Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs

Detalhes bibliográficos
Autor(a) principal: Cunha, Elenice Maria Sequetin
Data de Publicação: 1998
Outros Autores: Peixoto, Zélia Maria Pinheiro, Kotait, Ivanete, Bersano, Josete Garcia, Ishizuka, Massaio Misuno, Baccaro, Maria Regina
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/5700
Resumo: An experimental inactivated, oil assisted Aujeszky's disease virus vaccine was tested for its ability to induce immunity, clinical protection level and reduction of viral infection after challenge by the nasal route. Groups of 6 pigs, 45 days-old, were vaccinated once or twice, by either intramuscular or subcutaneous routes. A group of unvaccinated animals was used as control. All the vaccinated pigs developed neutralizing antibodies, detected by serum-neutralization test. In pigs vaccinated twice the levels of neutralizing antibodies were higher than in pigs vaccinated once, and this difference was statistically significant. In vaccinated animals, the severity of clinical signs decreased with increasing antibodies titres. Aujeszky's disease virus was detected in tonsils of vaccinated and unvaccinated pigs by immunofluorescence method. However, the rate of positive tonsils in pigs vaccinated twice was reduced when compared with unvaccinated pigs on the 2nd and 7th days post-infection. The level of infection was statistically reduced in twice vaccinated pigs by subcutaneous route as compared with vaccinated once and unvaccinated animals.
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spelling Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigsEficácia de vacina inativada contra a doença de Aujeszky: infecção experimental de suínosDoença de AujeszkyVacinas inativadasTonsilasAujeszky’s diseaseInactivated vaccinesTonsilsAn experimental inactivated, oil assisted Aujeszky's disease virus vaccine was tested for its ability to induce immunity, clinical protection level and reduction of viral infection after challenge by the nasal route. Groups of 6 pigs, 45 days-old, were vaccinated once or twice, by either intramuscular or subcutaneous routes. A group of unvaccinated animals was used as control. All the vaccinated pigs developed neutralizing antibodies, detected by serum-neutralization test. In pigs vaccinated twice the levels of neutralizing antibodies were higher than in pigs vaccinated once, and this difference was statistically significant. In vaccinated animals, the severity of clinical signs decreased with increasing antibodies titres. Aujeszky's disease virus was detected in tonsils of vaccinated and unvaccinated pigs by immunofluorescence method. However, the rate of positive tonsils in pigs vaccinated twice was reduced when compared with unvaccinated pigs on the 2nd and 7th days post-infection. The level of infection was statistically reduced in twice vaccinated pigs by subcutaneous route as compared with vaccinated once and unvaccinated animals.Uma vacina experimental contra a doença de Aujeszky (DA), inativada e adsorvida em adjuvante oleoso, foi testada em relação à indução de imunidade, grau de proteção clínica e capacidade de reduzir a infecção, após desafio pela via intranasal. Grupos de 6 suínos, com 45 dias de idade, foram vacinados com uma ou duas doses de vacina, pelas vias intramuscular ou subcutânea, sendo mantido um grupo-testemunha, sem vacinação. Todos os suínos vacinados apresentaram títulos de anticorpos neutralizantes, detectáveis através da prova de soroneutralização, sendo estatisticamente superiores nos animais que receberam duas doses de vacina. A vacinação preveniu as manifestações clínicas da doença, após desafio, principalmente nos animais que receberam duas doses de vacina quando comparado com os que receberam 1 dose. Em todos os animais, vacinados e não-vacinados, foi possível a detecção do vírus da doença de Aujeszky (VDA) nas amígdalas, através da técnica de imunofluorescência direta. A porcentagem de amígdalas positivas nos suínos vacinados com duas doses foi inferior quando comparada àquela de suínos não-vacinados, no 2º e 7º dias pós-infecção. Em suínos vacinados com duas doses de vacina, pela via subcutânea, a taxa de infecção foi estatisticamente menor que naqueles vacinados com uma dose, pelas vias intramuscular ou subcutânea, e não-vacinados.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1998-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/570010.1590/S1413-95961998000400009Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 Núm. 4 (1998); 188-194Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 No. 4 (1998); 188-194Brazilian Journal of Veterinary Research and Animal Science; v. 35 n. 4 (1998); 188-194Brazilian Journal of Veterinary Research and Animal Science; V. 35 N. 4 (1998); 188-1941678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/5700/7231Cunha, Elenice Maria SequetinPeixoto, Zélia Maria PinheiroKotait, IvaneteBersano, Josete GarciaIshizuka, Massaio MisunoBaccaro, Maria Reginainfo:eu-repo/semantics/openAccess2020-06-23T04:45:34Zoai:revistas.usp.br:article/5700Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:42:18.016835Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs
Eficácia de vacina inativada contra a doença de Aujeszky: infecção experimental de suínos
title Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs
spellingShingle Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs
Cunha, Elenice Maria Sequetin
Doença de Aujeszky
Vacinas inativadas
Tonsilas
Aujeszky’s disease
Inactivated vaccines
Tonsils
title_short Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs
title_full Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs
title_fullStr Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs
title_full_unstemmed Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs
title_sort Efficacy of an inactivated Aujeszky's disease virus vaccine: experimental infection of pigs
author Cunha, Elenice Maria Sequetin
author_facet Cunha, Elenice Maria Sequetin
Peixoto, Zélia Maria Pinheiro
Kotait, Ivanete
Bersano, Josete Garcia
Ishizuka, Massaio Misuno
Baccaro, Maria Regina
author_role author
author2 Peixoto, Zélia Maria Pinheiro
Kotait, Ivanete
Bersano, Josete Garcia
Ishizuka, Massaio Misuno
Baccaro, Maria Regina
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Cunha, Elenice Maria Sequetin
Peixoto, Zélia Maria Pinheiro
Kotait, Ivanete
Bersano, Josete Garcia
Ishizuka, Massaio Misuno
Baccaro, Maria Regina
dc.subject.por.fl_str_mv Doença de Aujeszky
Vacinas inativadas
Tonsilas
Aujeszky’s disease
Inactivated vaccines
Tonsils
topic Doença de Aujeszky
Vacinas inativadas
Tonsilas
Aujeszky’s disease
Inactivated vaccines
Tonsils
description An experimental inactivated, oil assisted Aujeszky's disease virus vaccine was tested for its ability to induce immunity, clinical protection level and reduction of viral infection after challenge by the nasal route. Groups of 6 pigs, 45 days-old, were vaccinated once or twice, by either intramuscular or subcutaneous routes. A group of unvaccinated animals was used as control. All the vaccinated pigs developed neutralizing antibodies, detected by serum-neutralization test. In pigs vaccinated twice the levels of neutralizing antibodies were higher than in pigs vaccinated once, and this difference was statistically significant. In vaccinated animals, the severity of clinical signs decreased with increasing antibodies titres. Aujeszky's disease virus was detected in tonsils of vaccinated and unvaccinated pigs by immunofluorescence method. However, the rate of positive tonsils in pigs vaccinated twice was reduced when compared with unvaccinated pigs on the 2nd and 7th days post-infection. The level of infection was statistically reduced in twice vaccinated pigs by subcutaneous route as compared with vaccinated once and unvaccinated animals.
publishDate 1998
dc.date.none.fl_str_mv 1998-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5700
10.1590/S1413-95961998000400009
url https://www.revistas.usp.br/bjvras/article/view/5700
identifier_str_mv 10.1590/S1413-95961998000400009
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/5700/7231
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 Núm. 4 (1998); 188-194
Brazilian Journal of Veterinary Research and Animal Science; Vol. 35 No. 4 (1998); 188-194
Brazilian Journal of Veterinary Research and Animal Science; v. 35 n. 4 (1998); 188-194
Brazilian Journal of Veterinary Research and Animal Science; V. 35 N. 4 (1998); 188-194
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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