. 50 No. 1 (2013); 74-76
Brazilian Journal of Veterinary Research and Animal Science; v. 50 n. 1 (2013); 74-76
Brazilian Journal of Veterinary Research and Animal Science; V. 50 N. 1 (2013); 74-76
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
_version_ 1797051564411584512

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