Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs
Autor(a) principal: | |
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Data de Publicação: | 1998 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | por |
Título da fonte: | Brazilian Journal of Veterinary Research and Animal Science |
Texto Completo: | https://www.revistas.usp.br/bjvras/article/view/5700 |
Resumo: | An experimental inactivated, oil assisted Aujeszky's disease virus vaccine was tested for its ability to induce immunity, clinical protection level and reduction of viral infection after challenge by the nasal route. Groups of 6 pigs, 45 days-old, were vaccinated once or twice, by either intramuscular or subcutaneous routes. A group of unvaccinated animals was used as control. All the vaccinated pigs developed neutralizing antibodies, detected by serum-neutralization test. In pigs vaccinated twice the levels of neutralizing antibodies were higher than in pigs vaccinated once, and this difference was statistically significant. In vaccinated animals, the severity of clinical signs decreased with increasing antibodies titres. Aujeszky's disease virus was detected in tonsils of vaccinated and unvaccinated pigs by immunofluorescence method. However, the rate of positive tonsils in pigs vaccinated twice was reduced when compared with unvaccinated pigs on the 2nd and 7th days post-infection. The level of infection was statistically reduced in twice vaccinated pigs by subcutaneous route as compared with vaccinated once and unvaccinated animals. |
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Brazilian Journal of Veterinary Research and Animal Science |
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Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigsEficácia de vacina inativada contra a doença de Aujeszky: infecção experimental de suínosDoença de AujeszkyVacinas inativadasTonsilasAujeszky’s diseaseInactivated vaccinesTonsilsAn experimental inactivated, oil assisted Aujeszky's disease virus vaccine was tested for its ability to induce immunity, clinical protection level and reduction of viral infection after challenge by the nasal route. Groups of 6 pigs, 45 days-old, were vaccinated once or twice, by either intramuscular or subcutaneous routes. A group of unvaccinated animals was used as control. All the vaccinated pigs developed neutralizing antibodies, detected by serum-neutralization test. In pigs vaccinated twice the levels of neutralizing antibodies were higher than in pigs vaccinated once, and this difference was statistically significant. In vaccinated animals, the severity of clinical signs decreased with increasing antibodies titres. Aujeszky's disease virus was detected in tonsils of vaccinated and unvaccinated pigs by immunofluorescence method. However, the rate of positive tonsils in pigs vaccinated twice was reduced when compared with unvaccinated pigs on the 2nd and 7th days post-infection. The level of infection was statistically reduced in twice vaccinated pigs by subcutaneous route as compared with vaccinated once and unvaccinated animals.Uma vacina experimental contra a doença de Aujeszky (DA), inativada e adsorvida em adjuvante oleoso, foi testada em relação à indução de imunidade, grau de proteção clínica e capacidade de reduzir a infecção, após desafio pela via intranasal. Grupos de 6 suínos, com 45 dias de idade, foram vacinados com uma ou duas doses de vacina, pelas vias intramuscular ou subcutânea, sendo mantido um grupo-testemunha, sem vacinação. Todos os suínos vacinados apresentaram títulos de anticorpos neutralizantes, detectáveis através da prova de soroneutralização, sendo estatisticamente superiores nos animais que receberam duas doses de vacina. A vacinação preveniu as manifestações clínicas da doença, após desafio, principalmente nos animais que receberam duas doses de vacina quando comparado com os que receberam 1 dose. Em todos os animais, vacinados e não-vacinados, foi possível a detecção do vírus da doença de Aujeszky (VDA) nas amígdalas, através da técnica de imunofluorescência direta. A porcentagem de amígdalas positivas nos suínos vacinados com duas doses foi inferior quando comparada àquela de suínos não-vacinados, no 2º e 7º dias pós-infecção. Em suínos vacinados com duas doses de vacina, pela via subcutânea, a taxa de infecção foi estatisticamente menor que naqueles vacinados com uma dose, pelas vias intramuscular ou subcutânea, e não-vacinados.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/570010.1590/S1413-95961998000400009Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 Núm. 4 (1998); 188-194Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 No. 4 (1998); 188-194Brazilian Journal of Veterinary Research and Animal Science; v. 35 n. 4 (1998); 188-194Brazilian Journal of Veterinary Research and Animal Science; V. 35 N. 4 (1998); 188-1941678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/5700/7231Cunha, Elenice Maria SequetinPeixoto, Zélia Maria PinheiroKotait, IvaneteBersano, Josete GarciaIshizuka, Massaio MisunoBaccaro, Maria Reginainfo:eu-repo/semantics/openAccess2020-06-23T04:45:34Zoai:revistas.usp.br:article/5700Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:18.016835Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false |
dc.title.none.fl_str_mv |
Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs Eficácia de vacina inativada contra a doença de Aujeszky: infecção experimental de suínos |
title |
Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs |
spellingShingle |
Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs Cunha, Elenice Maria Sequetin Doença de Aujeszky Vacinas inativadas Tonsilas Aujeszky’s disease Inactivated vaccines Tonsils |
title_short |
Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs |
title_full |
Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs |
title_fullStr |
Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs |
title_full_unstemmed |
Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs |
title_sort |
Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs |
author |
Cunha, Elenice Maria Sequetin |
author_facet |
Cunha, Elenice Maria Sequetin Peixoto, Zélia Maria Pinheiro Kotait, Ivanete Bersano, Josete Garcia Ishizuka, Massaio Misuno Baccaro, Maria Regina |
author_role |
author |
author2 |
Peixoto, Zélia Maria Pinheiro Kotait, Ivanete Bersano, Josete Garcia Ishizuka, Massaio Misuno Baccaro, Maria Regina |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Cunha, Elenice Maria Sequetin Peixoto, Zélia Maria Pinheiro Kotait, Ivanete Bersano, Josete Garcia Ishizuka, Massaio Misuno Baccaro, Maria Regina |
dc.subject.por.fl_str_mv |
Doença de Aujeszky Vacinas inativadas Tonsilas Aujeszky’s disease Inactivated vaccines Tonsils |
topic |
Doença de Aujeszky Vacinas inativadas Tonsilas Aujeszky’s disease Inactivated vaccines Tonsils |
description |
An experimental inactivated, oil assisted Aujeszky's disease virus vaccine was tested for its ability to induce immunity, clinical protection level and reduction of viral infection after challenge by the nasal route. Groups of 6 pigs, 45 days-old, were vaccinated once or twice, by either intramuscular or subcutaneous routes. A group of unvaccinated animals was used as control. All the vaccinated pigs developed neutralizing antibodies, detected by serum-neutralization test. In pigs vaccinated twice the levels of neutralizing antibodies were higher than in pigs vaccinated once, and this difference was statistically significant. In vaccinated animals, the severity of clinical signs decreased with increasing antibodies titres. Aujeszky's disease virus was detected in tonsils of vaccinated and unvaccinated pigs by immunofluorescence method. However, the rate of positive tonsils in pigs vaccinated twice was reduced when compared with unvaccinated pigs on the 2nd and 7th days post-infection. The level of infection was statistically reduced in twice vaccinated pigs by subcutaneous route as compared with vaccinated once and unvaccinated animals. |
publishDate |
1998 |
dc.date.none.fl_str_mv |
1998-01-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5700 10.1590/S1413-95961998000400009 |
url |
https://www.revistas.usp.br/bjvras/article/view/5700 |
identifier_str_mv |
10.1590/S1413-95961998000400009 |
dc.language.iso.fl_str_mv |
por |
language |
por |
dc.relation.none.fl_str_mv |
https://www.revistas.usp.br/bjvras/article/view/5700/7231 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
application/pdf |
dc.publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
publisher.none.fl_str_mv |
Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia |
dc.source.none.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 Núm. 4 (1998); 188-194 Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 No. 4 (1998); 188-194 Brazilian Journal of Veterinary Research and Animal Science; v. 35 n. 4 (1998); 188-194 Brazilian Journal of Veterinary Research and Animal Science; V. 35 N. 4 (1998); 188-194 1678-4456 1413-9596 reponame:Brazilian Journal of Veterinary Research and Animal Science instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) instacron:USP |
instname_str |
Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
instacron_str |
USP |
institution |
USP |
reponame_str |
Brazilian Journal of Veterinary Research and Animal Science |
collection |
Brazilian Journal of Veterinary Research and Animal Science |
repository.name.fl_str_mv |
Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP) |
repository.mail.fl_str_mv |
bjvras@usp.br |
_version_ |
1797051554563358720 |