Dermatofitoses e leveduroses de cães e gatos. Aspectos diagnósticos

Detalhes bibliográficos
Autor(a) principal: Dubugras, Maria Theresa Bonilha
Data de Publicação: 1992
Outros Autores: Larsson, Carlos Eduardo, Ledon, Ana Luiza Basso Penteado, Gambale, Walderez
Tipo de documento: Artigo
Idioma: por
Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
Texto Completo: https://www.revistas.usp.br/bjvras/article/view/51994
Resumo: For the diagnosis of superficial mycoses, the clinical aspects and cultivation of fungi for further identification are usually considered. Identification, however, is time consuming and at least 4 weeks are needed for a positive laboratory report. In Brazil, the use of Wood’s light is not a routine screen test, especially in Veterinary Medicine. Thus, the present paper has the aim to evaluate the availability, the sensitivity and the specificity of the Wood's light in detecting fungal diseases because not all dermatomycoses are readily detected by this method. The fluorescence results were compared to those of fungi cultures of hair specimens from animals suspected of dermatophytes or yeast infections. The culture media used were the Sabouraud and the Mycose I Agar. From February 1989 to April 1990 the Dermatology Service of the University Veterinary Teaching Hospital attended 282 animals presenting lesions very suggestive of fungal diseases. From these, 162 (57.4%) were dogs and the remaining 120 (42.5%) were cats, of both sexes and of various ages and breeds. After performing dermatologie examination, the lesions were submitted to the Wood's light (250 nm) and fluorescence was observed in 70 (24.5%) cases. After the inoculation and in vitro cultivation of clinical specimens from either fluorescent and non-fluorescent lesions, positive fungal growth was found in 109 cases, 103 of these identified as dermatophytes or pathogenic yeast. The clear fluorescence was found only in Microsporum canis infection. The results of fluorescence and fungal growth were in accordance in 64.5% of the cases. The efficiency of the Wood's light in dogs and cats, compared to the mycological examination was respectively: sensitivity - 39.1% and 73.8%; specificity - 89.2% and 80.7%; possibility of diagnosis - 82.0% and 78.0%; predictable value of positive test - 37.5% and 67.3%; and predictable value of negative test - 89.8% and 85.1%. Epidemiologic aspects of dermalophytoses and yeast infections and fungi species isolated in cultures are furthermore discussed.
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spelling Dermatofitoses e leveduroses de cães e gatos. Aspectos diagnósticosDiagnostic aspects of dermalophytoses and superficial mycoses of dogs and catsDermatomicose (cães)Dermatomicose (gatos)MicrosporumTricophytonDiagnósticoDermatomycoses of dogsDermatomycoses of catsMicrosporumTricophytonDiagnosisFor the diagnosis of superficial mycoses, the clinical aspects and cultivation of fungi for further identification are usually considered. Identification, however, is time consuming and at least 4 weeks are needed for a positive laboratory report. In Brazil, the use of Wood’s light is not a routine screen test, especially in Veterinary Medicine. Thus, the present paper has the aim to evaluate the availability, the sensitivity and the specificity of the Wood's light in detecting fungal diseases because not all dermatomycoses are readily detected by this method. The fluorescence results were compared to those of fungi cultures of hair specimens from animals suspected of dermatophytes or yeast infections. The culture media used were the Sabouraud and the Mycose I Agar. From February 1989 to April 1990 the Dermatology Service of the University Veterinary Teaching Hospital attended 282 animals presenting lesions very suggestive of fungal diseases. From these, 162 (57.4%) were dogs and the remaining 120 (42.5%) were cats, of both sexes and of various ages and breeds. After performing dermatologie examination, the lesions were submitted to the Wood's light (250 nm) and fluorescence was observed in 70 (24.5%) cases. After the inoculation and in vitro cultivation of clinical specimens from either fluorescent and non-fluorescent lesions, positive fungal growth was found in 109 cases, 103 of these identified as dermatophytes or pathogenic yeast. The clear fluorescence was found only in Microsporum canis infection. The results of fluorescence and fungal growth were in accordance in 64.5% of the cases. The efficiency of the Wood's light in dogs and cats, compared to the mycological examination was respectively: sensitivity - 39.1% and 73.8%; specificity - 89.2% and 80.7%; possibility of diagnosis - 82.0% and 78.0%; predictable value of positive test - 37.5% and 67.3%; and predictable value of negative test - 89.8% and 85.1%. Epidemiologic aspects of dermalophytoses and yeast infections and fungi species isolated in cultures are furthermore discussed.Para o diagnóstico das infecções fúngicas superficiais recorre-se aos aspectos clínicos, exame direto, e de cultivo micológico. Este último exige período mínimo de quatro semanas para a obtenção dos resultados. O exame com o uso de luz fluorescente (lâmpada de Wood) não é habitualmente utilizado como teste de triagem em medicina veterinária, no Brasil. Objetiva-se avaliar a praticabilidade, sensibilidade e especificidade do uso da luz de Wood, com fins diagnósticos, comparando-se os resultados obtidos, com cultivo micológico em ágares Sabouraud e Mycosel (DIFCO), de pelame colhido de animais suspeitos de estarem infectados por dermatófitos ou leveduras. No período de fevereiro de 1989 a abril de 1990 atenderam-se no Serviço de Dermatologia do VCM/HOVET, 282 animais (162 cães - 57,4% e 120 gatos - 42,6%), com e sem precisa definição racial, de ambos os sexos e de diferentes faixas etárias, que apresentavam lesões sugestivas de infecção fúngica. Após o exame dermatológico direto, procedeu-se a exposição das lesões à luz de Wood (250 nm), assinalando-se fluorescência em 70 (24,5%) casos. Após a semeadura e o cultivo do material colhido das lesões, quer das fluorescentes como daquelas que não fluoresceram, verificou-se crescimento em 109 casos, sendo que, em 103 houve crescimento de dermatófitos ou leveduras patogênicos. Somente nos casos de infecção pelo Microsporum canis houve evidente fluorescência. Houve coincidência entre os resultados obtidos pela inspeção indireta (luz de Wood) e aqueles obtidos no cultivo micológico em 64,5% dos casos. A eficiência relativa da luz de Wood comparada ao exame micológico foi, respectivamente, para cães e gatos: sensibilidade -39,1% e 73,8%; especificidade - 89,2% e 80,7%; concordância - 82% e 78%; valor preditivo positivo - 37,5% e 67,3% e valor preditivo negativo - 89,8% e 85,1%. Discutem-se, ainda, os aspectos epidemiológicos das dermatofitoses e leveduroses e as espécies fúngicas isoladas no material cultivado.Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia1992-12-02info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://www.revistas.usp.br/bjvras/article/view/5199410.11606/issn.1678-4456.bjvras.1992.51994Brazilian Journal of Veterinary Research and Animal Science; Vol. 29 Núm. 2 (1992); 273-287Brazilian Journal of Veterinary Research and Animal Science; Vol. 29 No. 2 (1992); 273-287Brazilian Journal of Veterinary Research and Animal Science; v. 29 n. 2 (1992); 273-287Brazilian Journal of Veterinary Research and Animal Science; V. 29 N. 2 (1992); 273-2871678-44561413-9596reponame:Brazilian Journal of Veterinary Research and Animal Scienceinstname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)instacron:USPporhttps://www.revistas.usp.br/bjvras/article/view/51994/56044Dubugras, Maria Theresa BonilhaLarsson, Carlos EduardoLedon, Ana Luiza Basso PenteadoGambale, Walderezinfo:eu-repo/semantics/openAccess2020-06-23T04:55:05Zoai:revistas.usp.br:article/51994Revistahttps://www.revistas.usp.br/bjvrasPUBhttps://www.revistas.usp.br/bjvras/oaibjvras@usp.br1413-95961413-9596opendoar:https://www.revistas.usp.br/bjvras/index2023-01-12T16:43:25.377559Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)false
dc.title.none.fl_str_mv Dermatofitoses e leveduroses de cães e gatos. Aspectos diagnósticos
Diagnostic aspects of dermalophytoses and superficial mycoses of dogs and cats
title Dermatofitoses e leveduroses de cães e gatos. Aspectos diagnósticos
spellingShingle Dermatofitoses e leveduroses de cães e gatos. Aspectos diagnósticos
Dubugras, Maria Theresa Bonilha
Dermatomicose (cães)
Dermatomicose (gatos)
Microsporum
Tricophyton
Diagnóstico
Dermatomycoses of dogs
Dermatomycoses of cats
Microsporum
Tricophyton
Diagnosis
title_short Dermatofitoses e leveduroses de cães e gatos. Aspectos diagnósticos
title_full Dermatofitoses e leveduroses de cães e gatos. Aspectos diagnósticos
title_fullStr Dermatofitoses e leveduroses de cães e gatos. Aspectos diagnósticos
title_full_unstemmed Dermatofitoses e leveduroses de cães e gatos. Aspectos diagnósticos
title_sort Dermatofitoses e leveduroses de cães e gatos. Aspectos diagnósticos
author Dubugras, Maria Theresa Bonilha
author_facet Dubugras, Maria Theresa Bonilha
Larsson, Carlos Eduardo
Ledon, Ana Luiza Basso Penteado
Gambale, Walderez
author_role author
author2 Larsson, Carlos Eduardo
Ledon, Ana Luiza Basso Penteado
Gambale, Walderez
author2_role author
author
author
dc.contributor.author.fl_str_mv Dubugras, Maria Theresa Bonilha
Larsson, Carlos Eduardo
Ledon, Ana Luiza Basso Penteado
Gambale, Walderez
dc.subject.por.fl_str_mv Dermatomicose (cães)
Dermatomicose (gatos)
Microsporum
Tricophyton
Diagnóstico
Dermatomycoses of dogs
Dermatomycoses of cats
Microsporum
Tricophyton
Diagnosis
topic Dermatomicose (cães)
Dermatomicose (gatos)
Microsporum
Tricophyton
Diagnóstico
Dermatomycoses of dogs
Dermatomycoses of cats
Microsporum
Tricophyton
Diagnosis
description For the diagnosis of superficial mycoses, the clinical aspects and cultivation of fungi for further identification are usually considered. Identification, however, is time consuming and at least 4 weeks are needed for a positive laboratory report. In Brazil, the use of Wood’s light is not a routine screen test, especially in Veterinary Medicine. Thus, the present paper has the aim to evaluate the availability, the sensitivity and the specificity of the Wood's light in detecting fungal diseases because not all dermatomycoses are readily detected by this method. The fluorescence results were compared to those of fungi cultures of hair specimens from animals suspected of dermatophytes or yeast infections. The culture media used were the Sabouraud and the Mycose I Agar. From February 1989 to April 1990 the Dermatology Service of the University Veterinary Teaching Hospital attended 282 animals presenting lesions very suggestive of fungal diseases. From these, 162 (57.4%) were dogs and the remaining 120 (42.5%) were cats, of both sexes and of various ages and breeds. After performing dermatologie examination, the lesions were submitted to the Wood's light (250 nm) and fluorescence was observed in 70 (24.5%) cases. After the inoculation and in vitro cultivation of clinical specimens from either fluorescent and non-fluorescent lesions, positive fungal growth was found in 109 cases, 103 of these identified as dermatophytes or pathogenic yeast. The clear fluorescence was found only in Microsporum canis infection. The results of fluorescence and fungal growth were in accordance in 64.5% of the cases. The efficiency of the Wood's light in dogs and cats, compared to the mycological examination was respectively: sensitivity - 39.1% and 73.8%; specificity - 89.2% and 80.7%; possibility of diagnosis - 82.0% and 78.0%; predictable value of positive test - 37.5% and 67.3%; and predictable value of negative test - 89.8% and 85.1%. Epidemiologic aspects of dermalophytoses and yeast infections and fungi species isolated in cultures are furthermore discussed.
publishDate 1992
dc.date.none.fl_str_mv 1992-12-02
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/51994
10.11606/issn.1678-4456.bjvras.1992.51994
url https://www.revistas.usp.br/bjvras/article/view/51994
identifier_str_mv 10.11606/issn.1678-4456.bjvras.1992.51994
dc.language.iso.fl_str_mv por
language por
dc.relation.none.fl_str_mv https://www.revistas.usp.br/bjvras/article/view/51994/56044
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
publisher.none.fl_str_mv Universidade de São Paulo. Faculdade de Medicina Veterinária e Zootecnia
dc.source.none.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science; Vol. 29 Núm. 2 (1992); 273-287
Brazilian Journal of Veterinary Research and Animal Science; Vol. 29 No. 2 (1992); 273-287
Brazilian Journal of Veterinary Research and Animal Science; v. 29 n. 2 (1992); 273-287
Brazilian Journal of Veterinary Research and Animal Science; V. 29 N. 2 (1992); 273-287
1678-4456
1413-9596
reponame:Brazilian Journal of Veterinary Research and Animal Science
instname:Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron:USP
instname_str Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
instacron_str USP
institution USP
reponame_str Brazilian Journal of Veterinary Research and Animal Science
collection Brazilian Journal of Veterinary Research and Animal Science
repository.name.fl_str_mv Brazilian Journal of Veterinary Research and Animal Science - Faculdade de Medicina Veterinária e Zootecnia da Universidade de São Paulo (FMVZ-USP)
repository.mail.fl_str_mv bjvras@usp.br
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