Study of the immunopathogenesis of central nervous system lesions in dogs naturally affected by visceral leishmaniasis

• Autor(a) principal: Schwardt, Tatianna Frate
• Data de Publicação: 2012
• Outros Autores: Vides, Juliana Peloi, Braga, Eveline Tozzi, Pacheco, Acácio Duarte, Langoni, Hélio, Generoso, Diego, Machado, Gisele Fabrino, Laurenti, Márcia Dalastra, Marcondes, Mary
• Tipo de documento: Artigo
• Idioma: por
• Título da fonte: Brazilian Journal of Veterinary Research and Animal Science
• Texto Completo: https://www.revistas.usp.br/bjvras/article/view/54037
• topic: TCD3; TCD4; TCD8; Macrófagos; Leishmania; TCD3; TCD4; TCD8; Macrophages; Leishmania (Leishmania) infantum chagasi

Visceral leishmaniasis in dogs is described as a chronic disease whose main symptoms are progressive weigth loss, cachexy and dermatologic lesions. Recently, the disease has been associated to neurologic disorders. A total of 40 dogs with visceral leishmaniasis were divided into two groups. The first composed of dogs without neurological signs (n=30) and the second by dogs with neurological disorders (n=10). Brain samples were collected, stored in 10% buffered formalin and subjected to immunohistochemical examination for amastigotes forms of Leishmania (Leishmania) infantum chagasi, CD3+, CD4+ and CD8+ T lymphocytes and macrophages. Imunnohistochemistry evaluation revealed no amastigote forms of the parasite. CD3+ T lymphocytes were present in 24/30 (80%) dogs without neurological signs and in all dogs from the second group (p=0.0011). CD4+ and CD8+ were rarely observed, with CD4+ immunostaining in 10/40 (25%) dogs, from which half of them had neurological disease (p=0.0090). The presence of CD8+ was detected only in 4/10 (40%) dogs from neurological group (p=0.0021). Macrophages were detected in 38/40 (95%) dogs, without significant differences between groups (p=0.7664).