Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses

Detalhes bibliográficos
Autor(a) principal: Garcia, José Fernando
Data de Publicação: 1996
Outros Autores: Gurgel, André Sanches do Amaral, Visintin, José Antonio, Lunge, Vagner Ricardo, Duarte, Marcelo Barbante, Bertolli, Jarbas Leonel
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/50181
Resumo: Twelve Quarter horses, descendants from Impressive line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Taq I restrictionenzyme. Accordingly the Hyperkalemic Periodic Paralysis (HYPP) genomic diagnosis was established. The tests revealed that 9 animals were carrier heterozygotes (N/H) and 3 normal homozygotes (N/N). Since the development of PCR methodology, it has been possible to diagnose the problem and plan ways to control the spreading of this defective gene in the population, through detection of carrier and normal animals.
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spelling Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horsesUtilização de marcadores de DNA para o diagnóstico genômico de animais domésticos: 2. Detecção da mutação pontual causadora da Paralisia Hipercalêmica Periódica (HYPP) em eqüinos da raça Quarto de MilhaParalysisHyperkalemiaHorsesEquidaeDNA.CavalosEquidaeDNAParalisiaHipercalemia.Twelve Quarter horses, descendants from Impressive line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Taq I restrictionenzyme. Accordingly the Hyperkalemic Periodic Paralysis (HYPP) genomic diagnosis was established. The tests revealed that 9 animals were carrier heterozygotes (N/H) and 3 normal homozygotes (N/N). Since the development of PCR methodology, it has been possible to diagnose the problem and plan ways to control the spreading of this defective gene in the population, through detection of carrier and normal animals.Analisaram-se 12 eqüinos da raça Quarto de Milha, descendentes da linhagem Impressive. Submeteram-se os DNAs, purificados a partir de leucócitos destes animais, à técnica de Polymerase Chain Reaction (PCR) e posterior digestão com a enzima de restrição Taq I. Estabeleceu-se, dessa maneira, o diagnóstico genômico da Paralisia Hipercalêmica Periódica (HYPP). Os exames revelaram que 9 animais eram portadores heterozigotos (N/H) e 3, normais homozigotos (N/N). A partir do desenvolvimento da metodologia de PCR tornou-se possível diagnosticar o problema e propor maneiras de controle do alastramento desse gene defectivo na população por meio da detecção de animais portadores e normais.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1996-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5018110.11606/issn.2318-3659.v33i3p136-139Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 Núm. 3 (1996); 136-139Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 No. 3 (1996); 136-139Brazilian Journal of Veterinary Research and Animal Science; v. 33 n. 3 (1996); 136-139Brazilian Journal of Veterinary Research and Animal Science; V. 33 N. 3 (1996); 136-1391678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/50181/54296Garcia, José FernandoGurgel, André Sanches do AmaralVisintin, José AntonioLunge, Vagner RicardoDuarte, Marcelo BarbanteBertolli, Jarbas Leonelinfo:eu-repo/semantics/openAccess2020-06-23T04:49:53Zoai:revistas.usp.br:article/50181Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:14.918660Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses
Utilização de marcadores de DNA para o diagnóstico genômico de animais domésticos: 2. Detecção da mutação pontual causadora da Paralisia Hipercalêmica Periódica (HYPP) em eqüinos da raça Quarto de Milha
title Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses
spellingShingle Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses
Garcia, José Fernando
Paralysis
Hyperkalemia
Horses
Equidae
DNA.
Cavalos
Equidae
DNA
Paralisia
Hipercalemia.
title_short Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses
title_full Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses
title_fullStr Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses
title_full_unstemmed Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses
title_sort Use of DNA markers for genomic diagnosis of domestic animals: 2. Detection of point mutation causing Hyperkalemic Periodic Paralysis (HYPP) in Quarter horses
author Garcia, José Fernando
author_facet Garcia, José Fernando
Gurgel, André Sanches do Amaral
Visintin, José Antonio
Lunge, Vagner Ricardo
Duarte, Marcelo Barbante
Bertolli, Jarbas Leonel
author_role author
author2 Gurgel, André Sanches do Amaral
Visintin, José Antonio
Lunge, Vagner Ricardo
Duarte, Marcelo Barbante
Bertolli, Jarbas Leonel
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Garcia, José Fernando
Gurgel, André Sanches do Amaral
Visintin, José Antonio
Lunge, Vagner Ricardo
Duarte, Marcelo Barbante
Bertolli, Jarbas Leonel
dc.subject.por.fl_str_mv Paralysis
Hyperkalemia
Horses
Equidae
DNA.
Cavalos
Equidae
DNA
Paralisia
Hipercalemia.
topic Paralysis
Hyperkalemia
Horses
Equidae
DNA.
Cavalos
Equidae
DNA
Paralisia
Hipercalemia.
description Twelve Quarter horses, descendants from Impressive line, have been analyzed. DNAs, purified from leukocytes of those animals, were submitted to Polymerase Chain Reaction technique (PCR) and digested by Taq I restrictionenzyme. Accordingly the Hyperkalemic Periodic Paralysis (HYPP) genomic diagnosis was established. The tests revealed that 9 animals were carrier heterozygotes (N/H) and 3 normal homozygotes (N/N). Since the development of PCR methodology, it has been possible to diagnose the problem and plan ways to control the spreading of this defective gene in the population, through detection of carrier and normal animals.
publishDate 1996
dc.date.none.fl_str_mv 1996-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/50181
10.11606/issn.2318-3659.v33i3p136-139
url https://www.revistas.usp.br/bjvras/article/view/50181
identifier_str_mv 10.11606/issn.2318-3659.v33i3p136-139
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/50181/54296
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 Núm. 3 (1996); 136-139
Brazilian Journal of Veterinary Research and Animal Science; Vol. 33 No. 3 (1996); 136-139
Brazilian Journal of Veterinary Research and Animal Science; v. 33 n. 3 (1996); 136-139
Brazilian Journal of Veterinary Research and Animal Science; V. 33 N. 3 (1996); 136-139
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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